Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores
Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorop...
Gespeichert in:
| Veröffentlicht in: | Journal of the American Chemical Society 2010-11, Vol.132 (43), p.15099-15101 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 15101 |
|---|---|
| container_issue | 43 |
| container_start_page | 15099 |
| container_title | Journal of the American Chemical Society |
| container_volume | 132 |
| creator | Lee, Hsiao-lu D Lord, Samuel J Iwanaga, Shigeki Zhan, Ke Xie, Hexin Williams, Jarrod C Wang, Hui Bowman, Grant R Goley, Erin D Shapiro, Lucy Twieg, Robert J Rao, Jianghong Moerner, W. E |
| description | Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push−pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells. |
| doi_str_mv | 10.1021/ja1044192 |
| format | Article |
| fullrecord | <record><control><sourceid>acs_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2972741</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>b630463960</sourcerecordid><originalsourceid>FETCH-LOGICAL-a404t-a650fd2a98a80a396d231074968ac0eb0f6f491d5cffe50a297a77457ce9b1533</originalsourceid><addsrcrecordid>eNptkM9LwzAUx4MoOqcH_wHJxYOH6kua_shFkOF0MNjA7Vxe27TL6JKRtEP_ezumQ8FTXt775PPIl5AbBg8MOHtcIwMhmOQnZMAiDkHEeHxKBgDAgySNwwty6f26vwqesnNywUGGcQpyQNx7t1XOKW-brtXW0MkGa21qaiu6QFerVpV07myrtPFUGzrWH30HTUmnercHR6ppPF36fT1f2dZi0eodtpg3is5cjUYXdNx01tntyvabrshZhY1X19_nkCzHL4vRWzCdvU5Gz9MABYg2wDiCquQoU0wBQxmXPGSQCBmnWIDKoYorIVkZFVWlIkAuE0wSESWFkjmLwnBIng7ebZdvVFko0zpssq3TG3SfmUWd_Z0Yvcpqu8t6E08E6wX3B0HhrPdOVce3DLJ98Nkx-J69_b3sSP4k3QN3BwALn61t50z_939EXzUHjHM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Lee, Hsiao-lu D ; Lord, Samuel J ; Iwanaga, Shigeki ; Zhan, Ke ; Xie, Hexin ; Williams, Jarrod C ; Wang, Hui ; Bowman, Grant R ; Goley, Erin D ; Shapiro, Lucy ; Twieg, Robert J ; Rao, Jianghong ; Moerner, W. E</creator><creatorcontrib>Lee, Hsiao-lu D ; Lord, Samuel J ; Iwanaga, Shigeki ; Zhan, Ke ; Xie, Hexin ; Williams, Jarrod C ; Wang, Hui ; Bowman, Grant R ; Goley, Erin D ; Shapiro, Lucy ; Twieg, Robert J ; Rao, Jianghong ; Moerner, W. E</creatorcontrib><description>Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push−pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja1044192</identifier><identifier>PMID: 20936809</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Absorption ; Caulobacter crescentus - cytology ; Caulobacter crescentus - metabolism ; Cell Survival ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - metabolism ; Furans - chemistry ; Furans - metabolism ; HeLa Cells ; Humans ; Molecular Imaging - methods ; Nitriles - chemistry ; Nitriles - metabolism ; Photochemical Processes ; Proteins - metabolism</subject><ispartof>Journal of the American Chemical Society, 2010-11, Vol.132 (43), p.15099-15101</ispartof><rights>Copyright © 2010 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a404t-a650fd2a98a80a396d231074968ac0eb0f6f491d5cffe50a297a77457ce9b1533</citedby><cites>FETCH-LOGICAL-a404t-a650fd2a98a80a396d231074968ac0eb0f6f491d5cffe50a297a77457ce9b1533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja1044192$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja1044192$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20936809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Hsiao-lu D</creatorcontrib><creatorcontrib>Lord, Samuel J</creatorcontrib><creatorcontrib>Iwanaga, Shigeki</creatorcontrib><creatorcontrib>Zhan, Ke</creatorcontrib><creatorcontrib>Xie, Hexin</creatorcontrib><creatorcontrib>Williams, Jarrod C</creatorcontrib><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Bowman, Grant R</creatorcontrib><creatorcontrib>Goley, Erin D</creatorcontrib><creatorcontrib>Shapiro, Lucy</creatorcontrib><creatorcontrib>Twieg, Robert J</creatorcontrib><creatorcontrib>Rao, Jianghong</creatorcontrib><creatorcontrib>Moerner, W. E</creatorcontrib><title>Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push−pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.</description><subject>Absorption</subject><subject>Caulobacter crescentus - cytology</subject><subject>Caulobacter crescentus - metabolism</subject><subject>Cell Survival</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Furans - chemistry</subject><subject>Furans - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Molecular Imaging - methods</subject><subject>Nitriles - chemistry</subject><subject>Nitriles - metabolism</subject><subject>Photochemical Processes</subject><subject>Proteins - metabolism</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM9LwzAUx4MoOqcH_wHJxYOH6kua_shFkOF0MNjA7Vxe27TL6JKRtEP_ezumQ8FTXt775PPIl5AbBg8MOHtcIwMhmOQnZMAiDkHEeHxKBgDAgySNwwty6f26vwqesnNywUGGcQpyQNx7t1XOKW-brtXW0MkGa21qaiu6QFerVpV07myrtPFUGzrWH30HTUmnercHR6ppPF36fT1f2dZi0eodtpg3is5cjUYXdNx01tntyvabrshZhY1X19_nkCzHL4vRWzCdvU5Gz9MABYg2wDiCquQoU0wBQxmXPGSQCBmnWIDKoYorIVkZFVWlIkAuE0wSESWFkjmLwnBIng7ebZdvVFko0zpssq3TG3SfmUWd_Z0Yvcpqu8t6E08E6wX3B0HhrPdOVce3DLJ98Nkx-J69_b3sSP4k3QN3BwALn61t50z_939EXzUHjHM</recordid><startdate>20101103</startdate><enddate>20101103</enddate><creator>Lee, Hsiao-lu D</creator><creator>Lord, Samuel J</creator><creator>Iwanaga, Shigeki</creator><creator>Zhan, Ke</creator><creator>Xie, Hexin</creator><creator>Williams, Jarrod C</creator><creator>Wang, Hui</creator><creator>Bowman, Grant R</creator><creator>Goley, Erin D</creator><creator>Shapiro, Lucy</creator><creator>Twieg, Robert J</creator><creator>Rao, Jianghong</creator><creator>Moerner, W. E</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20101103</creationdate><title>Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores</title><author>Lee, Hsiao-lu D ; Lord, Samuel J ; Iwanaga, Shigeki ; Zhan, Ke ; Xie, Hexin ; Williams, Jarrod C ; Wang, Hui ; Bowman, Grant R ; Goley, Erin D ; Shapiro, Lucy ; Twieg, Robert J ; Rao, Jianghong ; Moerner, W. E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a404t-a650fd2a98a80a396d231074968ac0eb0f6f491d5cffe50a297a77457ce9b1533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Absorption</topic><topic>Caulobacter crescentus - cytology</topic><topic>Caulobacter crescentus - metabolism</topic><topic>Cell Survival</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Furans - chemistry</topic><topic>Furans - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Molecular Imaging - methods</topic><topic>Nitriles - chemistry</topic><topic>Nitriles - metabolism</topic><topic>Photochemical Processes</topic><topic>Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Hsiao-lu D</creatorcontrib><creatorcontrib>Lord, Samuel J</creatorcontrib><creatorcontrib>Iwanaga, Shigeki</creatorcontrib><creatorcontrib>Zhan, Ke</creatorcontrib><creatorcontrib>Xie, Hexin</creatorcontrib><creatorcontrib>Williams, Jarrod C</creatorcontrib><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Bowman, Grant R</creatorcontrib><creatorcontrib>Goley, Erin D</creatorcontrib><creatorcontrib>Shapiro, Lucy</creatorcontrib><creatorcontrib>Twieg, Robert J</creatorcontrib><creatorcontrib>Rao, Jianghong</creatorcontrib><creatorcontrib>Moerner, W. E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Hsiao-lu D</au><au>Lord, Samuel J</au><au>Iwanaga, Shigeki</au><au>Zhan, Ke</au><au>Xie, Hexin</au><au>Williams, Jarrod C</au><au>Wang, Hui</au><au>Bowman, Grant R</au><au>Goley, Erin D</au><au>Shapiro, Lucy</au><au>Twieg, Robert J</au><au>Rao, Jianghong</au><au>Moerner, W. E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2010-11-03</date><risdate>2010</risdate><volume>132</volume><issue>43</issue><spage>15099</spage><epage>15101</epage><pages>15099-15101</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><abstract>Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push−pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>20936809</pmid><doi>10.1021/ja1044192</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-7863 |
| ispartof | Journal of the American Chemical Society, 2010-11, Vol.132 (43), p.15099-15101 |
| issn | 0002-7863 1520-5126 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2972741 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Absorption Caulobacter crescentus - cytology Caulobacter crescentus - metabolism Cell Survival Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Furans - chemistry Furans - metabolism HeLa Cells Humans Molecular Imaging - methods Nitriles - chemistry Nitriles - metabolism Photochemical Processes Proteins - metabolism |
| title | Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T02%3A52%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Superresolution%20Imaging%20of%20Targeted%20Proteins%20in%20Fixed%20and%20Living%20Cells%20Using%20Photoactivatable%20Organic%20Fluorophores&rft.jtitle=Journal%20of%20the%20American%20Chemical%20Society&rft.au=Lee,%20Hsiao-lu%20D&rft.date=2010-11-03&rft.volume=132&rft.issue=43&rft.spage=15099&rft.epage=15101&rft.pages=15099-15101&rft.issn=0002-7863&rft.eissn=1520-5126&rft_id=info:doi/10.1021/ja1044192&rft_dat=%3Cacs_pubme%3Eb630463960%3C/acs_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/20936809&rfr_iscdi=true |