Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones
Here we report the use of a multi-genome DNA microarray to elucidate the genomic events associated with the emergence of the clonal variants of Haemophilus influenzae biogroup aegyptius causing Brazilian Purpuric Fever (BPF), an important pediatric disease with a high mortality rate. We performed di...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2010-11, Vol.96 (5), p.290-302 |
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| creator | Papazisi, Leka Ratnayake, Shashikala Remortel, Brian G. Bock, Geoffrey R. Liang, Wei Saeed, Alexander I. Liu, Jia Fleischmann, Robert D. Kilian, Mogens Peterson, Scott N. |
| description | Here we report the use of a multi-genome DNA microarray to elucidate the genomic events associated with the emergence of the clonal variants of
Haemophilus influenzae biogroup aegyptius causing Brazilian Purpuric Fever (BPF), an important pediatric disease with a high mortality rate. We performed directed genome sequencing of strain HK1212 unique loci to construct a species DNA microarray. Comparative genome hybridization using this microarray enabled us to determine and compare gene complements, and infer reliable phylogenomic relationships among members of the species. The higher genomic variability observed in the genomes of BPF-related strains (clones) and their close relatives may be characterized by significant gene flux related to a subset of functional role categories. We found that the acquisition of a large number of virulence determinants featuring numerous cell membrane proteins coupled to the loss of genes involved in transport, central biosynthetic pathways and in particular, energy production pathways to be characteristics of the BPF genomic variants. |
| doi_str_mv | 10.1016/j.ygeno.2010.07.005 |
| format | Article |
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Haemophilus influenzae biogroup aegyptius causing Brazilian Purpuric Fever (BPF), an important pediatric disease with a high mortality rate. We performed directed genome sequencing of strain HK1212 unique loci to construct a species DNA microarray. Comparative genome hybridization using this microarray enabled us to determine and compare gene complements, and infer reliable phylogenomic relationships among members of the species. The higher genomic variability observed in the genomes of BPF-related strains (clones) and their close relatives may be characterized by significant gene flux related to a subset of functional role categories. We found that the acquisition of a large number of virulence determinants featuring numerous cell membrane proteins coupled to the loss of genes involved in transport, central biosynthetic pathways and in particular, energy production pathways to be characteristics of the BPF genomic variants.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2010.07.005</identifier><identifier>PMID: 20654709</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Bacterial Proteins - genetics ; Biological and medical sciences ; Brazil ; Brazilian Purpuric Fever ; Cell membranes ; Comparative Genomic Hybridization ; Comparative genomics ; Fever - microbiology ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Genetic Variation ; Genetics of eukaryotes. Biological and molecular evolution ; Genome, Bacterial ; Haemophilus ; Haemophilus Infections - microbiology ; Haemophilus influenzae ; Haemophilus influenzae - classification ; Haemophilus influenzae - genetics ; Haemophilus influenzae - pathogenicity ; Humans ; Microarray ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Oligonucleotide Array Sequence Analysis ; Pathogen emergence ; Phylogeny ; Purpura - microbiology ; Sequence Analysis, DNA ; Virulence ; Virulence Factors - genetics</subject><ispartof>Genomics (San Diego, Calif.), 2010-11, Vol.96 (5), p.290-302</ispartof><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><rights>2010 Elsevier Inc. All rights reserved. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-b5d42c857567f3781e3b89fc217960b3195a79f26364dbf30c4e68ce8eb9e94d3</citedby><cites>FETCH-LOGICAL-c520t-b5d42c857567f3781e3b89fc217960b3195a79f26364dbf30c4e68ce8eb9e94d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754310001588$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23448727$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20654709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Papazisi, Leka</creatorcontrib><creatorcontrib>Ratnayake, Shashikala</creatorcontrib><creatorcontrib>Remortel, Brian G.</creatorcontrib><creatorcontrib>Bock, Geoffrey R.</creatorcontrib><creatorcontrib>Liang, Wei</creatorcontrib><creatorcontrib>Saeed, Alexander I.</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Fleischmann, Robert D.</creatorcontrib><creatorcontrib>Kilian, Mogens</creatorcontrib><creatorcontrib>Peterson, Scott N.</creatorcontrib><title>Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Here we report the use of a multi-genome DNA microarray to elucidate the genomic events associated with the emergence of the clonal variants of
Haemophilus influenzae biogroup aegyptius causing Brazilian Purpuric Fever (BPF), an important pediatric disease with a high mortality rate. We performed directed genome sequencing of strain HK1212 unique loci to construct a species DNA microarray. Comparative genome hybridization using this microarray enabled us to determine and compare gene complements, and infer reliable phylogenomic relationships among members of the species. The higher genomic variability observed in the genomes of BPF-related strains (clones) and their close relatives may be characterized by significant gene flux related to a subset of functional role categories. We found that the acquisition of a large number of virulence determinants featuring numerous cell membrane proteins coupled to the loss of genes involved in transport, central biosynthetic pathways and in particular, energy production pathways to be characteristics of the BPF genomic variants.</description><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Brazil</subject><subject>Brazilian Purpuric Fever</subject><subject>Cell membranes</subject><subject>Comparative Genomic Hybridization</subject><subject>Comparative genomics</subject><subject>Fever - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Genetic Variation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genome, Bacterial</subject><subject>Haemophilus</subject><subject>Haemophilus Infections - microbiology</subject><subject>Haemophilus influenzae</subject><subject>Haemophilus influenzae - classification</subject><subject>Haemophilus influenzae - genetics</subject><subject>Haemophilus influenzae - pathogenicity</subject><subject>Humans</subject><subject>Microarray</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pathogen emergence</subject><subject>Phylogeny</subject><subject>Purpura - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Virulence</subject><subject>Virulence Factors - genetics</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-O0zAQhyMEYsvCEyAhXxBwSLHjvzmAxK4oi7QSe1jOluNMWldOXOykUvcpeGQcWha4wMmS55ufZuYriucELwkm4u12eVjDEJYVzj9YLjHmD4oFwaoulWDiYbHASqlSckbPiicpbTHGNVXV4-KswoIzietF8f02GuuGNdptDj7Meb2zCPYwjAl5MO1cGwNq3R5icuMBhQ5dGejDbuP8lJAbOj_BcGcAmaFF4wYQ9BBzkoWZvYjmznlnBnQzxd0Uc_oqx0f0-uJm9aY0KQXrzAgtsj4MkJ4WjzrjEzw7vefF19XH28ur8vrLp8-XH65Lyys8lg1vWWUVl1zIjkpFgDaq7mxFZC1wQ0nNjay7SlDB2qaj2DIQyoKCpoaatfS8eH_M3U1ND63NC0fj9S663sSDDsbpvyuD2-h12OuqFhJTlgNenQJi-DZBGnXvkgXvzQBhSloRzhkhHP-XlAJXlGZZmaRH0saQUoTufh6C9Sxdb_VP6XqWrrHUWXruevHnKvc9vyxn4OUJMMka30UzWJd-c5QxJSuZuXdHDvLh9w6iTtbNIlsXwY66De6fg_wAb3rPBw</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Papazisi, Leka</creator><creator>Ratnayake, Shashikala</creator><creator>Remortel, Brian G.</creator><creator>Bock, Geoffrey R.</creator><creator>Liang, Wei</creator><creator>Saeed, Alexander I.</creator><creator>Liu, Jia</creator><creator>Fleischmann, Robert D.</creator><creator>Kilian, Mogens</creator><creator>Peterson, Scott N.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20101101</creationdate><title>Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones</title><author>Papazisi, Leka ; Ratnayake, Shashikala ; Remortel, Brian G. ; Bock, Geoffrey R. ; Liang, Wei ; Saeed, Alexander I. ; Liu, Jia ; Fleischmann, Robert D. ; Kilian, Mogens ; Peterson, Scott N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-b5d42c857567f3781e3b89fc217960b3195a79f26364dbf30c4e68ce8eb9e94d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Brazil</topic><topic>Brazilian Purpuric Fever</topic><topic>Cell membranes</topic><topic>Comparative Genomic Hybridization</topic><topic>Comparative genomics</topic><topic>Fever - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Genetic Variation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genome, Bacterial</topic><topic>Haemophilus</topic><topic>Haemophilus Infections - microbiology</topic><topic>Haemophilus influenzae</topic><topic>Haemophilus influenzae - classification</topic><topic>Haemophilus influenzae - genetics</topic><topic>Haemophilus influenzae - pathogenicity</topic><topic>Humans</topic><topic>Microarray</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pathogen emergence</topic><topic>Phylogeny</topic><topic>Purpura - microbiology</topic><topic>Sequence Analysis, DNA</topic><topic>Virulence</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Papazisi, Leka</creatorcontrib><creatorcontrib>Ratnayake, Shashikala</creatorcontrib><creatorcontrib>Remortel, Brian G.</creatorcontrib><creatorcontrib>Bock, Geoffrey R.</creatorcontrib><creatorcontrib>Liang, Wei</creatorcontrib><creatorcontrib>Saeed, Alexander I.</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Fleischmann, Robert D.</creatorcontrib><creatorcontrib>Kilian, Mogens</creatorcontrib><creatorcontrib>Peterson, Scott N.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Papazisi, Leka</au><au>Ratnayake, Shashikala</au><au>Remortel, Brian G.</au><au>Bock, Geoffrey R.</au><au>Liang, Wei</au><au>Saeed, Alexander I.</au><au>Liu, Jia</au><au>Fleischmann, Robert D.</au><au>Kilian, Mogens</au><au>Peterson, Scott N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>96</volume><issue>5</issue><spage>290</spage><epage>302</epage><pages>290-302</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Here we report the use of a multi-genome DNA microarray to elucidate the genomic events associated with the emergence of the clonal variants of
Haemophilus influenzae biogroup aegyptius causing Brazilian Purpuric Fever (BPF), an important pediatric disease with a high mortality rate. We performed directed genome sequencing of strain HK1212 unique loci to construct a species DNA microarray. Comparative genome hybridization using this microarray enabled us to determine and compare gene complements, and infer reliable phylogenomic relationships among members of the species. The higher genomic variability observed in the genomes of BPF-related strains (clones) and their close relatives may be characterized by significant gene flux related to a subset of functional role categories. We found that the acquisition of a large number of virulence determinants featuring numerous cell membrane proteins coupled to the loss of genes involved in transport, central biosynthetic pathways and in particular, energy production pathways to be characteristics of the BPF genomic variants.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20654709</pmid><doi>10.1016/j.ygeno.2010.07.005</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial Proteins - genetics Biological and medical sciences Brazil Brazilian Purpuric Fever Cell membranes Comparative Genomic Hybridization Comparative genomics Fever - microbiology Fundamental and applied biological sciences. Psychology Genes. Genome Genetic Variation Genetics of eukaryotes. Biological and molecular evolution Genome, Bacterial Haemophilus Haemophilus Infections - microbiology Haemophilus influenzae Haemophilus influenzae - classification Haemophilus influenzae - genetics Haemophilus influenzae - pathogenicity Humans Microarray Molecular and cellular biology Molecular genetics Molecular Sequence Data Oligonucleotide Array Sequence Analysis Pathogen emergence Phylogeny Purpura - microbiology Sequence Analysis, DNA Virulence Virulence Factors - genetics |
| title | Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones |
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