Inhibitory Effect of Chinese Propolis on Phosphatidylcholine-Specific Phospholipase C Activity in Vascular Endothelial Cells
To understand the mechanisms underlying the anti-inflammatory action of Chinese propolis, we investigated its effect on the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) that plays critical roles in control of vascular endothelial cell (VEC) function and inflammatory responses. F...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2011-01, Vol.2011 (2011), p.1-8 |
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| description | To understand the mechanisms underlying the anti-inflammatory action of Chinese propolis, we investigated its effect on the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) that plays critical roles in control of vascular endothelial cell (VEC) function and inflammatory responses. Furthermore, p53 and reactive oxygen species (ROS) levels and mitochondrial membrane potential (Δψm) were investigated. Our data indicated that treatment of Chinese propolis 6.25 and 12.5 μg/ml for 12 hours increased VEC viability obviously. Exposure to Chinese propolis 6.25, 12.5, and 25 μg/ml for 6 and 12 hours significantly decreased PC-PLC activity and p53 level, and ROS levels were depressed by Chinese propolis 12.5 μg/ml and 25 μg/ml dramatically. The Δψm of VECs was not affected by Chinese propolis at low concentration but disrupted by the propolis at 25 μg/ml significantly, which indicated that Chinese propolis depressed PC-PLC activity and the levels of p53 and ROS in VECs but disrupted Δψm at a high concentration. |
| doi_str_mv | 10.1155/2011/985278 |
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| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2963135</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2062799770</sourcerecordid><originalsourceid>FETCH-LOGICAL-c493t-88a63343be68e2d3cef9cc9ccf5efd1848e9faf3cca208a2cb3397cd5b1bc9fe3</originalsourceid><addsrcrecordid>eNqFkd9r1TAUx4sobk6ffFYCPghKXX60TfIijHKng4EDf-BbSNMTm9Gb1KSdXNgfb0avF_VBIZBwzidfzuFTFE8JfkNIXZ9STMipFDXl4l5xTHhFyooKcf_w5l-PikcpXWNMJef8YXFEsRSEyvq4uL3wg-vcHOIObawFM6NgUTs4DwnQVQxTGF1CwaOrIaRp0LPrd6MZctVD-XEC46wz-2YuTjp_a9GZmd2Nm3fIefRFJ7OMOqKN78M8wOj0iFoYx_S4eGD1mODJ_j4pPp9vPrXvy8sP7y7as8vSVJLNpRC6YaxiHTQCaM8MWGlMPrYG2xNRCZBWW2aMplhoajrGJDd93ZHOSAvspHi75k5Lt4XegJ-jHtUU3VbHnQraqT873g3qW7hRVDaMsDoHvNwHxPB9gTSrrUsmr6A9hCUp3tCqEaLimXzxF3kdlujzdorihnKZDeB_UYQTzJmsGM3U65UyMaQUwR4mJljdqVd36tWqPtPPf1_ywP5ynYFXK5Dl9vqH-0_asxWGjIDVB7gmrMlhPwG1zMLu</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1710739432</pqid></control><display><type>article</type><title>Inhibitory Effect of Chinese Propolis on Phosphatidylcholine-Specific Phospholipase C Activity in Vascular Endothelial Cells</title><source>Wiley-Blackwell Open Access Titles</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Xuan, Hongzhuan ; Zhu, Ruiliang ; Li, Yajing ; Hu, Fuliang</creator><creatorcontrib>Xuan, Hongzhuan ; Zhu, Ruiliang ; Li, Yajing ; Hu, Fuliang</creatorcontrib><description>To understand the mechanisms underlying the anti-inflammatory action of Chinese propolis, we investigated its effect on the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) that plays critical roles in control of vascular endothelial cell (VEC) function and inflammatory responses. Furthermore, p53 and reactive oxygen species (ROS) levels and mitochondrial membrane potential (Δψm) were investigated. Our data indicated that treatment of Chinese propolis 6.25 and 12.5 μg/ml for 12 hours increased VEC viability obviously. Exposure to Chinese propolis 6.25, 12.5, and 25 μg/ml for 6 and 12 hours significantly decreased PC-PLC activity and p53 level, and ROS levels were depressed by Chinese propolis 12.5 μg/ml and 25 μg/ml dramatically. The Δψm of VECs was not affected by Chinese propolis at low concentration but disrupted by the propolis at 25 μg/ml significantly, which indicated that Chinese propolis depressed PC-PLC activity and the levels of p53 and ROS in VECs but disrupted Δψm at a high concentration.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2011/985278</identifier><identifier>PMID: 20981295</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Apoptosis ; Atherosclerosis ; Biology ; Cancer therapies ; Cell adhesion & migration ; Cell culture ; Endothelial cells ; Enzymes ; Ethanol ; Gene expression ; Inflammation ; Lecithin ; Membrane potential ; Mitochondria ; p53 Protein ; Phosphatidylcholine ; Phospholipase ; Phospholipase C ; Propolis ; Proteins ; Reactive oxygen species ; Senescence ; Viability</subject><ispartof>Evidence-based complementary and alternative medicine, 2011-01, Vol.2011 (2011), p.1-8</ispartof><rights>Copyright © 2011 Hongzhuan Xuan et al.</rights><rights>Copyright © 2011 Hongzhuan Xuan et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2011 Hongzhuan Xuan et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-88a63343be68e2d3cef9cc9ccf5efd1848e9faf3cca208a2cb3397cd5b1bc9fe3</citedby><cites>FETCH-LOGICAL-c493t-88a63343be68e2d3cef9cc9ccf5efd1848e9faf3cca208a2cb3397cd5b1bc9fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2963135/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2963135/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20981295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xuan, Hongzhuan</creatorcontrib><creatorcontrib>Zhu, Ruiliang</creatorcontrib><creatorcontrib>Li, Yajing</creatorcontrib><creatorcontrib>Hu, Fuliang</creatorcontrib><title>Inhibitory Effect of Chinese Propolis on Phosphatidylcholine-Specific Phospholipase C Activity in Vascular Endothelial Cells</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>To understand the mechanisms underlying the anti-inflammatory action of Chinese propolis, we investigated its effect on the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) that plays critical roles in control of vascular endothelial cell (VEC) function and inflammatory responses. Furthermore, p53 and reactive oxygen species (ROS) levels and mitochondrial membrane potential (Δψm) were investigated. Our data indicated that treatment of Chinese propolis 6.25 and 12.5 μg/ml for 12 hours increased VEC viability obviously. Exposure to Chinese propolis 6.25, 12.5, and 25 μg/ml for 6 and 12 hours significantly decreased PC-PLC activity and p53 level, and ROS levels were depressed by Chinese propolis 12.5 μg/ml and 25 μg/ml dramatically. The Δψm of VECs was not affected by Chinese propolis at low concentration but disrupted by the propolis at 25 μg/ml significantly, which indicated that Chinese propolis depressed PC-PLC activity and the levels of p53 and ROS in VECs but disrupted Δψm at a high concentration.</description><subject>Apoptosis</subject><subject>Atherosclerosis</subject><subject>Biology</subject><subject>Cancer therapies</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Endothelial cells</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>Gene expression</subject><subject>Inflammation</subject><subject>Lecithin</subject><subject>Membrane potential</subject><subject>Mitochondria</subject><subject>p53 Protein</subject><subject>Phosphatidylcholine</subject><subject>Phospholipase</subject><subject>Phospholipase C</subject><subject>Propolis</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Senescence</subject><subject>Viability</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkd9r1TAUx4sobk6ffFYCPghKXX60TfIijHKng4EDf-BbSNMTm9Gb1KSdXNgfb0avF_VBIZBwzidfzuFTFE8JfkNIXZ9STMipFDXl4l5xTHhFyooKcf_w5l-PikcpXWNMJef8YXFEsRSEyvq4uL3wg-vcHOIObawFM6NgUTs4DwnQVQxTGF1CwaOrIaRp0LPrd6MZctVD-XEC46wz-2YuTjp_a9GZmd2Nm3fIefRFJ7OMOqKN78M8wOj0iFoYx_S4eGD1mODJ_j4pPp9vPrXvy8sP7y7as8vSVJLNpRC6YaxiHTQCaM8MWGlMPrYG2xNRCZBWW2aMplhoajrGJDd93ZHOSAvspHi75k5Lt4XegJ-jHtUU3VbHnQraqT873g3qW7hRVDaMsDoHvNwHxPB9gTSrrUsmr6A9hCUp3tCqEaLimXzxF3kdlujzdorihnKZDeB_UYQTzJmsGM3U65UyMaQUwR4mJljdqVd36tWqPtPPf1_ywP5ynYFXK5Dl9vqH-0_asxWGjIDVB7gmrMlhPwG1zMLu</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Xuan, Hongzhuan</creator><creator>Zhu, Ruiliang</creator><creator>Li, Yajing</creator><creator>Hu, Fuliang</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Inhibitory Effect of Chinese Propolis on Phosphatidylcholine-Specific Phospholipase C Activity in Vascular Endothelial Cells</title><author>Xuan, Hongzhuan ; Zhu, Ruiliang ; Li, Yajing ; Hu, Fuliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-88a63343be68e2d3cef9cc9ccf5efd1848e9faf3cca208a2cb3397cd5b1bc9fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Apoptosis</topic><topic>Atherosclerosis</topic><topic>Biology</topic><topic>Cancer therapies</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Endothelial cells</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>Gene expression</topic><topic>Inflammation</topic><topic>Lecithin</topic><topic>Membrane potential</topic><topic>Mitochondria</topic><topic>p53 Protein</topic><topic>Phosphatidylcholine</topic><topic>Phospholipase</topic><topic>Phospholipase C</topic><topic>Propolis</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Senescence</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xuan, Hongzhuan</creatorcontrib><creatorcontrib>Zhu, Ruiliang</creatorcontrib><creatorcontrib>Li, Yajing</creatorcontrib><creatorcontrib>Hu, Fuliang</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Psychology Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xuan, Hongzhuan</au><au>Zhu, Ruiliang</au><au>Li, Yajing</au><au>Hu, Fuliang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effect of Chinese Propolis on Phosphatidylcholine-Specific Phospholipase C Activity in Vascular Endothelial Cells</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>2011</volume><issue>2011</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>To understand the mechanisms underlying the anti-inflammatory action of Chinese propolis, we investigated its effect on the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) that plays critical roles in control of vascular endothelial cell (VEC) function and inflammatory responses. 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| subjects | Apoptosis Atherosclerosis Biology Cancer therapies Cell adhesion & migration Cell culture Endothelial cells Enzymes Ethanol Gene expression Inflammation Lecithin Membrane potential Mitochondria p53 Protein Phosphatidylcholine Phospholipase Phospholipase C Propolis Proteins Reactive oxygen species Senescence Viability |
| title | Inhibitory Effect of Chinese Propolis on Phosphatidylcholine-Specific Phospholipase C Activity in Vascular Endothelial Cells |
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