Transcriptional Regulatory Regions of Testis-Specific PGK2 Defined in Transgenic Mice
The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is expressed only in meiotic and haploid male germ cells. Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and dev...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-11, Vol.86 (21), p.8437-8441 |
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| creator | Robinson, Murray O. McCarrey, John R. Simon, Melvin I. |
| description | The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is expressed only in meiotic and haploid male germ cells. Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and developmentally regulated manner. To determine the nature and location of sequences controlling this expression, transgenic mice with various lengths of the human PGK2 5′ region fused to the chloramphenicol acetyltransferase (CAT) gene were analyzed for expression. A 323-base-pair region 5′ to the coding region was found to contain information essential for both tissue-specific and developmentally regulated expression of the CAT reporter gene. Transgenic mice containing a PGK2/luciferase-coding constrct were compared with mice containing an equivalent CAT construct. Luciferase gene expression was also testis-specific and was more sensitive than CAT gene expression, but otherwise regulation of the two reporter genes was similar in the germ cells of transgenic mice. Translation of both PGK2/CAT and PGK2/luciferase fusion genes was seen concurrently with the first detectable transcripts. |
| doi_str_mv | 10.1073/pnas.86.21.8437 |
| format | Article |
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Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and developmentally regulated manner. To determine the nature and location of sequences controlling this expression, transgenic mice with various lengths of the human PGK2 5′ region fused to the chloramphenicol acetyltransferase (CAT) gene were analyzed for expression. A 323-base-pair region 5′ to the coding region was found to contain information essential for both tissue-specific and developmentally regulated expression of the CAT reporter gene. Transgenic mice containing a PGK2/luciferase-coding constrct were compared with mice containing an equivalent CAT construct. Luciferase gene expression was also testis-specific and was more sensitive than CAT gene expression, but otherwise regulation of the two reporter genes was similar in the germ cells of transgenic mice. Translation of both PGK2/CAT and PGK2/luciferase fusion genes was seen concurrently with the first detectable transcripts.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.21.8437</identifier><identifier>PMID: 2813402</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Chloramphenicol O-Acetyltransferase - genetics ; Chloramphenicol O-Acetyltransferase - metabolism ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes ; Haploidy ; Humans ; Isoenzymes - genetics ; Luciferases - genetics ; Luciferases - metabolism ; Male ; Mice ; Mice, Transgenic ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Molecules ; Phosphoglycerate Kinase - genetics ; Promoter regions ; Regulatory Sequences, Nucleic Acid ; RNA ; Testes ; Testis - enzymology ; Transcription, Genetic ; Transgenes ; Transgenic animals</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-11, Vol.86 (21), p.8437-8441</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-73c7fd77ce460ced403078be5f31389f0805cf55907a581ba8cf1c575fe65b0f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/21.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/34886$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/34886$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6850932$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2813402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robinson, Murray O.</creatorcontrib><creatorcontrib>McCarrey, John R.</creatorcontrib><creatorcontrib>Simon, Melvin I.</creatorcontrib><title>Transcriptional Regulatory Regions of Testis-Specific PGK2 Defined in Transgenic Mice</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is expressed only in meiotic and haploid male germ cells. Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and developmentally regulated manner. To determine the nature and location of sequences controlling this expression, transgenic mice with various lengths of the human PGK2 5′ region fused to the chloramphenicol acetyltransferase (CAT) gene were analyzed for expression. A 323-base-pair region 5′ to the coding region was found to contain information essential for both tissue-specific and developmentally regulated expression of the CAT reporter gene. Transgenic mice containing a PGK2/luciferase-coding constrct were compared with mice containing an equivalent CAT construct. Luciferase gene expression was also testis-specific and was more sensitive than CAT gene expression, but otherwise regulation of the two reporter genes was similar in the germ cells of transgenic mice. Translation of both PGK2/CAT and PGK2/luciferase fusion genes was seen concurrently with the first detectable transcripts.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Haploidy</subject><subject>Humans</subject><subject>Isoenzymes - genetics</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Molecules</subject><subject>Phosphoglycerate Kinase - genetics</subject><subject>Promoter regions</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>RNA</subject><subject>Testes</subject><subject>Testis - enzymology</subject><subject>Transcription, Genetic</subject><subject>Transgenes</subject><subject>Transgenic animals</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9vFCEUx4nR1G31bGKimYPR02wfvwY4eDBVq7FGo9szYVlYaVhmHGYa-9_LdMdVL3qC8P18H7z3BaFHGJYYBD3tkslL2SwJXkpGxR20wKBw3TAFd9ECgIhaMsLuo-OcrwBAcQlH6IhITBmQBbpc9SZl24duCG0ysfritmM0Q9vfTNtylqvWVyuXh5Drr52zwQdbfT7_QKrXzofkNlVI1W2VrUtF-hise4DueROzezivJ-jy7ZvV2bv64tP5-7NXF7XlUgy1oFb4jRDWsQas2zCgIOTacU8xlcqDBG495wqE4RKvjbQeWy64dw1fg6cn6OW-bjeud25jXRp6E3XXh53pb3Rrgv5bSeGb3rbXmihJlCj-57O_b7-PpUe9C9m6GE1y7Zi1UBQYk_K_IOaMM3wLnu5B27c5984fHoNBT4npKTEtG02wnhIrjid_9nDg54iK_mzWTbYm-jJpG_IBayQHRSfsxYxN9X-pv-_RfoxxcD-GQj79J1mAx3vgKpd_cCBoGURDfwKjd8Cg</recordid><startdate>19891101</startdate><enddate>19891101</enddate><creator>Robinson, Murray O.</creator><creator>McCarrey, John R.</creator><creator>Simon, Melvin I.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19891101</creationdate><title>Transcriptional Regulatory Regions of Testis-Specific PGK2 Defined in Transgenic Mice</title><author>Robinson, Murray O. ; McCarrey, John R. ; Simon, Melvin I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c587t-73c7fd77ce460ced403078be5f31389f0805cf55907a581ba8cf1c575fe65b0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Haploidy</topic><topic>Humans</topic><topic>Isoenzymes - genetics</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Molecules</topic><topic>Phosphoglycerate Kinase - genetics</topic><topic>Promoter regions</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>RNA</topic><topic>Testes</topic><topic>Testis - enzymology</topic><topic>Transcription, Genetic</topic><topic>Transgenes</topic><topic>Transgenic animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robinson, Murray O.</creatorcontrib><creatorcontrib>McCarrey, John R.</creatorcontrib><creatorcontrib>Simon, Melvin I.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robinson, Murray O.</au><au>McCarrey, John R.</au><au>Simon, Melvin I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional Regulatory Regions of Testis-Specific PGK2 Defined in Transgenic Mice</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-11-01</date><risdate>1989</risdate><volume>86</volume><issue>21</issue><spage>8437</spage><epage>8441</epage><pages>8437-8441</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is expressed only in meiotic and haploid male germ cells. Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and developmentally regulated manner. To determine the nature and location of sequences controlling this expression, transgenic mice with various lengths of the human PGK2 5′ region fused to the chloramphenicol acetyltransferase (CAT) gene were analyzed for expression. A 323-base-pair region 5′ to the coding region was found to contain information essential for both tissue-specific and developmentally regulated expression of the CAT reporter gene. Transgenic mice containing a PGK2/luciferase-coding constrct were compared with mice containing an equivalent CAT construct. Luciferase gene expression was also testis-specific and was more sensitive than CAT gene expression, but otherwise regulation of the two reporter genes was similar in the germ cells of transgenic mice. Translation of both PGK2/CAT and PGK2/luciferase fusion genes was seen concurrently with the first detectable transcripts.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2813402</pmid><doi>10.1073/pnas.86.21.8437</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1989-11, Vol.86 (21), p.8437-8441 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Base Sequence Biological and medical sciences Chloramphenicol O-Acetyltransferase - genetics Chloramphenicol O-Acetyltransferase - metabolism Enzymes Fundamental and applied biological sciences. Psychology Gene Expression Genes Haploidy Humans Isoenzymes - genetics Luciferases - genetics Luciferases - metabolism Male Mice Mice, Transgenic Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecules Phosphoglycerate Kinase - genetics Promoter regions Regulatory Sequences, Nucleic Acid RNA Testes Testis - enzymology Transcription, Genetic Transgenes Transgenic animals |
| title | Transcriptional Regulatory Regions of Testis-Specific PGK2 Defined in Transgenic Mice |
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