Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis
Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x=7; muskmelon (C. melo) and wild Cucumis species, x=12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygo...
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| creator | Skálová, Dagmar Ondřej, Vladan Doležalová, Ivana Navrátilová, Božena Lebeda, Aleš |
| description | Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x=7; muskmelon (C. melo) and wild Cucumis species, x=12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed. |
| doi_str_mv | 10.1155/2010/475432 |
| format | Article |
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Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2010/475432</identifier><identifier>PMID: 21234406</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Biological and medical sciences ; Biotechnology - methods ; Cell division ; Chromosome Duplication ; Colleges & universities ; Cucumis - genetics ; Cucumis - physiology ; DNA Shuffling - methods ; Efficiency ; Embryos ; Experiments ; Flow cytometry ; Flowers & plants ; Gene banks ; Genotype & phenotype ; Hybridization ; Hybridization, Genetic ; Medical sciences ; Methods ; Pharmacology. Drug treatments ; Plant introductions ; Plant sciences ; Ploidies ; Pollination - physiology ; Polyploidy ; Species Specificity</subject><ispartof>BioMed research international, 2010-01, Vol.2010 (2010), p.1-8</ispartof><rights>Copyright © 2010 Dagmar Skálová et al.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Dagmar Skálová et al. Dagmar Skálová et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2010 Dagmar Skálová et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-bcfbd93ac256ac3245359abfeb2c73c9b9bcfe27cbd2684ec82f6a78d6b1c4863</citedby><cites>FETCH-LOGICAL-c457t-bcfbd93ac256ac3245359abfeb2c73c9b9bcfe27cbd2684ec82f6a78d6b1c4863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014699/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014699/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25883536$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21234406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Stewart, Neal</contributor><creatorcontrib>Skálová, Dagmar</creatorcontrib><creatorcontrib>Ondřej, Vladan</creatorcontrib><creatorcontrib>Doležalová, Ivana</creatorcontrib><creatorcontrib>Navrátilová, Božena</creatorcontrib><creatorcontrib>Lebeda, Aleš</creatorcontrib><title>Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x=7; muskmelon (C. melo) and wild Cucumis species, x=12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed.</description><subject>Biological and medical sciences</subject><subject>Biotechnology - methods</subject><subject>Cell division</subject><subject>Chromosome Duplication</subject><subject>Colleges & universities</subject><subject>Cucumis - genetics</subject><subject>Cucumis - physiology</subject><subject>DNA Shuffling - methods</subject><subject>Efficiency</subject><subject>Embryos</subject><subject>Experiments</subject><subject>Flow cytometry</subject><subject>Flowers & plants</subject><subject>Gene banks</subject><subject>Genotype & phenotype</subject><subject>Hybridization</subject><subject>Hybridization, Genetic</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Pharmacology. 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Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>21234406</pmid><doi>10.1155/2010/475432</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Biotechnology - methods Cell division Chromosome Duplication Colleges & universities Cucumis - genetics Cucumis - physiology DNA Shuffling - methods Efficiency Embryos Experiments Flow cytometry Flowers & plants Gene banks Genotype & phenotype Hybridization Hybridization, Genetic Medical sciences Methods Pharmacology. Drug treatments Plant introductions Plant sciences Ploidies Pollination - physiology Polyploidy Species Specificity |
| title | Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis |
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