A Quantitative Study of the Effects of Chaotropic Agents, Surfactants, and Solvents on the Digestion Efficiency of Human Plasma Proteins by Trypsin

Plasma biomarkers studies are based on the differential expression of proteins between different treatment groups or between diseased and control populations. Most mass spectrometry-based methods of protein quantitation, however, are based on the detection and quantitation of peptides, not intact pr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of proteome research 2010-10, Vol.9 (10), p.5422-5437
Hauptverfasser:	Proc, Jennifer L, Kuzyk, Michael A, Hardie, Darryl B, Yang, Juncong, Smith, Derek S, Jackson, Angela M, Parker, Carol E, Borchers, Christoph H
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitriles - pharmacology Biocatalysis - drug effects Blood Proteins - metabolism Deoxycholic Acid - pharmacology Guanidine - pharmacology Humans Hydrolysis - drug effects Methanol - pharmacology Peptides - metabolism Proteomics - methods Reproducibility of Results Sodium Dodecyl Sulfate - pharmacology Tandem Mass Spectrometry - methods Trifluoroethanol - pharmacology Trypsin - metabolism Urea - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!