The Role of 5-HT3 and 5-HT4 Receptors in the Adaptive Mechanism of Colonic Transit Following the Parasympathetic Denervation in Rats
Background Clinical studies show that disturbed colonic motility induced by extrinsic nerves damage is restored over time. We studied whether 5-HT3 and 5HT4 receptors are involved in mediating the adaptive mechanisms following parasympathetic denervation. Methods Parasympathetic denervation of the e...
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| creator | Tong, Weidong, M.D Kamiyama, Yoichi, M.D., Ph.D Ridolfi, Tim J., M.D Zietlow, Aaron, B.S Zheng, Jun, M.D., Ph.D Kosinski, Lauren, M.D Ludwig, Kirk, M.D Takahashi, Toku, M.D., Ph.D |
| description | Background Clinical studies show that disturbed colonic motility induced by extrinsic nerves damage is restored over time. We studied whether 5-HT3 and 5HT4 receptors are involved in mediating the adaptive mechanisms following parasympathetic denervation. Methods Parasympathetic denervation of the entire colon was achieved by bilateral pelvic nerve transection and truncal vagotomy in rats. Colonic transit was measured by calculating the geometric center (GC) of51 Cr distribution. Expression of 5-HT3 and 5HT4 receptor mRNA was determined by real time RT-PCR. Results Parasympathetic denervation caused a significant delay in colonic transit (GC = 4.36) at postoperative day (POD) 1, compared with sham operation (GC = 6.31). Delayed transit was gradually restored by POD 7 (GC = 5.99) after the denervation. Restored colonic transit was antagonized by the administration of 5-HT3 and 5HT4 receptors antagonists at POD 7. 5-HT3 and 5HT4 receptors mRNA expression were significantly increased in the mucosal/submucosal layer at POD 3 or POD 7, whereas no significant difference was observed in the longitudinal muscle layers adherent with the myenteric plexus (LMMP). Conclusions It is suggested that up-regulation of 5-HT3 and 5-HT4 receptors expression in the mucosal/submucosal layer is involved to restore the delayed transit after the parasympathetic denervation in rats. |
| doi_str_mv | 10.1016/j.jss.2010.05.002 |
| format | Article |
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We studied whether 5-HT3 and 5HT4 receptors are involved in mediating the adaptive mechanisms following parasympathetic denervation. Methods Parasympathetic denervation of the entire colon was achieved by bilateral pelvic nerve transection and truncal vagotomy in rats. Colonic transit was measured by calculating the geometric center (GC) of51 Cr distribution. Expression of 5-HT3 and 5HT4 receptor mRNA was determined by real time RT-PCR. Results Parasympathetic denervation caused a significant delay in colonic transit (GC = 4.36) at postoperative day (POD) 1, compared with sham operation (GC = 6.31). Delayed transit was gradually restored by POD 7 (GC = 5.99) after the denervation. Restored colonic transit was antagonized by the administration of 5-HT3 and 5HT4 receptors antagonists at POD 7. 5-HT3 and 5HT4 receptors mRNA expression were significantly increased in the mucosal/submucosal layer at POD 3 or POD 7, whereas no significant difference was observed in the longitudinal muscle layers adherent with the myenteric plexus (LMMP). Conclusions It is suggested that up-regulation of 5-HT3 and 5-HT4 receptors expression in the mucosal/submucosal layer is involved to restore the delayed transit after the parasympathetic denervation in rats.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2010.05.002</identifier><identifier>PMID: 20691988</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>5-HT3 receptor ; 5-HT4 receptor ; Adaptation, Physiological - physiology ; Animals ; Biological and medical sciences ; colon ; Colon - innervation ; Colon - physiology ; denervation ; Gastrointestinal Motility - drug effects ; Gastrointestinal Motility - physiology ; General aspects ; Intestinal Mucosa - innervation ; Intestinal Mucosa - physiology ; Male ; Medical sciences ; Myenteric Plexus - physiology ; Nerve Regeneration - physiology ; Parasympathectomy ; parasympathetic ; Parasympathetic Nervous System - physiology ; Rats ; Rats, Sprague-Dawley ; Receptors, Serotonin, 5-HT3 - genetics ; Receptors, Serotonin, 5-HT3 - physiology ; Receptors, Serotonin, 5-HT4 - genetics ; Receptors, Serotonin, 5-HT4 - physiology ; RNA, Messenger - metabolism ; Serotonin 5-HT3 Receptor Antagonists - pharmacology ; Serotonin 5-HT4 Receptor Antagonists - pharmacology ; Surgery ; Up-Regulation - physiology</subject><ispartof>The Journal of surgical research, 2011-12, Vol.171 (2), p.510-516</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c601t-6c406a603788e080b04f8a25e2cab6204a730b532b2bdc9cba9dade0044de72d3</citedby><cites>FETCH-LOGICAL-c601t-6c406a603788e080b04f8a25e2cab6204a730b532b2bdc9cba9dade0044de72d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S002248041000452X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25229832$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20691988$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tong, Weidong, M.D</creatorcontrib><creatorcontrib>Kamiyama, Yoichi, M.D., Ph.D</creatorcontrib><creatorcontrib>Ridolfi, Tim J., M.D</creatorcontrib><creatorcontrib>Zietlow, Aaron, B.S</creatorcontrib><creatorcontrib>Zheng, Jun, M.D., Ph.D</creatorcontrib><creatorcontrib>Kosinski, Lauren, M.D</creatorcontrib><creatorcontrib>Ludwig, Kirk, M.D</creatorcontrib><creatorcontrib>Takahashi, Toku, M.D., Ph.D</creatorcontrib><title>The Role of 5-HT3 and 5-HT4 Receptors in the Adaptive Mechanism of Colonic Transit Following the Parasympathetic Denervation in Rats</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Background Clinical studies show that disturbed colonic motility induced by extrinsic nerves damage is restored over time. We studied whether 5-HT3 and 5HT4 receptors are involved in mediating the adaptive mechanisms following parasympathetic denervation. Methods Parasympathetic denervation of the entire colon was achieved by bilateral pelvic nerve transection and truncal vagotomy in rats. Colonic transit was measured by calculating the geometric center (GC) of51 Cr distribution. Expression of 5-HT3 and 5HT4 receptor mRNA was determined by real time RT-PCR. Results Parasympathetic denervation caused a significant delay in colonic transit (GC = 4.36) at postoperative day (POD) 1, compared with sham operation (GC = 6.31). Delayed transit was gradually restored by POD 7 (GC = 5.99) after the denervation. Restored colonic transit was antagonized by the administration of 5-HT3 and 5HT4 receptors antagonists at POD 7. 5-HT3 and 5HT4 receptors mRNA expression were significantly increased in the mucosal/submucosal layer at POD 3 or POD 7, whereas no significant difference was observed in the longitudinal muscle layers adherent with the myenteric plexus (LMMP). Conclusions It is suggested that up-regulation of 5-HT3 and 5-HT4 receptors expression in the mucosal/submucosal layer is involved to restore the delayed transit after the parasympathetic denervation in rats.</description><subject>5-HT3 receptor</subject><subject>5-HT4 receptor</subject><subject>Adaptation, Physiological - physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>colon</subject><subject>Colon - innervation</subject><subject>Colon - physiology</subject><subject>denervation</subject><subject>Gastrointestinal Motility - drug effects</subject><subject>Gastrointestinal Motility - physiology</subject><subject>General aspects</subject><subject>Intestinal Mucosa - innervation</subject><subject>Intestinal Mucosa - physiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Myenteric Plexus - physiology</subject><subject>Nerve Regeneration - physiology</subject><subject>Parasympathectomy</subject><subject>parasympathetic</subject><subject>Parasympathetic Nervous System - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Serotonin, 5-HT3 - genetics</subject><subject>Receptors, Serotonin, 5-HT3 - physiology</subject><subject>Receptors, Serotonin, 5-HT4 - genetics</subject><subject>Receptors, Serotonin, 5-HT4 - physiology</subject><subject>RNA, Messenger - metabolism</subject><subject>Serotonin 5-HT3 Receptor Antagonists - pharmacology</subject><subject>Serotonin 5-HT4 Receptor Antagonists - pharmacology</subject><subject>Surgery</subject><subject>Up-Regulation - physiology</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk1vEzEQhlcIRNPCD-CC9oI4bZj1fnmFVKkKlCIVgUKQuFmz3knj4NiL7aTKnR-Otwnl48DJHvt5X1vzTpI8y2GaQ16_Wk_X3k8ZxBqqKQB7kExyaKuM103xMJnEE5aVHMqT5NT7NcS6bYrHyQmDus1bzifJj8WK0rnVlNplWmVXiyJF09_tynROkoZgnU-VSUMEL3ocgtpR-oHkCo3ym1E2s9oaJdOFQ-NVSC-t1vZWmZs7zSd06PebAWMRIvWGDLkdBmXNaDvH4J8kj5aoPT09rmfJl8u3i9lVdv3x3fvZxXUma8hDVssSaqyhaDgn4NBBueTIKmISu5pBiU0BXVWwjnW9bGWHbY89AZRlTw3ri7Pk_OA7bLsN9ZJMcKjF4NQG3V5YVOLvG6NW4sbuRGwbZ3URDV4eDZz9viUfxEZ5SVqjIbv1ooUSoGk4RDI_kNJZ7x0t71_JQYzhibWI4YkxPAGViNFEzfM_v3ev-JVWBF4cAfQS9TL2Wyr_m6sYa3kxGr0-cBSbuVPkhJeKjKReOZJB9Fb99xvn_6ilVjFf1N9oT35tt87ElEQuPBMgPo9TNg5ZHuerrNjX4ifnE80x</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Tong, Weidong, M.D</creator><creator>Kamiyama, Yoichi, M.D., Ph.D</creator><creator>Ridolfi, Tim J., M.D</creator><creator>Zietlow, Aaron, B.S</creator><creator>Zheng, Jun, M.D., Ph.D</creator><creator>Kosinski, Lauren, M.D</creator><creator>Ludwig, Kirk, M.D</creator><creator>Takahashi, Toku, M.D., Ph.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20111201</creationdate><title>The Role of 5-HT3 and 5-HT4 Receptors in the Adaptive Mechanism of Colonic Transit Following the Parasympathetic Denervation in Rats</title><author>Tong, Weidong, M.D ; Kamiyama, Yoichi, M.D., Ph.D ; Ridolfi, Tim J., M.D ; Zietlow, Aaron, B.S ; Zheng, Jun, M.D., Ph.D ; Kosinski, Lauren, M.D ; Ludwig, Kirk, M.D ; Takahashi, Toku, M.D., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c601t-6c406a603788e080b04f8a25e2cab6204a730b532b2bdc9cba9dade0044de72d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>5-HT3 receptor</topic><topic>5-HT4 receptor</topic><topic>Adaptation, Physiological - physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>colon</topic><topic>Colon - innervation</topic><topic>Colon - physiology</topic><topic>denervation</topic><topic>Gastrointestinal Motility - drug effects</topic><topic>Gastrointestinal Motility - physiology</topic><topic>General aspects</topic><topic>Intestinal Mucosa - innervation</topic><topic>Intestinal Mucosa - physiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Myenteric Plexus - physiology</topic><topic>Nerve Regeneration - physiology</topic><topic>Parasympathectomy</topic><topic>parasympathetic</topic><topic>Parasympathetic Nervous System - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Serotonin, 5-HT3 - genetics</topic><topic>Receptors, Serotonin, 5-HT3 - physiology</topic><topic>Receptors, Serotonin, 5-HT4 - genetics</topic><topic>Receptors, Serotonin, 5-HT4 - physiology</topic><topic>RNA, Messenger - metabolism</topic><topic>Serotonin 5-HT3 Receptor Antagonists - pharmacology</topic><topic>Serotonin 5-HT4 Receptor Antagonists - pharmacology</topic><topic>Surgery</topic><topic>Up-Regulation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tong, Weidong, M.D</creatorcontrib><creatorcontrib>Kamiyama, Yoichi, M.D., Ph.D</creatorcontrib><creatorcontrib>Ridolfi, Tim J., M.D</creatorcontrib><creatorcontrib>Zietlow, Aaron, B.S</creatorcontrib><creatorcontrib>Zheng, Jun, M.D., Ph.D</creatorcontrib><creatorcontrib>Kosinski, Lauren, M.D</creatorcontrib><creatorcontrib>Ludwig, Kirk, M.D</creatorcontrib><creatorcontrib>Takahashi, Toku, M.D., Ph.D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tong, Weidong, M.D</au><au>Kamiyama, Yoichi, M.D., Ph.D</au><au>Ridolfi, Tim J., M.D</au><au>Zietlow, Aaron, B.S</au><au>Zheng, Jun, M.D., Ph.D</au><au>Kosinski, Lauren, M.D</au><au>Ludwig, Kirk, M.D</au><au>Takahashi, Toku, M.D., Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Role of 5-HT3 and 5-HT4 Receptors in the Adaptive Mechanism of Colonic Transit Following the Parasympathetic Denervation in Rats</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>171</volume><issue>2</issue><spage>510</spage><epage>516</epage><pages>510-516</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><abstract>Background Clinical studies show that disturbed colonic motility induced by extrinsic nerves damage is restored over time. We studied whether 5-HT3 and 5HT4 receptors are involved in mediating the adaptive mechanisms following parasympathetic denervation. Methods Parasympathetic denervation of the entire colon was achieved by bilateral pelvic nerve transection and truncal vagotomy in rats. Colonic transit was measured by calculating the geometric center (GC) of51 Cr distribution. Expression of 5-HT3 and 5HT4 receptor mRNA was determined by real time RT-PCR. Results Parasympathetic denervation caused a significant delay in colonic transit (GC = 4.36) at postoperative day (POD) 1, compared with sham operation (GC = 6.31). Delayed transit was gradually restored by POD 7 (GC = 5.99) after the denervation. Restored colonic transit was antagonized by the administration of 5-HT3 and 5HT4 receptors antagonists at POD 7. 5-HT3 and 5HT4 receptors mRNA expression were significantly increased in the mucosal/submucosal layer at POD 3 or POD 7, whereas no significant difference was observed in the longitudinal muscle layers adherent with the myenteric plexus (LMMP). Conclusions It is suggested that up-regulation of 5-HT3 and 5-HT4 receptors expression in the mucosal/submucosal layer is involved to restore the delayed transit after the parasympathetic denervation in rats.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>20691988</pmid><doi>10.1016/j.jss.2010.05.002</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 5-HT3 receptor 5-HT4 receptor Adaptation, Physiological - physiology Animals Biological and medical sciences colon Colon - innervation Colon - physiology denervation Gastrointestinal Motility - drug effects Gastrointestinal Motility - physiology General aspects Intestinal Mucosa - innervation Intestinal Mucosa - physiology Male Medical sciences Myenteric Plexus - physiology Nerve Regeneration - physiology Parasympathectomy parasympathetic Parasympathetic Nervous System - physiology Rats Rats, Sprague-Dawley Receptors, Serotonin, 5-HT3 - genetics Receptors, Serotonin, 5-HT3 - physiology Receptors, Serotonin, 5-HT4 - genetics Receptors, Serotonin, 5-HT4 - physiology RNA, Messenger - metabolism Serotonin 5-HT3 Receptor Antagonists - pharmacology Serotonin 5-HT4 Receptor Antagonists - pharmacology Surgery Up-Regulation - physiology |
| title | The Role of 5-HT3 and 5-HT4 Receptors in the Adaptive Mechanism of Colonic Transit Following the Parasympathetic Denervation in Rats |
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