Effects of zinc ex vivo on taurine uptake in goldfish retinal cells
Taurine and zinc exert neurotrophic effects in the central nervous system. Current studies demonstrate that Na+/Cl- dependent neurotransmitter transporters, similar to that of taurine, are modulated by micromolar concentrations of zinc. This study examined the effect of zinc sulfate ex vivo on [3H]t...
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| Veröffentlicht in: | Journal of biomedical science 2010-08, Vol.17 Suppl 1 (Suppl 1), p.S13-S13 |
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| creator | Nusetti, Sonia Urbina, Mary Lima, Lucimey |
| description | Taurine and zinc exert neurotrophic effects in the central nervous system. Current studies demonstrate that Na+/Cl- dependent neurotransmitter transporters, similar to that of taurine, are modulated by micromolar concentrations of zinc. This study examined the effect of zinc sulfate ex vivo on [3H]taurine transport in goldfish retina.
Isolated cells were incubated in Ringer with zinc (0.1-100 microM). Taurine transport was done with 50 nM [3H]taurine or by isotopic dilution with taurine (0.001-1 mM) and 50 nM [3H]taurine.
Zinc reduced the capacity of taurine transport without changes in affinity, and caused a noncompetitive inhibition of high affinity taurine transport, with an EC50= 0.072 microM. The mechanism by which zinc affects taurine transport is unknown at the present.
There may be a binding site of zinc in the transporter that affects union or translocation of taurine, or possibly the formation of taurine-zinc complexes, rather than free zinc, could affect the operation of the transporter. |
| doi_str_mv | 10.1186/1423-0127-17-S1-S13 |
| format | Article |
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Isolated cells were incubated in Ringer with zinc (0.1-100 microM). Taurine transport was done with 50 nM [3H]taurine or by isotopic dilution with taurine (0.001-1 mM) and 50 nM [3H]taurine.
Zinc reduced the capacity of taurine transport without changes in affinity, and caused a noncompetitive inhibition of high affinity taurine transport, with an EC50= 0.072 microM. The mechanism by which zinc affects taurine transport is unknown at the present.
There may be a binding site of zinc in the transporter that affects union or translocation of taurine, or possibly the formation of taurine-zinc complexes, rather than free zinc, could affect the operation of the transporter.</description><identifier>ISSN: 1423-0127</identifier><identifier>ISSN: 1021-7770</identifier><identifier>EISSN: 1423-0127</identifier><identifier>DOI: 10.1186/1423-0127-17-S1-S13</identifier><identifier>PMID: 20804587</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Carassius auratus ; Goldfish ; Membrane Glycoproteins - metabolism ; Membrane Transport Proteins - metabolism ; Radioligand Assay ; Retina - cytology ; Retina - drug effects ; Retina - metabolism ; Review ; Taurine - metabolism ; Zinc Sulfate - pharmacology</subject><ispartof>Journal of biomedical science, 2010-08, Vol.17 Suppl 1 (Suppl 1), p.S13-S13</ispartof><rights>Copyright ©2010 Lima et al; licensee BioMed Central Ltd. 2010 Lima et al; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b499t-d865883dcb23ea7cf5df75b7191795711deb72f28a050ae09945fa5d3735220c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994400/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994400/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20804587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nusetti, Sonia</creatorcontrib><creatorcontrib>Urbina, Mary</creatorcontrib><creatorcontrib>Lima, Lucimey</creatorcontrib><title>Effects of zinc ex vivo on taurine uptake in goldfish retinal cells</title><title>Journal of biomedical science</title><addtitle>J Biomed Sci</addtitle><description>Taurine and zinc exert neurotrophic effects in the central nervous system. Current studies demonstrate that Na+/Cl- dependent neurotransmitter transporters, similar to that of taurine, are modulated by micromolar concentrations of zinc. This study examined the effect of zinc sulfate ex vivo on [3H]taurine transport in goldfish retina.
Isolated cells were incubated in Ringer with zinc (0.1-100 microM). Taurine transport was done with 50 nM [3H]taurine or by isotopic dilution with taurine (0.001-1 mM) and 50 nM [3H]taurine.
Zinc reduced the capacity of taurine transport without changes in affinity, and caused a noncompetitive inhibition of high affinity taurine transport, with an EC50= 0.072 microM. The mechanism by which zinc affects taurine transport is unknown at the present.
There may be a binding site of zinc in the transporter that affects union or translocation of taurine, or possibly the formation of taurine-zinc complexes, rather than free zinc, could affect the operation of the transporter.</description><subject>Animals</subject><subject>Carassius auratus</subject><subject>Goldfish</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Radioligand Assay</subject><subject>Retina - cytology</subject><subject>Retina - drug effects</subject><subject>Retina - metabolism</subject><subject>Review</subject><subject>Taurine - metabolism</subject><subject>Zinc Sulfate - pharmacology</subject><issn>1423-0127</issn><issn>1021-7770</issn><issn>1423-0127</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LAzEQhoMotn78AkFy87SaSTZNchGk-AWCh-o5ZLOJRrebutkt6q93S2tpDyIMZMi88_DyDkInQM4B5OgCcsoyAlRkILIJ9MV20HD9u7vRD9BBSm-EAFdS7KMBJZLkXIohGl9772ybcPT4O9QWu088D_OIY41b0zWhdribtebd4VDjl1iVPqRX3Lg21KbC1lVVOkJ73lTJHa_eQ_R8c_00vsseHm_vx1cPWZEr1WalHHEpWWkLypwR1vPSC14IUCAUFwClKwT1VBrCiXFEqZx7w0smGKeUWHaILpfcWVdMXWld3Tam0rMmTE3zpaMJentSh1f9Euea9qickB5wswQUIf4B2J7YONWLEPUiRA1CT6Av1oPOVk6a-NG51OppSIssTO1il7QaSRiBEOpfpeA5YQQI9Eq2VNomptQ4v_YFRC8O_oeT081M1ju_F2Y_E6CnCQ</recordid><startdate>20100824</startdate><enddate>20100824</enddate><creator>Nusetti, Sonia</creator><creator>Urbina, Mary</creator><creator>Lima, Lucimey</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20100824</creationdate><title>Effects of zinc ex vivo on taurine uptake in goldfish retinal cells</title><author>Nusetti, Sonia ; Urbina, Mary ; Lima, Lucimey</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b499t-d865883dcb23ea7cf5df75b7191795711deb72f28a050ae09945fa5d3735220c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Carassius auratus</topic><topic>Goldfish</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Radioligand Assay</topic><topic>Retina - cytology</topic><topic>Retina - drug effects</topic><topic>Retina - metabolism</topic><topic>Review</topic><topic>Taurine - metabolism</topic><topic>Zinc Sulfate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nusetti, Sonia</creatorcontrib><creatorcontrib>Urbina, Mary</creatorcontrib><creatorcontrib>Lima, Lucimey</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biomedical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nusetti, Sonia</au><au>Urbina, Mary</au><au>Lima, Lucimey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of zinc ex vivo on taurine uptake in goldfish retinal cells</atitle><jtitle>Journal of biomedical science</jtitle><addtitle>J Biomed Sci</addtitle><date>2010-08-24</date><risdate>2010</risdate><volume>17 Suppl 1</volume><issue>Suppl 1</issue><spage>S13</spage><epage>S13</epage><pages>S13-S13</pages><issn>1423-0127</issn><issn>1021-7770</issn><eissn>1423-0127</eissn><abstract>Taurine and zinc exert neurotrophic effects in the central nervous system. Current studies demonstrate that Na+/Cl- dependent neurotransmitter transporters, similar to that of taurine, are modulated by micromolar concentrations of zinc. This study examined the effect of zinc sulfate ex vivo on [3H]taurine transport in goldfish retina.
Isolated cells were incubated in Ringer with zinc (0.1-100 microM). Taurine transport was done with 50 nM [3H]taurine or by isotopic dilution with taurine (0.001-1 mM) and 50 nM [3H]taurine.
Zinc reduced the capacity of taurine transport without changes in affinity, and caused a noncompetitive inhibition of high affinity taurine transport, with an EC50= 0.072 microM. The mechanism by which zinc affects taurine transport is unknown at the present.
There may be a binding site of zinc in the transporter that affects union or translocation of taurine, or possibly the formation of taurine-zinc complexes, rather than free zinc, could affect the operation of the transporter.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>20804587</pmid><doi>10.1186/1423-0127-17-S1-S13</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Springer Nature OA Free Journals; PubMed Central |
| subjects | Animals Carassius auratus Goldfish Membrane Glycoproteins - metabolism Membrane Transport Proteins - metabolism Radioligand Assay Retina - cytology Retina - drug effects Retina - metabolism Review Taurine - metabolism Zinc Sulfate - pharmacology |
| title | Effects of zinc ex vivo on taurine uptake in goldfish retinal cells |
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