Validation of reverse phase protein array for practical screening of potential biomarkers in serum and plasma: Accurate detection of CA19-9 levels in pancreatic cancer

The current study analyzed reverse phase protein arrays (RPPA) as a means to experimentally validate biomarkers in blood samples. One microliter samples of sera (n = 71), and plasma (n = 78) were serially diluted and printed on NC-coated slides. CA19-9 levels from RPPA results were compared with ide...

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Veröffentlicht in:	Proteomics (Weinheim) 2008-08, Vol.8 (15), p.3051-3060
Hauptverfasser:	Grote, Tobias, Siwak, Doris R, Fritsche, Herbert A, Joy, Corwin, Mills, Gordon B, Simeone, Diane, Whitcomb, David C, Logsdon, Craig D
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Biomarker Biomarkers - blood Blood Proteins - analysis CA-19-9 Antigen - blood CA19-9 Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Gastroenterology. Liver. Pancreas. Abdomen Humans Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences Miscellaneous Pancreatic cancer Pancreatic Neoplasms - blood Plasma - chemistry Protein Array Analysis - methods Proteins Proteomics - methods Reproducibility of Results Reverse phase protein array Serum - chemistry Tumors
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