Microcavitary Hydrogel-Mediating Phase Transfer Cell Culture for Cartilage Tissue Engineering
Hydrogels have been widely used as cell-laden vehicles for therapeutic transplantation in regenerative medicine. Although the advantages of biocompatibility and injectability for in situ grafting have made hydrogel a superior candidate in tissue engineering, there remain challenges in long-term effi...
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Veröffentlicht in: | Tissue engineering. Part A 2010-12, Vol.16 (12), p.3611-3622 |
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creator | Gong, Yihong Su, Kai Lau, Ting Ting Zhou, Ruijie Wang, Dong-An |
description | Hydrogels have been widely used as cell-laden vehicles for therapeutic transplantation in regenerative medicine. Although the advantages of biocompatibility and injectability for
in situ
grafting have made hydrogel a superior candidate in tissue engineering, there remain challenges in long-term efficacy of tissue development using hydrogel, especially when more sophisticated applications are demanded. The major bottleneck lies in environmental constraints for neo-tissue generation in the gel bulk such as proliferation of encapsulated cells (colonies)
per se
and also accommodation of their endogenously produced extracellular matrices. In this study, we endeavor to develop an innovative tissue engineering system to overcome these drawbacks through a novel microcavitary hydrogel (MCG)-based scaffolding technology and a novel phase transfer cell culture (PTCC) strategy to enable phenotypically
bona fide
neo-tissue formation in an injectable artificial graft. For this purpose, microspherical cavities are created in cell-encapsulating hydrogel bulk via a retarded dissolution of coencapsulated gelatin microspheres. Based on proliferation and affinity selection, the encapsulated cell colonies adjacent to the gel-cavity interface will spontaneously outgrow the hydrogel phase and sprout into cavities, enabling neo-tissue islets to fill up the voids and further expand throughout the whole system for full tissue regeneration. The design of MCG-PTCC strategy was elicited from an observation of a spontaneous dynamic outgrowth of chondrocytes from the edge of a cell-laden hydrogel construct over prolonged cultivation—a phenomenon named edge flourish. This MCG-PTCC strategy potentially introduce a new application to hydrogels in the field of regenerative medicine through elevation of its role as a cell vehicle to a three-dimensional transplantable growth-guiding platform for further development of newly generated tissues that better fulfill the demanding criteria of scaffolds in therapeutic tissue regeneration. |
doi_str_mv | 10.1089/ten.tea.2010.0219 |
format | Article |
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in situ
grafting have made hydrogel a superior candidate in tissue engineering, there remain challenges in long-term efficacy of tissue development using hydrogel, especially when more sophisticated applications are demanded. The major bottleneck lies in environmental constraints for neo-tissue generation in the gel bulk such as proliferation of encapsulated cells (colonies)
per se
and also accommodation of their endogenously produced extracellular matrices. In this study, we endeavor to develop an innovative tissue engineering system to overcome these drawbacks through a novel microcavitary hydrogel (MCG)-based scaffolding technology and a novel phase transfer cell culture (PTCC) strategy to enable phenotypically
bona fide
neo-tissue formation in an injectable artificial graft. For this purpose, microspherical cavities are created in cell-encapsulating hydrogel bulk via a retarded dissolution of coencapsulated gelatin microspheres. Based on proliferation and affinity selection, the encapsulated cell colonies adjacent to the gel-cavity interface will spontaneously outgrow the hydrogel phase and sprout into cavities, enabling neo-tissue islets to fill up the voids and further expand throughout the whole system for full tissue regeneration. The design of MCG-PTCC strategy was elicited from an observation of a spontaneous dynamic outgrowth of chondrocytes from the edge of a cell-laden hydrogel construct over prolonged cultivation—a phenomenon named edge flourish. This MCG-PTCC strategy potentially introduce a new application to hydrogels in the field of regenerative medicine through elevation of its role as a cell vehicle to a three-dimensional transplantable growth-guiding platform for further development of newly generated tissues that better fulfill the demanding criteria of scaffolds in therapeutic tissue regeneration.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2010.0219</identifier><identifier>PMID: 20666616</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Biomedical materials ; Cartilage ; Cartilage - cytology ; Cell culture ; Cell growth ; Cell proliferation ; Cells, Cultured ; Genotype & phenotype ; Health aspects ; Hydrogel, Polyethylene Glycol Dimethacrylate ; Hydrogels ; Mice ; Mice, Nude ; Microscopy, Electron, Scanning ; Original ; Original Articles ; Polymers ; Reverse Transcriptase Polymerase Chain Reaction ; Swine ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Tissue engineering. Part A, 2010-12, Vol.16 (12), p.3611-3622</ispartof><rights>2010, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2010 Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2010, Mary Ann Liebert, Inc.</rights><rights>Copyright 2010, Mary Ann Liebert, Inc. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c638t-c572600aa09999f9fbecdd4156f229d5c3f1c2df84f48c6d3763b60593205e423</citedby><cites>FETCH-LOGICAL-c638t-c572600aa09999f9fbecdd4156f229d5c3f1c2df84f48c6d3763b60593205e423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20666616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Yihong</creatorcontrib><creatorcontrib>Su, Kai</creatorcontrib><creatorcontrib>Lau, Ting Ting</creatorcontrib><creatorcontrib>Zhou, Ruijie</creatorcontrib><creatorcontrib>Wang, Dong-An</creatorcontrib><title>Microcavitary Hydrogel-Mediating Phase Transfer Cell Culture for Cartilage Tissue Engineering</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Hydrogels have been widely used as cell-laden vehicles for therapeutic transplantation in regenerative medicine. Although the advantages of biocompatibility and injectability for
in situ
grafting have made hydrogel a superior candidate in tissue engineering, there remain challenges in long-term efficacy of tissue development using hydrogel, especially when more sophisticated applications are demanded. The major bottleneck lies in environmental constraints for neo-tissue generation in the gel bulk such as proliferation of encapsulated cells (colonies)
per se
and also accommodation of their endogenously produced extracellular matrices. In this study, we endeavor to develop an innovative tissue engineering system to overcome these drawbacks through a novel microcavitary hydrogel (MCG)-based scaffolding technology and a novel phase transfer cell culture (PTCC) strategy to enable phenotypically
bona fide
neo-tissue formation in an injectable artificial graft. For this purpose, microspherical cavities are created in cell-encapsulating hydrogel bulk via a retarded dissolution of coencapsulated gelatin microspheres. Based on proliferation and affinity selection, the encapsulated cell colonies adjacent to the gel-cavity interface will spontaneously outgrow the hydrogel phase and sprout into cavities, enabling neo-tissue islets to fill up the voids and further expand throughout the whole system for full tissue regeneration. The design of MCG-PTCC strategy was elicited from an observation of a spontaneous dynamic outgrowth of chondrocytes from the edge of a cell-laden hydrogel construct over prolonged cultivation—a phenomenon named edge flourish. This MCG-PTCC strategy potentially introduce a new application to hydrogels in the field of regenerative medicine through elevation of its role as a cell vehicle to a three-dimensional transplantable growth-guiding platform for further development of newly generated tissues that better fulfill the demanding criteria of scaffolds in therapeutic tissue regeneration.</description><subject>Animals</subject><subject>Biomedical materials</subject><subject>Cartilage</subject><subject>Cartilage - cytology</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cells, Cultured</subject><subject>Genotype & phenotype</subject><subject>Health aspects</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate</subject><subject>Hydrogels</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Microscopy, Electron, Scanning</subject><subject>Original</subject><subject>Original Articles</subject><subject>Polymers</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Swine</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkk1rFTEUhgdRbK3-ADcy2IWrueZr8rERyqVaoUUXFdxIyM2cTFPmJjXJFPrvzXjrxYqgGUKGk-d9mXPmbZqXGK0wkuptgbAqYFYE1QoiWD1qDrGioqO0__p4_87wQfMs52uEOOJCPG0OCOJ1YX7YfLvwNkVrbn0x6a49uxtSHGHqLmDwpvgwtp-vTIb2MpmQHaR2DdPUruepzAlaF2vBpOInM1bG5zxDexpGHwBSFT9vnjgzZXhxfx41X96fXq7PuvNPHz6uT847y6ksne0F4QgZg1RdTrkN2GFguOeOEDX0ljpsyeAkc0xaPlDB6YajXlGCemCEHjXvdr4382YLg4VQkpn0TfLb2pWOxuuHN8Ff6THeaqIUJmgxeHNvkOL3GXLRW59tbdUEiHPWkglGlZL83ySWDFGBUCVf_0FexzmFOoefkFSsFxU63kGjmUD74GL9PrtY6hPCZC-kErhSq79Q9Rlg620M4HytPxDgnaD-3JwTuP0sMNJLdnTNTt1GL9nRS3aq5tXvQ9wrfoWlAmIHLGUTwuRhA6n8h_UPBxjTXg</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Gong, Yihong</creator><creator>Su, Kai</creator><creator>Lau, Ting Ting</creator><creator>Zhou, Ruijie</creator><creator>Wang, Dong-An</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20101201</creationdate><title>Microcavitary Hydrogel-Mediating Phase Transfer Cell Culture for Cartilage Tissue Engineering</title><author>Gong, Yihong ; Su, Kai ; Lau, Ting Ting ; Zhou, Ruijie ; Wang, Dong-An</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c638t-c572600aa09999f9fbecdd4156f229d5c3f1c2df84f48c6d3763b60593205e423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biomedical materials</topic><topic>Cartilage</topic><topic>Cartilage - cytology</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Cells, Cultured</topic><topic>Genotype & phenotype</topic><topic>Health aspects</topic><topic>Hydrogel, Polyethylene Glycol Dimethacrylate</topic><topic>Hydrogels</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Microscopy, Electron, Scanning</topic><topic>Original</topic><topic>Original Articles</topic><topic>Polymers</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Swine</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Yihong</creatorcontrib><creatorcontrib>Su, Kai</creatorcontrib><creatorcontrib>Lau, Ting Ting</creatorcontrib><creatorcontrib>Zhou, Ruijie</creatorcontrib><creatorcontrib>Wang, Dong-An</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Yihong</au><au>Su, Kai</au><au>Lau, Ting Ting</au><au>Zhou, Ruijie</au><au>Wang, Dong-An</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microcavitary Hydrogel-Mediating Phase Transfer Cell Culture for Cartilage Tissue Engineering</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>16</volume><issue>12</issue><spage>3611</spage><epage>3622</epage><pages>3611-3622</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>Hydrogels have been widely used as cell-laden vehicles for therapeutic transplantation in regenerative medicine. Although the advantages of biocompatibility and injectability for
in situ
grafting have made hydrogel a superior candidate in tissue engineering, there remain challenges in long-term efficacy of tissue development using hydrogel, especially when more sophisticated applications are demanded. The major bottleneck lies in environmental constraints for neo-tissue generation in the gel bulk such as proliferation of encapsulated cells (colonies)
per se
and also accommodation of their endogenously produced extracellular matrices. In this study, we endeavor to develop an innovative tissue engineering system to overcome these drawbacks through a novel microcavitary hydrogel (MCG)-based scaffolding technology and a novel phase transfer cell culture (PTCC) strategy to enable phenotypically
bona fide
neo-tissue formation in an injectable artificial graft. For this purpose, microspherical cavities are created in cell-encapsulating hydrogel bulk via a retarded dissolution of coencapsulated gelatin microspheres. Based on proliferation and affinity selection, the encapsulated cell colonies adjacent to the gel-cavity interface will spontaneously outgrow the hydrogel phase and sprout into cavities, enabling neo-tissue islets to fill up the voids and further expand throughout the whole system for full tissue regeneration. The design of MCG-PTCC strategy was elicited from an observation of a spontaneous dynamic outgrowth of chondrocytes from the edge of a cell-laden hydrogel construct over prolonged cultivation—a phenomenon named edge flourish. This MCG-PTCC strategy potentially introduce a new application to hydrogels in the field of regenerative medicine through elevation of its role as a cell vehicle to a three-dimensional transplantable growth-guiding platform for further development of newly generated tissues that better fulfill the demanding criteria of scaffolds in therapeutic tissue regeneration.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>20666616</pmid><doi>10.1089/ten.tea.2010.0219</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biomedical materials Cartilage Cartilage - cytology Cell culture Cell growth Cell proliferation Cells, Cultured Genotype & phenotype Health aspects Hydrogel, Polyethylene Glycol Dimethacrylate Hydrogels Mice Mice, Nude Microscopy, Electron, Scanning Original Original Articles Polymers Reverse Transcriptase Polymerase Chain Reaction Swine Tissue engineering Tissue Engineering - methods |
title | Microcavitary Hydrogel-Mediating Phase Transfer Cell Culture for Cartilage Tissue Engineering |
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