Involvement of host calpain in the invasion of Cryptosporidium parvum
Cryptosporidium parvum induces the formation of an actin-dense plaque which is essential for the successful invasion of epithelial cells. Host molecules that are involved in the regulation of this cytoskeleton reorganization are unknown. Here we identified that calcium-dependent thiol protease calpa...
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| Veröffentlicht in: | Microbes and infection 2011-01, Vol.13 (1), p.103-107 |
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| creator | Perez-Cordon, Gregorio Nie, Weijia Schmidt, Diane Tzipori, Saul Feng, Hanping |
| description | Cryptosporidium parvum induces the formation of an actin-dense plaque which is essential for the successful invasion of epithelial cells. Host molecules that are involved in the regulation of this cytoskeleton reorganization are unknown. Here we identified that calcium-dependent thiol protease calpain is critical for regulating parasite-induced actin polymerization.
C. parvum invasion induced activation of calpain. Inhibition of calpain activity by overexpression of the endogenous inhibitor calpastatin diminished the formation of the actin-dense plaque and decreased the initial invasion of parasites. Our data indicates a key role of calpain activity of host cell in
C. parvum infection via regulating cytoskeleton reorganization. |
| doi_str_mv | 10.1016/j.micinf.2010.10.007 |
| format | Article |
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C. parvum invasion induced activation of calpain. Inhibition of calpain activity by overexpression of the endogenous inhibitor calpastatin diminished the formation of the actin-dense plaque and decreased the initial invasion of parasites. Our data indicates a key role of calpain activity of host cell in
C. parvum infection via regulating cytoskeleton reorganization.</description><identifier>ISSN: 1286-4579</identifier><identifier>EISSN: 1769-714X</identifier><identifier>DOI: 10.1016/j.micinf.2010.10.007</identifier><identifier>PMID: 21087681</identifier><language>eng</language><publisher>Kidlington: Elsevier SAS</publisher><subject>Biological and medical sciences ; Calpain ; Calpain - antagonists & inhibitors ; Calpain - metabolism ; Cell Line, Tumor ; Cryptosporidiosis - immunology ; Cryptosporidiosis - parasitology ; Cryptosporidium parvum ; Cryptosporidium parvum - physiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Invasion ; Microbiology</subject><ispartof>Microbes and infection, 2011-01, Vol.13 (1), p.103-107</ispartof><rights>2010 Institut Pasteur</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Institut Pasteur. Published by Elsevier SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-329d854824ed9823b16d480b21dea418f73f3a80245ca198665d1cb58baa0c713</citedby><cites>FETCH-LOGICAL-c524t-329d854824ed9823b16d480b21dea418f73f3a80245ca198665d1cb58baa0c713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1286457910002662$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23726315$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21087681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perez-Cordon, Gregorio</creatorcontrib><creatorcontrib>Nie, Weijia</creatorcontrib><creatorcontrib>Schmidt, Diane</creatorcontrib><creatorcontrib>Tzipori, Saul</creatorcontrib><creatorcontrib>Feng, Hanping</creatorcontrib><title>Involvement of host calpain in the invasion of Cryptosporidium parvum</title><title>Microbes and infection</title><addtitle>Microbes Infect</addtitle><description>Cryptosporidium parvum induces the formation of an actin-dense plaque which is essential for the successful invasion of epithelial cells. Host molecules that are involved in the regulation of this cytoskeleton reorganization are unknown. Here we identified that calcium-dependent thiol protease calpain is critical for regulating parasite-induced actin polymerization.
C. parvum invasion induced activation of calpain. Inhibition of calpain activity by overexpression of the endogenous inhibitor calpastatin diminished the formation of the actin-dense plaque and decreased the initial invasion of parasites. Our data indicates a key role of calpain activity of host cell in
C. parvum infection via regulating cytoskeleton reorganization.</description><subject>Biological and medical sciences</subject><subject>Calpain</subject><subject>Calpain - antagonists & inhibitors</subject><subject>Calpain - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cryptosporidiosis - immunology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidium parvum</subject><subject>Cryptosporidium parvum - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Invasion</subject><subject>Microbiology</subject><issn>1286-4579</issn><issn>1769-714X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcGK1TAUDaI44-gfiHQjrvrMTdIk3QjyGHVgwI2Cu5AmqS-PNqlJW5i_N_U9Z3SjELjh3nMP556D0EvAO8DA3x53ozc-9DuCf7V2GItH6BIEb2sB7Nvj8ieS16wR7QV6lvMRY2gEZ0_RBQEsBZdwia5vwhqH1Y0uzFXsq0PMc2X0MGkfqvLmgytl1dnHsM336W6aY55i8tYvYzXptC7jc_Sk10N2L871Cn39cP1l_6m-_fzxZv_-tjYNYXNNSWtlwyRhzraS0A64ZRJ3BKzTDGQvaE-1xIQ1RkMrOW8smK6RndbYCKBX6N2Jd1q60VlTRCc9qCn5Uac7FbVXf0-CP6jvcVUUA2PAC8GbM0GKPxaXZzX6bNww6ODiklVxhVBoMPs_khDKAeQmip2QJsWck-vv9QBWW1TqqE5RqS2qrVuiKmuv_rzlful3NgXw-gzQuSTSJx2Mzw84KggvWh9MccX51buksvEuGGd9cmZWNvp_K_kJVxO0SQ</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Perez-Cordon, Gregorio</creator><creator>Nie, Weijia</creator><creator>Schmidt, Diane</creator><creator>Tzipori, Saul</creator><creator>Feng, Hanping</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Involvement of host calpain in the invasion of Cryptosporidium parvum</title><author>Perez-Cordon, Gregorio ; Nie, Weijia ; Schmidt, Diane ; Tzipori, Saul ; Feng, Hanping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-329d854824ed9823b16d480b21dea418f73f3a80245ca198665d1cb58baa0c713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>Calpain</topic><topic>Calpain - antagonists & inhibitors</topic><topic>Calpain - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cryptosporidiosis - immunology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidium parvum</topic><topic>Cryptosporidium parvum - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Invasion</topic><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perez-Cordon, Gregorio</creatorcontrib><creatorcontrib>Nie, Weijia</creatorcontrib><creatorcontrib>Schmidt, Diane</creatorcontrib><creatorcontrib>Tzipori, Saul</creatorcontrib><creatorcontrib>Feng, Hanping</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbes and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perez-Cordon, Gregorio</au><au>Nie, Weijia</au><au>Schmidt, Diane</au><au>Tzipori, Saul</au><au>Feng, Hanping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of host calpain in the invasion of Cryptosporidium parvum</atitle><jtitle>Microbes and infection</jtitle><addtitle>Microbes Infect</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>13</volume><issue>1</issue><spage>103</spage><epage>107</epage><pages>103-107</pages><issn>1286-4579</issn><eissn>1769-714X</eissn><abstract>Cryptosporidium parvum induces the formation of an actin-dense plaque which is essential for the successful invasion of epithelial cells. Host molecules that are involved in the regulation of this cytoskeleton reorganization are unknown. Here we identified that calcium-dependent thiol protease calpain is critical for regulating parasite-induced actin polymerization.
C. parvum invasion induced activation of calpain. Inhibition of calpain activity by overexpression of the endogenous inhibitor calpastatin diminished the formation of the actin-dense plaque and decreased the initial invasion of parasites. Our data indicates a key role of calpain activity of host cell in
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| ispartof | Microbes and infection, 2011-01, Vol.13 (1), p.103-107 |
| issn | 1286-4579 1769-714X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Calpain Calpain - antagonists & inhibitors Calpain - metabolism Cell Line, Tumor Cryptosporidiosis - immunology Cryptosporidiosis - parasitology Cryptosporidium parvum Cryptosporidium parvum - physiology Fundamental and applied biological sciences. Psychology Humans Invasion Microbiology |
| title | Involvement of host calpain in the invasion of Cryptosporidium parvum |
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