Satellite cell number and cell cycle kinetics in response to acute myotrauma in humans: immunohistochemistry versus flow cytometry

In humans, muscle satellite cell (SC) enumeration is an important measurement used to determine the myogenic response to various stimuli. To date, the standard practice for enumeration is immunohistochemistry (IHC) using antibodies against common SC markers (Pax7, NCAM). Flow cytometry (FC) analysis...

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Veröffentlicht in:	The Journal of physiology 2010-09, Vol.588 (17), p.3307-3320
Hauptverfasser:	McKay, Bryon R., Toth, Kyle G., Tarnopolsky, Mark A., Parise, Gianni
Format:	Artikel
Sprache:	eng
Schlagworte:	Acute Disease Cell adhesion & migration Cell Count - methods Cell cycle Cell Cycle - physiology Cytokinesis - physiology Flow cytometry Flow Cytometry - methods Humans Male Muscle Contraction - physiology Quadriceps Muscle - chemistry Quadriceps Muscle - injuries Quadriceps Muscle - pathology Satellite Cells, Skeletal Muscle - chemistry Satellite Cells, Skeletal Muscle - metabolism Satellite Cells, Skeletal Muscle - pathology Satellites Skeletal Muscle and Exercise Young Adult
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