Antibacterial peptides from pig intestine: isolation of a mammalian cecropin
Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatogr...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-12, Vol.86 (23), p.9159-9162 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Lee, J.Y. (University of Stockholm, Stockholm, Sweden) BOman, A Chuanxin, S Andersson, M Jornvall, H Mutt, V Boman, H.G |
| description | Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatography. Sequence analysis showed the anti-Bm factor to be apparently similar to vasoactive intestinal peptide. The anti-Ec factor was found to have a 31-residue sequence that was cecropin-like. It was named cecropin P1 and its structure was confirmed by solid-phase synthesis. Synthetic cecropin P1 with and without C-terminal amide was assayed on eight different bacteria. Mobility comparison between synthetic and natural cecropin P1 indicates that the natural peptide has a free C-terminal carboxyl group. |
| doi_str_mv | 10.1073/pnas.86.23.9159 |
| format | Article |
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(University of Stockholm, Stockholm, Sweden) ; BOman, A ; Chuanxin, S ; Andersson, M ; Jornvall, H ; Mutt, V ; Boman, H.G</creator><creatorcontrib>Lee, J.Y. (University of Stockholm, Stockholm, Sweden) ; BOman, A ; Chuanxin, S ; Andersson, M ; Jornvall, H ; Mutt, V ; Boman, H.G</creatorcontrib><description>Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatography. Sequence analysis showed the anti-Bm factor to be apparently similar to vasoactive intestinal peptide. The anti-Ec factor was found to have a 31-residue sequence that was cecropin-like. It was named cecropin P1 and its structure was confirmed by solid-phase synthesis. Synthetic cecropin P1 with and without C-terminal amide was assayed on eight different bacteria. Mobility comparison between synthetic and natural cecropin P1 indicates that the natural peptide has a free C-terminal carboxyl group.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.23.9159</identifier><identifier>PMID: 2512577</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>ACCION DE LOS PLAGUICIDAS ; ACTION DES PESTICIDES ; Amino Acid Sequence ; Aminoacids, peptides. Hormones. Neuropeptides ; Analytical, structural and metabolic biochemistry ; Animals ; Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Antibacterials ; ANTIBIOTICOS ; ANTIBIOTICS ; ANTIBIOTIQUE ; BACILLUS ; BACILLUS MEGATERIUM ; Bacillus megaterium - drug effects ; Bacteria ; BACTERICIDAS ; BACTERICIDE ; BACTERICIDES ; Biochemistry ; Biological and medical sciences ; Carboxyl compounds ; cercropin P1 ; CERDO ; Elution ; ENFERMEDADES INTESTINALES ; ESCHERICHIA ; ESCHERICHIA COLI ; Escherichia coli - drug effects ; Ethanol ; FLORA MICROBIANA ; FLORE MICROBIENNE ; Fundamental and applied biological sciences. Psychology ; IMMUNITE ; IMMUNITY ; INMUNIDAD ; INTESTIN ; INTESTINAL DISEASES ; Intestine, Small - analysis ; INTESTINES ; INTESTINOS ; MALADIE INTESTINALE ; MICROBIAL FLORA ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Muscle, Smooth - analysis ; PEPTIDE ; PEPTIDES ; Peptides - isolation & purification ; Peptides - pharmacology ; PEPTIDOS ; PESTICIDE ACTIONS ; PORCIN ; Precipitates ; Proteins ; Sequence Homology, Nucleic Acid ; Small intestine ; SWINE</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-12, Vol.86 (23), p.9159-9162</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c606t-d19f988801df45836e1371a18dfd4a73cdf6e7515971b924ff622b78c461b9c23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/23.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/35039$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/35039$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6732398$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2512577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, J.Y. (University of Stockholm, Stockholm, Sweden)</creatorcontrib><creatorcontrib>BOman, A</creatorcontrib><creatorcontrib>Chuanxin, S</creatorcontrib><creatorcontrib>Andersson, M</creatorcontrib><creatorcontrib>Jornvall, H</creatorcontrib><creatorcontrib>Mutt, V</creatorcontrib><creatorcontrib>Boman, H.G</creatorcontrib><title>Antibacterial peptides from pig intestine: isolation of a mammalian cecropin</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatography. Sequence analysis showed the anti-Bm factor to be apparently similar to vasoactive intestinal peptide. The anti-Ec factor was found to have a 31-residue sequence that was cecropin-like. It was named cecropin P1 and its structure was confirmed by solid-phase synthesis. Synthetic cecropin P1 with and without C-terminal amide was assayed on eight different bacteria. Mobility comparison between synthetic and natural cecropin P1 indicates that the natural peptide has a free C-terminal carboxyl group.</description><subject>ACCION DE LOS PLAGUICIDAS</subject><subject>ACTION DES PESTICIDES</subject><subject>Amino Acid Sequence</subject><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterials</subject><subject>ANTIBIOTICOS</subject><subject>ANTIBIOTICS</subject><subject>ANTIBIOTIQUE</subject><subject>BACILLUS</subject><subject>BACILLUS MEGATERIUM</subject><subject>Bacillus megaterium - drug effects</subject><subject>Bacteria</subject><subject>BACTERICIDAS</subject><subject>BACTERICIDE</subject><subject>BACTERICIDES</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carboxyl compounds</subject><subject>cercropin P1</subject><subject>CERDO</subject><subject>Elution</subject><subject>ENFERMEDADES INTESTINALES</subject><subject>ESCHERICHIA</subject><subject>ESCHERICHIA COLI</subject><subject>Escherichia coli - drug effects</subject><subject>Ethanol</subject><subject>FLORA MICROBIANA</subject><subject>FLORE MICROBIENNE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IMMUNITE</subject><subject>IMMUNITY</subject><subject>INMUNIDAD</subject><subject>INTESTIN</subject><subject>INTESTINAL DISEASES</subject><subject>Intestine, Small - analysis</subject><subject>INTESTINES</subject><subject>INTESTINOS</subject><subject>MALADIE INTESTINALE</subject><subject>MICROBIAL FLORA</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Smooth - analysis</subject><subject>PEPTIDE</subject><subject>PEPTIDES</subject><subject>Peptides - isolation & purification</subject><subject>Peptides - pharmacology</subject><subject>PEPTIDOS</subject><subject>PESTICIDE ACTIONS</subject><subject>PORCIN</subject><subject>Precipitates</subject><subject>Proteins</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Small intestine</subject><subject>SWINE</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoYzu6FgSlFqKr6smj8hpwMQy-oMGFzjqkU0mbIZWUSVr035umy3bc6CqE852be3IAeIrgGkFOLuaoy1qwNSZriai8B1YIStSzQcL7YAUh5r0Y8PAQPCrlFkIoqYBn4AxThCnnK7C5itVvtak2ex262c7Vj7Z0Lqepm_2u87HaUn20l50vKejqU-yS63Q36WnSwevYGWtymn18DB44HYp9spzn4Obd2y_XH_rNp_cfr682vWGQ1X5E0kkhBESjG6ggzCLCkUZidOOgOTGjY5bTFoejrcSDcwzjLRdmYO1uMDkHb45z5_12sqOxsWYd1Jz9pPNPlbRXfyvRf1W79F1hKQZKmv_V4s_p277FU5Mvxoago037orhs-1DM_wsiShiUhDXw4gi2jyglW3daBkF1KEodilKCKUzUoajmeH43w4lfmmn6y0XXxejgso7GlxPGOMFEioa9XrDD_N_qn3eU24dQ7Y_ayBf_JBvw7AjclpryiSAUkjui00npXW6r3HyWECM6CPILsrTFuw</recordid><startdate>19891201</startdate><enddate>19891201</enddate><creator>Lee, J.Y. (University of Stockholm, Stockholm, Sweden)</creator><creator>BOman, A</creator><creator>Chuanxin, S</creator><creator>Andersson, M</creator><creator>Jornvall, H</creator><creator>Mutt, V</creator><creator>Boman, H.G</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19891201</creationdate><title>Antibacterial peptides from pig intestine: isolation of a mammalian cecropin</title><author>Lee, J.Y. (University of Stockholm, Stockholm, Sweden) ; BOman, A ; Chuanxin, S ; Andersson, M ; Jornvall, H ; Mutt, V ; Boman, H.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c606t-d19f988801df45836e1371a18dfd4a73cdf6e7515971b924ff622b78c461b9c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>ACCION DE LOS PLAGUICIDAS</topic><topic>ACTION DES PESTICIDES</topic><topic>Amino Acid Sequence</topic><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterials</topic><topic>ANTIBIOTICOS</topic><topic>ANTIBIOTICS</topic><topic>ANTIBIOTIQUE</topic><topic>BACILLUS</topic><topic>BACILLUS MEGATERIUM</topic><topic>Bacillus megaterium - drug effects</topic><topic>Bacteria</topic><topic>BACTERICIDAS</topic><topic>BACTERICIDE</topic><topic>BACTERICIDES</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carboxyl compounds</topic><topic>cercropin P1</topic><topic>CERDO</topic><topic>Elution</topic><topic>ENFERMEDADES INTESTINALES</topic><topic>ESCHERICHIA</topic><topic>ESCHERICHIA COLI</topic><topic>Escherichia coli - drug effects</topic><topic>Ethanol</topic><topic>FLORA MICROBIANA</topic><topic>FLORE MICROBIENNE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>IMMUNITE</topic><topic>IMMUNITY</topic><topic>INMUNIDAD</topic><topic>INTESTIN</topic><topic>INTESTINAL DISEASES</topic><topic>Intestine, Small - analysis</topic><topic>INTESTINES</topic><topic>INTESTINOS</topic><topic>MALADIE INTESTINALE</topic><topic>MICROBIAL FLORA</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Smooth - analysis</topic><topic>PEPTIDE</topic><topic>PEPTIDES</topic><topic>Peptides - isolation & purification</topic><topic>Peptides - pharmacology</topic><topic>PEPTIDOS</topic><topic>PESTICIDE ACTIONS</topic><topic>PORCIN</topic><topic>Precipitates</topic><topic>Proteins</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Small intestine</topic><topic>SWINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, J.Y. (University of Stockholm, Stockholm, Sweden)</creatorcontrib><creatorcontrib>BOman, A</creatorcontrib><creatorcontrib>Chuanxin, S</creatorcontrib><creatorcontrib>Andersson, M</creatorcontrib><creatorcontrib>Jornvall, H</creatorcontrib><creatorcontrib>Mutt, V</creatorcontrib><creatorcontrib>Boman, H.G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, J.Y. (University of Stockholm, Stockholm, Sweden)</au><au>BOman, A</au><au>Chuanxin, S</au><au>Andersson, M</au><au>Jornvall, H</au><au>Mutt, V</au><au>Boman, H.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial peptides from pig intestine: isolation of a mammalian cecropin</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-12-01</date><risdate>1989</risdate><volume>86</volume><issue>23</issue><spage>9159</spage><epage>9162</epage><pages>9159-9162</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatography. Sequence analysis showed the anti-Bm factor to be apparently similar to vasoactive intestinal peptide. The anti-Ec factor was found to have a 31-residue sequence that was cecropin-like. It was named cecropin P1 and its structure was confirmed by solid-phase synthesis. Synthetic cecropin P1 with and without C-terminal amide was assayed on eight different bacteria. Mobility comparison between synthetic and natural cecropin P1 indicates that the natural peptide has a free C-terminal carboxyl group.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2512577</pmid><doi>10.1073/pnas.86.23.9159</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1989-12, Vol.86 (23), p.9159-9162 |
| issn | 0027-8424 1091-6490 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | ACCION DE LOS PLAGUICIDAS ACTION DES PESTICIDES Amino Acid Sequence Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antibacterials ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE BACILLUS BACILLUS MEGATERIUM Bacillus megaterium - drug effects Bacteria BACTERICIDAS BACTERICIDE BACTERICIDES Biochemistry Biological and medical sciences Carboxyl compounds cercropin P1 CERDO Elution ENFERMEDADES INTESTINALES ESCHERICHIA ESCHERICHIA COLI Escherichia coli - drug effects Ethanol FLORA MICROBIANA FLORE MICROBIENNE Fundamental and applied biological sciences. Psychology IMMUNITE IMMUNITY INMUNIDAD INTESTIN INTESTINAL DISEASES Intestine, Small - analysis INTESTINES INTESTINOS MALADIE INTESTINALE MICROBIAL FLORA Microbial Sensitivity Tests Molecular Sequence Data Muscle, Smooth - analysis PEPTIDE PEPTIDES Peptides - isolation & purification Peptides - pharmacology PEPTIDOS PESTICIDE ACTIONS PORCIN Precipitates Proteins Sequence Homology, Nucleic Acid Small intestine SWINE |
| title | Antibacterial peptides from pig intestine: isolation of a mammalian cecropin |
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