Screening compounds against HCV based on MAVS/IFN-β pathway in a replicon model

To develop a sensitive assay for screening compounds against hepatitis C virus (HCV). The proteolytic cleavage of NS3/4A on enhanced yellow fluorescent protein (eYFP)-mitochondrial antiviral signaling protein (MAVS) was examined by reporter enzyme secreted placental alkaline phosphatase (SEAP), whic...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	World journal of gastroenterology : WJG 2010-11, Vol.16 (44), p.5582-5587
Hauptverfasser:	Fu, Qiu-Xia, Wang, Li-Cui, Jia, Shuai-Zheng, Gao, Bo, Zhou, Yong, Du, Juan, Wang, Ying-Li, Wang, Xiao-Hui, Peng, Jian-Chun, Zhan, Lin-Sheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - metabolism Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Antiviral Agents - pharmacology Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosensing Techniques Blotting, Western Cell Line, Tumor Cyclosporine - pharmacology Dose-Response Relationship, Drug Drug Evaluation, Preclinical Genes, Reporter GPI-Linked Proteins - genetics GPI-Linked Proteins - metabolism Hepacivirus - drug effects Hepacivirus - enzymology Hepacivirus - genetics Humans Interferon-alpha - pharmacology Interferon-beta - genetics Isoenzymes - genetics Isoenzymes - metabolism Luminescent Proteins - genetics Luminescent Proteins - metabolism Microscopy, Fluorescence Original Promoter Regions, Genetic Recombinant Fusion Proteins - metabolism Replicon Signal Transduction - drug effects Time Factors Transcriptional Activation Transfection Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!