Creating Designed Zinc-Finger Nucleases with Minimal Cytotoxicity

Zinc-finger nucleases (ZFNs) have emerged as powerful tools for delivering a targeted genomic double-strand break (DSB) to either stimulate local homologous recombination with investigator-provided donor DNA or induce gene mutations at the site of cleavage in the absence of a donor by nonhomologous...

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Veröffentlicht in:	Journal of molecular biology 2011-01, Vol.405 (3), p.630-641
Hauptverfasser:	Ramalingam, Sivaprakash, Kandavelou, Karthikeyan, Rajenderan, Raja, Chandrasegaran, Srinivasan
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence cytotoxicity Deoxyribonucleases, Type II Site-Specific - chemistry Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - toxicity DNA DNA Breaks, Double-Stranded Gene Targeting genes Genetic Loci Genome engineering green fluorescent protein HEK293 Cells Homologous recombination Humans loci Molecular Sequence Data mutation Non-homologous end-joining Protein Engineering Protein Multimerization Receptors, CCR5 - genetics Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - toxicity restriction endonucleases Site-Specific modification Targeted cleavage Zinc Fingers
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