Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni
Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, two of the most common gastric Cryptosporidium spp. infecting mammals. In this study, we screened the C. muris whole-genome sequenci...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2011-01, Vol.49 (1), p.34-41 |
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| creator | Feng, Yaoyu Yang, Wenli Ryan, Una Zhang, Longxian Kváč, Martin Koudela, Břetislav Modrý, David Li, Na Fayer, Ronald Xiao, Lihua |
| description | Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, two of the most common gastric Cryptosporidium spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data for microsatellite and minisatellite sequences. Among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were eventually chosen. DNA sequence analyses of 27 C. muris and 17 C. andersoni DNA preparations showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all four loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Thus, an MLST technique was developed for the high-resolution typing of C. muris and C. andersoni. It should be useful for the characterization of the population genetics and transmission of gastric Cryptosporidium spp. |
| doi_str_mv | 10.1128/JCM.01329-10 |
| format | Article |
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In this study, we screened the C. muris whole-genome sequencing data for microsatellite and minisatellite sequences. Among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were eventually chosen. DNA sequence analyses of 27 C. muris and 17 C. andersoni DNA preparations showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all four loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Thus, an MLST technique was developed for the high-resolution typing of C. muris and C. andersoni. It should be useful for the characterization of the population genetics and transmission of gastric Cryptosporidium spp.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.01329-10</identifier><identifier>PMID: 20980577</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Biological and medical sciences ; cryptosporidiosis ; Cryptosporidiosis - diagnosis ; Cryptosporidiosis - veterinary ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium andersoni ; Cryptosporidium muris ; DNA ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; Fundamental and applied biological sciences. Psychology ; Humans ; loci ; Mammals ; Microbiology ; Microsatellite Repeats ; mole rats ; Molecular Sequence Data ; Multilocus Sequence Typing - methods ; Parasitology ; Parasitology - methods ; polymerase chain reaction ; population genetics ; sequence analysis ; Sequence Analysis, DNA</subject><ispartof>Journal of Clinical Microbiology, 2011-01, Vol.49 (1), p.34-41</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011, American Society for Microbiology 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-f87063d0305bfd66daf43ea849d95fd2d0360a99b3d07363a297c4bdf07499823</citedby><cites>FETCH-LOGICAL-c493t-f87063d0305bfd66daf43ea849d95fd2d0360a99b3d07363a297c4bdf07499823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020410/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020410/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23760626$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20980577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Yaoyu</creatorcontrib><creatorcontrib>Yang, Wenli</creatorcontrib><creatorcontrib>Ryan, Una</creatorcontrib><creatorcontrib>Zhang, Longxian</creatorcontrib><creatorcontrib>Kváč, Martin</creatorcontrib><creatorcontrib>Koudela, Břetislav</creatorcontrib><creatorcontrib>Modrý, David</creatorcontrib><creatorcontrib>Li, Na</creatorcontrib><creatorcontrib>Fayer, Ronald</creatorcontrib><creatorcontrib>Xiao, Lihua</creatorcontrib><title>Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, two of the most common gastric Cryptosporidium spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data for microsatellite and minisatellite sequences. Among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were eventually chosen. DNA sequence analyses of 27 C. muris and 17 C. andersoni DNA preparations showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all four loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Thus, an MLST technique was developed for the high-resolution typing of C. muris and C. andersoni. It should be useful for the characterization of the population genetics and transmission of gastric Cryptosporidium spp.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>cryptosporidiosis</subject><subject>Cryptosporidiosis - diagnosis</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium andersoni</subject><subject>Cryptosporidium muris</subject><subject>DNA</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>loci</subject><subject>Mammals</subject><subject>Microbiology</subject><subject>Microsatellite Repeats</subject><subject>mole rats</subject><subject>Molecular Sequence Data</subject><subject>Multilocus Sequence Typing - methods</subject><subject>Parasitology</subject><subject>Parasitology - methods</subject><subject>polymerase chain reaction</subject><subject>population genetics</subject><subject>sequence analysis</subject><subject>Sequence Analysis, DNA</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAYhC0Eokvhxhl8QVxIef0RO74goYXyoVYcupW4WV7H3nWVxMFOivbf43aXFsSFk6V3Ho1nNAg9J3BCCG3efl2enwBhVFUEHqAFAdVUQsD3h2gBoOqKECaP0JOcrwAI53X9GB3RAkEt5QJtP7hr18Wxd8OEo8cGn8_dFLpo54wv3I_ZDdbhVYwd9jHh1W4MwwYv026cYh5jCm2Ye9zPKWRshvYfpdxcynEIT9Ejb7rsnh3eY3R5-nG1_Fydffv0Zfn-rLJcsanyjQTBWmBQr30rRGs8Z840XLWq9i0tigCj1LowkglmqJKWr1sPkivVUHaM3u19x3ndu9aWXsl0ekyhN2mnown6b2UIW72J15oBBU6gGLw-GKRY6udJ9yFb13VmcHHOuhFc1Q2t_4OklNWN5LKQb_akTTHn5PxdHgL6ZkVdVtS3K-rbCC_-7HAH_56tAK8OgMnWdD6ZwYZ8zzEpQFBRuJd7bhs2258hOW1yr69sr3n5SDN-T3gTtdmUGfXlBS1JgCjGqOTsF_esut0</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Feng, Yaoyu</creator><creator>Yang, Wenli</creator><creator>Ryan, Una</creator><creator>Zhang, Longxian</creator><creator>Kváč, Martin</creator><creator>Koudela, Břetislav</creator><creator>Modrý, David</creator><creator>Li, Na</creator><creator>Fayer, Ronald</creator><creator>Xiao, Lihua</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni</title><author>Feng, Yaoyu ; Yang, Wenli ; Ryan, Una ; Zhang, Longxian ; Kváč, Martin ; Koudela, Břetislav ; Modrý, David ; Li, Na ; Fayer, Ronald ; Xiao, Lihua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-f87063d0305bfd66daf43ea849d95fd2d0360a99b3d07363a297c4bdf07499823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>cryptosporidiosis</topic><topic>Cryptosporidiosis - diagnosis</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium andersoni</topic><topic>Cryptosporidium muris</topic><topic>DNA</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>loci</topic><topic>Mammals</topic><topic>Microbiology</topic><topic>Microsatellite Repeats</topic><topic>mole rats</topic><topic>Molecular Sequence Data</topic><topic>Multilocus Sequence Typing - methods</topic><topic>Parasitology</topic><topic>Parasitology - methods</topic><topic>polymerase chain reaction</topic><topic>population genetics</topic><topic>sequence analysis</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Yaoyu</creatorcontrib><creatorcontrib>Yang, Wenli</creatorcontrib><creatorcontrib>Ryan, Una</creatorcontrib><creatorcontrib>Zhang, Longxian</creatorcontrib><creatorcontrib>Kváč, Martin</creatorcontrib><creatorcontrib>Koudela, Břetislav</creatorcontrib><creatorcontrib>Modrý, David</creatorcontrib><creatorcontrib>Li, Na</creatorcontrib><creatorcontrib>Fayer, Ronald</creatorcontrib><creatorcontrib>Xiao, Lihua</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Yaoyu</au><au>Yang, Wenli</au><au>Ryan, Una</au><au>Zhang, Longxian</au><au>Kváč, Martin</au><au>Koudela, Břetislav</au><au>Modrý, David</au><au>Li, Na</au><au>Fayer, Ronald</au><au>Xiao, Lihua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>49</volume><issue>1</issue><spage>34</spage><epage>41</epage><pages>34-41</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, two of the most common gastric Cryptosporidium spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data for microsatellite and minisatellite sequences. Among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were eventually chosen. DNA sequence analyses of 27 C. muris and 17 C. andersoni DNA preparations showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all four loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Thus, an MLST technique was developed for the high-resolution typing of C. muris and C. andersoni. It should be useful for the characterization of the population genetics and transmission of gastric Cryptosporidium spp.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20980577</pmid><doi>10.1128/JCM.01329-10</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences cryptosporidiosis Cryptosporidiosis - diagnosis Cryptosporidiosis - veterinary Cryptosporidium Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium andersoni Cryptosporidium muris DNA DNA, Protozoan - chemistry DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology Humans loci Mammals Microbiology Microsatellite Repeats mole rats Molecular Sequence Data Multilocus Sequence Typing - methods Parasitology Parasitology - methods polymerase chain reaction population genetics sequence analysis Sequence Analysis, DNA |
| title | Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni |
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