Cloning and Functional Expression of a Human Pancreatic Islet Glucose-Transporter cDNA
Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-Km, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isola...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-11, Vol.86 (22), p.8688-8692 |
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| creator | Permutt, M. Alan Koranyi, Laszlo Keller, Konrad Lacy, Paul E. Scharp, David W. Mueckler, Mike |
| description | Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-Km, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. We isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus. |
| doi_str_mv | 10.1073/pnas.86.22.8688 |
| format | Article |
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Alan ; Koranyi, Laszlo ; Keller, Konrad ; Lacy, Paul E. ; Scharp, David W. ; Mueckler, Mike</creator><creatorcontrib>Permutt, M. Alan ; Koranyi, Laszlo ; Keller, Konrad ; Lacy, Paul E. ; Scharp, David W. ; Mueckler, Mike</creatorcontrib><description>Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-Km, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. We isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.22.8688</identifier><identifier>PMID: 2479026</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>ALDEHYDES ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Biochemistry ; Biological and medical sciences ; Blotting, Northern ; BODY ; CARBOHYDRATES ; CDNA libraries ; Cell Line ; CLONING ; Cloning, Molecular ; Complementary DNA ; DAYS LIVING RADIOISOTOPES ; Diabetes mellitus ; DIGESTIVE SYSTEM ; DNA ; DNA - genetics ; DNA HYBRIDIZATION ; DNA-CLONING ; ELECTROPHORESIS ; ENDOCRINE GLANDS ; Facilitative glucose transport proteins ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Library ; GENE REGULATION ; Genes ; Genes. Genome ; GLANDS ; GLUCOSE ; HEXOSES ; Humans ; HYBRIDIZATION ; HYDROGEN COMPOUNDS ; Islets of Langerhans ; Islets of Langerhans - metabolism ; ISOTOPES ; LIGHT NUCLEI ; LIVER ; MAMMALS ; MAN ; MEMBRANE PROTEINS ; MEMBRANE TRANSPORT ; MESSENGER-RNA ; Molecular and cellular biology ; Molecular genetics ; Monosaccharide Transport Proteins - genetics ; MONOSACCHARIDES ; NUCLEI ; NUCLEIC ACIDS ; ODD-ODD NUCLEI ; Oocytes ; Oocytes - metabolism ; ORGANIC COMPOUNDS ; ORGANS ; PANCREAS ; PHOSPHORUS 32 ; PHOSPHORUS ISOTOPES ; PRIMATES ; PROTEINS ; RADIOISOTOPES ; RECOMBINANT DNA ; Restriction Mapping ; RNA ; RNA - genetics ; RNA - isolation & purification ; SACCHARIDES ; TRANSCRIPTION ; TRITIUM COMPOUNDS ; VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques ; Xenopus</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-11, Vol.86 (22), p.8688-8692</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-13565f0eb50e98a27542b45dba0773507883b2b77233411c238d338cf3df0a5d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/22.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/34941$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/34941$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19368402$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2479026$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6906544$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Permutt, M. Alan</creatorcontrib><creatorcontrib>Koranyi, Laszlo</creatorcontrib><creatorcontrib>Keller, Konrad</creatorcontrib><creatorcontrib>Lacy, Paul E.</creatorcontrib><creatorcontrib>Scharp, David W.</creatorcontrib><creatorcontrib>Mueckler, Mike</creatorcontrib><title>Cloning and Functional Expression of a Human Pancreatic Islet Glucose-Transporter cDNA</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-Km, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. We isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus.</description><subject>ALDEHYDES</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>BODY</subject><subject>CARBOHYDRATES</subject><subject>CDNA libraries</subject><subject>Cell Line</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>Diabetes mellitus</subject><subject>DIGESTIVE SYSTEM</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA-CLONING</subject><subject>ELECTROPHORESIS</subject><subject>ENDOCRINE GLANDS</subject><subject>Facilitative glucose transport proteins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Library</subject><subject>GENE REGULATION</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>GLANDS</subject><subject>GLUCOSE</subject><subject>HEXOSES</subject><subject>Humans</subject><subject>HYBRIDIZATION</subject><subject>HYDROGEN COMPOUNDS</subject><subject>Islets of Langerhans</subject><subject>Islets of Langerhans - metabolism</subject><subject>ISOTOPES</subject><subject>LIGHT NUCLEI</subject><subject>LIVER</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>MEMBRANE PROTEINS</subject><subject>MEMBRANE TRANSPORT</subject><subject>MESSENGER-RNA</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Monosaccharide Transport Proteins - genetics</subject><subject>MONOSACCHARIDES</subject><subject>NUCLEI</subject><subject>NUCLEIC ACIDS</subject><subject>ODD-ODD NUCLEI</subject><subject>Oocytes</subject><subject>Oocytes - metabolism</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PANCREAS</subject><subject>PHOSPHORUS 32</subject><subject>PHOSPHORUS ISOTOPES</subject><subject>PRIMATES</subject><subject>PROTEINS</subject><subject>RADIOISOTOPES</subject><subject>RECOMBINANT DNA</subject><subject>Restriction Mapping</subject><subject>RNA</subject><subject>RNA - genetics</subject><subject>RNA - isolation & purification</subject><subject>SACCHARIDES</subject><subject>TRANSCRIPTION</subject><subject>TRITIUM COMPOUNDS</subject><subject>VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</subject><subject>Xenopus</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFDEYxgdR6lo9C4ISBPU023fyMUkOHsraLyjqoXoNmUymnZJNpklG6n9vll1Xe9FLQnh-z_vk5amqlw0sG-DkaPI6LUW7xLicQjyqFg3Ipm6phMfVAgDzWlBMn1bPUroFAMkEHFQHmHIJuF1U31cu-NFfI-17dDp7k8fgtUMn91O0KZUHCgPS6Hxea4--am-i1Xk06CI5m9GZm01Itr6K2qcpxGwjMp8-Hz-vngzaJftidx9W305Prlbn9eWXs4vV8WVtGJW5bghr2QC2Y2Cl0JgzijvK-k4D54QBF4J0uOMcE0KbxmAiekKEGUg_gGY9Oaw-budOc7e2vbE-R-3UFMe1jj9V0KN6qPjxRl2HHwpLQRgp_rdbf0h5VMmM2ZobE7y3JqtWQssoLdD7XUgMd7NNWa3HZKxz2tswJ8UlASCt_C_YsLIFxryAR1vQxJBStMP-xw2oTa9q06sSrcJYbXotjtd_L7rnd0UW_d1O18loN5Q-zJj-jJWkFRRw4d7suE3Ab_lB0Id_AmqYncv2Phfy1Za8TTnEPUqopA35Bfq8y-M</recordid><startdate>19891101</startdate><enddate>19891101</enddate><creator>Permutt, M. Alan</creator><creator>Koranyi, Laszlo</creator><creator>Keller, Konrad</creator><creator>Lacy, Paul E.</creator><creator>Scharp, David W.</creator><creator>Mueckler, Mike</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19891101</creationdate><title>Cloning and Functional Expression of a Human Pancreatic Islet Glucose-Transporter cDNA</title><author>Permutt, M. Alan ; Koranyi, Laszlo ; Keller, Konrad ; Lacy, Paul E. ; Scharp, David W. ; Mueckler, Mike</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-13565f0eb50e98a27542b45dba0773507883b2b77233411c238d338cf3df0a5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>ALDEHYDES</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>BODY</topic><topic>CARBOHYDRATES</topic><topic>CDNA libraries</topic><topic>Cell Line</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>Diabetes mellitus</topic><topic>DIGESTIVE SYSTEM</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA-CLONING</topic><topic>ELECTROPHORESIS</topic><topic>ENDOCRINE GLANDS</topic><topic>Facilitative glucose transport proteins</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Library</topic><topic>GENE REGULATION</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>GLANDS</topic><topic>GLUCOSE</topic><topic>HEXOSES</topic><topic>Humans</topic><topic>HYBRIDIZATION</topic><topic>HYDROGEN COMPOUNDS</topic><topic>Islets of Langerhans</topic><topic>Islets of Langerhans - metabolism</topic><topic>ISOTOPES</topic><topic>LIGHT NUCLEI</topic><topic>LIVER</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>MEMBRANE PROTEINS</topic><topic>MEMBRANE TRANSPORT</topic><topic>MESSENGER-RNA</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Monosaccharide Transport Proteins - genetics</topic><topic>MONOSACCHARIDES</topic><topic>NUCLEI</topic><topic>NUCLEIC ACIDS</topic><topic>ODD-ODD NUCLEI</topic><topic>Oocytes</topic><topic>Oocytes - metabolism</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PANCREAS</topic><topic>PHOSPHORUS 32</topic><topic>PHOSPHORUS ISOTOPES</topic><topic>PRIMATES</topic><topic>PROTEINS</topic><topic>RADIOISOTOPES</topic><topic>RECOMBINANT DNA</topic><topic>Restriction Mapping</topic><topic>RNA</topic><topic>RNA - genetics</topic><topic>RNA - isolation & purification</topic><topic>SACCHARIDES</topic><topic>TRANSCRIPTION</topic><topic>TRITIUM COMPOUNDS</topic><topic>VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Permutt, M. Alan</creatorcontrib><creatorcontrib>Koranyi, Laszlo</creatorcontrib><creatorcontrib>Keller, Konrad</creatorcontrib><creatorcontrib>Lacy, Paul E.</creatorcontrib><creatorcontrib>Scharp, David W.</creatorcontrib><creatorcontrib>Mueckler, Mike</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Permutt, M. Alan</au><au>Koranyi, Laszlo</au><au>Keller, Konrad</au><au>Lacy, Paul E.</au><au>Scharp, David W.</au><au>Mueckler, Mike</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Functional Expression of a Human Pancreatic Islet Glucose-Transporter cDNA</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-11-01</date><risdate>1989</risdate><volume>86</volume><issue>22</issue><spage>8688</spage><epage>8692</epage><pages>8688-8692</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-Km, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. We isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2479026</pmid><doi>10.1073/pnas.86.22.8688</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1989-11, Vol.86 (22), p.8688-8692 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | ALDEHYDES ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biochemistry Biological and medical sciences Blotting, Northern BODY CARBOHYDRATES CDNA libraries Cell Line CLONING Cloning, Molecular Complementary DNA DAYS LIVING RADIOISOTOPES Diabetes mellitus DIGESTIVE SYSTEM DNA DNA - genetics DNA HYBRIDIZATION DNA-CLONING ELECTROPHORESIS ENDOCRINE GLANDS Facilitative glucose transport proteins Female Fundamental and applied biological sciences. Psychology Gene Expression Gene Library GENE REGULATION Genes Genes. Genome GLANDS GLUCOSE HEXOSES Humans HYBRIDIZATION HYDROGEN COMPOUNDS Islets of Langerhans Islets of Langerhans - metabolism ISOTOPES LIGHT NUCLEI LIVER MAMMALS MAN MEMBRANE PROTEINS MEMBRANE TRANSPORT MESSENGER-RNA Molecular and cellular biology Molecular genetics Monosaccharide Transport Proteins - genetics MONOSACCHARIDES NUCLEI NUCLEIC ACIDS ODD-ODD NUCLEI Oocytes Oocytes - metabolism ORGANIC COMPOUNDS ORGANS PANCREAS PHOSPHORUS 32 PHOSPHORUS ISOTOPES PRIMATES PROTEINS RADIOISOTOPES RECOMBINANT DNA Restriction Mapping RNA RNA - genetics RNA - isolation & purification SACCHARIDES TRANSCRIPTION TRITIUM COMPOUNDS VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques Xenopus |
| title | Cloning and Functional Expression of a Human Pancreatic Islet Glucose-Transporter cDNA |
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