Genetic variations in the SMAD4 gene and gastric cancer susceptibility
To explore the association between mothers against decapentaplegic homolog 4 (SMAD4) gene polymorphisms and gastric cancer risk. Five tagging single nucleotide polymorphisms (tSNPs) in the SMAD4 gene were selected and genotyped in 322 gastric cancer cases and 351 cancer-free controls in a Chinese po...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2010-11, Vol.16 (44), p.5635-5641 |
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| container_title | World journal of gastroenterology : WJG |
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| creator | Wu, Dong-Mei Zhu, Hai-Xia Zhao, Qing-Hong Zhang, Zhi-Zhong Wang, Shi-Zhi Wang, Mei-Lin Gong, Wei-Da Tan, Ming Zhang, Zheng-Dong |
| description | To explore the association between mothers against decapentaplegic homolog 4 (SMAD4) gene polymorphisms and gastric cancer risk.
Five tagging single nucleotide polymorphisms (tSNPs) in the SMAD4 gene were selected and genotyped in 322 gastric cancer cases and 351 cancer-free controls in a Chinese population by using the polymerase chain reaction restriction fragment length polymorphism method. Immunohistochemistry was used to examine SMAD4 protein expression in 10 normal gastric tissues adjacent to tumors.
In the single-locus analysis, two significantly decreased risk polymorphisms for gastric cancer were observed: the SNP3 rs17663887 TC genotype (adjusted odds ratio = 0.38, 95% confidence interval: 0.21-0.71), compared with the wild-type TT genotype and the SNP5 rs12456284 GG genotype (0.31, 0.16-0.60), and with the wild-type AA genotype. In the combined analyses of these two tSNPs, the combined genotypes with 2-3 protective alleles (SNP3 C and SNP5 G allele) had a significantly decreased risk of gastric cancer (0.28, 0.16-0.49) than those with 0-1 protective allele. Furthermore, individuals with 0-1 protective allele had significantly decreased SMAD4 protein expression levels in the normal tissues adjacent to tumors than those with 2-3 protective alleles (P = 0.025).
These results suggest that genetic variants in the SMAD4 gene play a protective role in gastric cancer in a Chinese population. |
| doi_str_mv | 10.3748/wjg.v16.i44.5635 |
| format | Article |
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Five tagging single nucleotide polymorphisms (tSNPs) in the SMAD4 gene were selected and genotyped in 322 gastric cancer cases and 351 cancer-free controls in a Chinese population by using the polymerase chain reaction restriction fragment length polymorphism method. Immunohistochemistry was used to examine SMAD4 protein expression in 10 normal gastric tissues adjacent to tumors.
In the single-locus analysis, two significantly decreased risk polymorphisms for gastric cancer were observed: the SNP3 rs17663887 TC genotype (adjusted odds ratio = 0.38, 95% confidence interval: 0.21-0.71), compared with the wild-type TT genotype and the SNP5 rs12456284 GG genotype (0.31, 0.16-0.60), and with the wild-type AA genotype. In the combined analyses of these two tSNPs, the combined genotypes with 2-3 protective alleles (SNP3 C and SNP5 G allele) had a significantly decreased risk of gastric cancer (0.28, 0.16-0.49) than those with 0-1 protective allele. Furthermore, individuals with 0-1 protective allele had significantly decreased SMAD4 protein expression levels in the normal tissues adjacent to tumors than those with 2-3 protective alleles (P = 0.025).
These results suggest that genetic variants in the SMAD4 gene play a protective role in gastric cancer in a Chinese population.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v16.i44.5635</identifier><identifier>PMID: 21105199</identifier><language>eng</language><publisher>United States: Baishideng Publishing Group Co., Limited</publisher><subject>Asian Continental Ancestry Group - genetics ; Brief ; Case-Control Studies ; Chi-Square Distribution ; China ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Odds Ratio ; Phenotype ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Risk Assessment ; Risk Factors ; Smad4 Protein - analysis ; Smad4 Protein - genetics ; Stomach Neoplasms - chemistry ; Stomach Neoplasms - ethnology ; Stomach Neoplasms - genetics</subject><ispartof>World journal of gastroenterology : WJG, 2010-11, Vol.16 (44), p.5635-5641</ispartof><rights>2010 Baishideng Publishing Group Co., Limited. All rights reserved. 2010</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-7a64faf410d175e18c609e2e047ecd238dd2f1a3d2a9da3e5cdfa9c4d0d1761c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2992684/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2992684/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21105199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Dong-Mei</creatorcontrib><creatorcontrib>Zhu, Hai-Xia</creatorcontrib><creatorcontrib>Zhao, Qing-Hong</creatorcontrib><creatorcontrib>Zhang, Zhi-Zhong</creatorcontrib><creatorcontrib>Wang, Shi-Zhi</creatorcontrib><creatorcontrib>Wang, Mei-Lin</creatorcontrib><creatorcontrib>Gong, Wei-Da</creatorcontrib><creatorcontrib>Tan, Ming</creatorcontrib><creatorcontrib>Zhang, Zheng-Dong</creatorcontrib><title>Genetic variations in the SMAD4 gene and gastric cancer susceptibility</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To explore the association between mothers against decapentaplegic homolog 4 (SMAD4) gene polymorphisms and gastric cancer risk.
Five tagging single nucleotide polymorphisms (tSNPs) in the SMAD4 gene were selected and genotyped in 322 gastric cancer cases and 351 cancer-free controls in a Chinese population by using the polymerase chain reaction restriction fragment length polymorphism method. Immunohistochemistry was used to examine SMAD4 protein expression in 10 normal gastric tissues adjacent to tumors.
In the single-locus analysis, two significantly decreased risk polymorphisms for gastric cancer were observed: the SNP3 rs17663887 TC genotype (adjusted odds ratio = 0.38, 95% confidence interval: 0.21-0.71), compared with the wild-type TT genotype and the SNP5 rs12456284 GG genotype (0.31, 0.16-0.60), and with the wild-type AA genotype. In the combined analyses of these two tSNPs, the combined genotypes with 2-3 protective alleles (SNP3 C and SNP5 G allele) had a significantly decreased risk of gastric cancer (0.28, 0.16-0.49) than those with 0-1 protective allele. Furthermore, individuals with 0-1 protective allele had significantly decreased SMAD4 protein expression levels in the normal tissues adjacent to tumors than those with 2-3 protective alleles (P = 0.025).
These results suggest that genetic variants in the SMAD4 gene play a protective role in gastric cancer in a Chinese population.</description><subject>Asian Continental Ancestry Group - genetics</subject><subject>Brief</subject><subject>Case-Control Studies</subject><subject>Chi-Square Distribution</subject><subject>China</subject><subject>Female</subject><subject>Gene Frequency</subject><subject>Genetic Predisposition to Disease</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Odds Ratio</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Risk Assessment</subject><subject>Risk Factors</subject><subject>Smad4 Protein - analysis</subject><subject>Smad4 Protein - genetics</subject><subject>Stomach Neoplasms - chemistry</subject><subject>Stomach Neoplasms - ethnology</subject><subject>Stomach Neoplasms - genetics</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtPAjEUhRujEUT3rkz_wIx9zaMbE4KCJhgX6rq5tJ2hBGZIW8bw7x2CEl3dxTnfucmH0C0lKS9Eef-1qtOO5qkTIs1ynp2hIWNUJqwU5BwNKSFFIjkrBugqhBUhjPOMXaIBo5RkVMohms5sY6PTuAPvILq2Cdg1OC4tfn8dPwpc9zmGxuAaQvR9UUOjrcdhF7TdRrdwaxf31-iignWwNz93hD6nTx-T52T-NnuZjOeJ5hmPSQG5qKASlBhaZJaWOifSMktEYbVhvDSGVRS4YSANcJtpU4HUwhz6OdV8hB6Ou9vdYmONtk30sFZb7zbg96oFp_4njVuquu0Uk5LlpegHyHFA-zYEb6sTS4k6OFW9U9U7Vb1TdXDaI3d_f56AX4n8G6ygddw</recordid><startdate>20101128</startdate><enddate>20101128</enddate><creator>Wu, Dong-Mei</creator><creator>Zhu, Hai-Xia</creator><creator>Zhao, Qing-Hong</creator><creator>Zhang, Zhi-Zhong</creator><creator>Wang, Shi-Zhi</creator><creator>Wang, Mei-Lin</creator><creator>Gong, Wei-Da</creator><creator>Tan, Ming</creator><creator>Zhang, Zheng-Dong</creator><general>Baishideng Publishing Group Co., Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20101128</creationdate><title>Genetic variations in the SMAD4 gene and gastric cancer susceptibility</title><author>Wu, Dong-Mei ; Zhu, Hai-Xia ; Zhao, Qing-Hong ; Zhang, Zhi-Zhong ; Wang, Shi-Zhi ; Wang, Mei-Lin ; Gong, Wei-Da ; Tan, Ming ; Zhang, Zheng-Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-7a64faf410d175e18c609e2e047ecd238dd2f1a3d2a9da3e5cdfa9c4d0d1761c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Asian Continental Ancestry Group - genetics</topic><topic>Brief</topic><topic>Case-Control Studies</topic><topic>Chi-Square Distribution</topic><topic>China</topic><topic>Female</topic><topic>Gene Frequency</topic><topic>Genetic Predisposition to Disease</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Odds Ratio</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Risk Assessment</topic><topic>Risk Factors</topic><topic>Smad4 Protein - analysis</topic><topic>Smad4 Protein - genetics</topic><topic>Stomach Neoplasms - chemistry</topic><topic>Stomach Neoplasms - ethnology</topic><topic>Stomach Neoplasms - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Wu, Dong-Mei</creatorcontrib><creatorcontrib>Zhu, Hai-Xia</creatorcontrib><creatorcontrib>Zhao, Qing-Hong</creatorcontrib><creatorcontrib>Zhang, Zhi-Zhong</creatorcontrib><creatorcontrib>Wang, Shi-Zhi</creatorcontrib><creatorcontrib>Wang, Mei-Lin</creatorcontrib><creatorcontrib>Gong, Wei-Da</creatorcontrib><creatorcontrib>Tan, Ming</creatorcontrib><creatorcontrib>Zhang, Zheng-Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Dong-Mei</au><au>Zhu, Hai-Xia</au><au>Zhao, Qing-Hong</au><au>Zhang, Zhi-Zhong</au><au>Wang, Shi-Zhi</au><au>Wang, Mei-Lin</au><au>Gong, Wei-Da</au><au>Tan, Ming</au><au>Zhang, Zheng-Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic variations in the SMAD4 gene and gastric cancer susceptibility</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2010-11-28</date><risdate>2010</risdate><volume>16</volume><issue>44</issue><spage>5635</spage><epage>5641</epage><pages>5635-5641</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To explore the association between mothers against decapentaplegic homolog 4 (SMAD4) gene polymorphisms and gastric cancer risk.
Five tagging single nucleotide polymorphisms (tSNPs) in the SMAD4 gene were selected and genotyped in 322 gastric cancer cases and 351 cancer-free controls in a Chinese population by using the polymerase chain reaction restriction fragment length polymorphism method. Immunohistochemistry was used to examine SMAD4 protein expression in 10 normal gastric tissues adjacent to tumors.
In the single-locus analysis, two significantly decreased risk polymorphisms for gastric cancer were observed: the SNP3 rs17663887 TC genotype (adjusted odds ratio = 0.38, 95% confidence interval: 0.21-0.71), compared with the wild-type TT genotype and the SNP5 rs12456284 GG genotype (0.31, 0.16-0.60), and with the wild-type AA genotype. In the combined analyses of these two tSNPs, the combined genotypes with 2-3 protective alleles (SNP3 C and SNP5 G allele) had a significantly decreased risk of gastric cancer (0.28, 0.16-0.49) than those with 0-1 protective allele. Furthermore, individuals with 0-1 protective allele had significantly decreased SMAD4 protein expression levels in the normal tissues adjacent to tumors than those with 2-3 protective alleles (P = 0.025).
These results suggest that genetic variants in the SMAD4 gene play a protective role in gastric cancer in a Chinese population.</abstract><cop>United States</cop><pub>Baishideng Publishing Group Co., Limited</pub><pmid>21105199</pmid><doi>10.3748/wjg.v16.i44.5635</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Asian Continental Ancestry Group - genetics Brief Case-Control Studies Chi-Square Distribution China Female Gene Frequency Genetic Predisposition to Disease Humans Immunohistochemistry Male Middle Aged Odds Ratio Phenotype Polymerase Chain Reaction Polymorphism, Single Nucleotide Risk Assessment Risk Factors Smad4 Protein - analysis Smad4 Protein - genetics Stomach Neoplasms - chemistry Stomach Neoplasms - ethnology Stomach Neoplasms - genetics |
| title | Genetic variations in the SMAD4 gene and gastric cancer susceptibility |
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