Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis
The selection of T cell clones with mutations in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene has been used to isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy in...
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| Veröffentlicht in: | The Journal of clinical investigation 1994-07, Vol.94 (1), p.105-109 |
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| container_title | The Journal of clinical investigation |
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| creator | Allegretta, M Albertini, R J Howell, M D Smith, L R Martin, R McFarland, H F Sriram, S Brostoff, S Steinman, L |
| description | The selection of T cell clones with mutations in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene has been used to isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining regions (CDR3) of the T cell receptor (TCR) alpha and beta chains are the putative contact sites for peptide fragments of MBP bound in the groove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP-reactive hprt-T cell clones are homologous to TCRs from other T cells relevant to MS, including T cells causing experimental allergic encephalomyelitis (EAE) and T cells found in brain lesions and in the cerebrospinal fluid (CSF) of MS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS. |
| doi_str_mv | 10.1172/JCI117295 |
| format | Article |
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These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining regions (CDR3) of the T cell receptor (TCR) alpha and beta chains are the putative contact sites for peptide fragments of MBP bound in the groove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP-reactive hprt-T cell clones are homologous to TCRs from other T cells relevant to MS, including T cells causing experimental allergic encephalomyelitis (EAE) and T cells found in brain lesions and in the cerebrospinal fluid (CSF) of MS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS.</description><identifier>ISSN: 0021-9738</identifier><identifier>DOI: 10.1172/JCI117295</identifier><identifier>PMID: 8040252</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Base Sequence ; Encephalomyelitis, Autoimmune, Experimental - immunology ; Humans ; Hypoxanthine Phosphoribosyltransferase - genetics ; Molecular Sequence Data ; Multiple Sclerosis - etiology ; Multiple Sclerosis - immunology ; Polymerase Chain Reaction ; Receptors, Antigen, T-Cell, alpha-beta - chemistry ; Receptors, Antigen, T-Cell, alpha-beta - genetics ; Sequence Homology, Amino Acid ; T-Lymphocytes - immunology</subject><ispartof>The Journal of clinical investigation, 1994-07, Vol.94 (1), p.105-109</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-fcab0d85588d54ec4fe7f64edb8ea346297bf2cacc2bbc7c993377cd95d8db6c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC296287/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC296287/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8040252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Allegretta, M</creatorcontrib><creatorcontrib>Albertini, R J</creatorcontrib><creatorcontrib>Howell, M D</creatorcontrib><creatorcontrib>Smith, L R</creatorcontrib><creatorcontrib>Martin, R</creatorcontrib><creatorcontrib>McFarland, H F</creatorcontrib><creatorcontrib>Sriram, S</creatorcontrib><creatorcontrib>Brostoff, S</creatorcontrib><creatorcontrib>Steinman, L</creatorcontrib><title>Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>The selection of T cell clones with mutations in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene has been used to isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining regions (CDR3) of the T cell receptor (TCR) alpha and beta chains are the putative contact sites for peptide fragments of MBP bound in the groove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP-reactive hprt-T cell clones are homologous to TCRs from other T cells relevant to MS, including T cells causing experimental allergic encephalomyelitis (EAE) and T cells found in brain lesions and in the cerebrospinal fluid (CSF) of MS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Encephalomyelitis, Autoimmune, Experimental - immunology</subject><subject>Humans</subject><subject>Hypoxanthine Phosphoribosyltransferase - genetics</subject><subject>Molecular Sequence Data</subject><subject>Multiple Sclerosis - etiology</subject><subject>Multiple Sclerosis - immunology</subject><subject>Polymerase Chain Reaction</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - chemistry</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>T-Lymphocytes - immunology</subject><issn>0021-9738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkbtOxDAQRV2AeBd8AJIrJIoFx3HipKBAK2BBSDRQW85kshg5dogdHr_BF-MVqxVUY2nO3LmeS8hxxs6zTPKL-_ndqtbFFtljjGezWubVLtkP4ZWxTIhC7JCdignGC75Hvhe-99YvDQbaYPxAdPSJAlpLRwQcoh_p6-QgGu-0pQHfJnSQ4MmZ9KTR036y0QwWaQCLow8mUO3atUqgPbZGR-OWFD8HHE2PLiYlbRO8NEBXcsOLtr7_QmuiCYdku9M24NG6HpDnm-un-WL28Hh7N796mEFe1nHWgW5YWxVFVbWFQBAdyq4U2DYV6lyUvJZNx0ED8KYBCXWd51JCWxdt1TYl5Afk8ld3mJpkEpKvUVs1JIt6_FJeG_W_48yLWvp3xeuSVzLNn67nR59OEaLqTVj9WTv0U1CyLLngQiTw7BeEdJ0wYrfZkTG1ykptMkvsyV9TG3IdWP4DQ7Waxg</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Allegretta, M</creator><creator>Albertini, R J</creator><creator>Howell, M D</creator><creator>Smith, L R</creator><creator>Martin, R</creator><creator>McFarland, H F</creator><creator>Sriram, S</creator><creator>Brostoff, S</creator><creator>Steinman, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19940701</creationdate><title>Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis</title><author>Allegretta, M ; Albertini, R J ; Howell, M D ; Smith, L R ; Martin, R ; McFarland, H F ; Sriram, S ; Brostoff, S ; Steinman, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-fcab0d85588d54ec4fe7f64edb8ea346297bf2cacc2bbc7c993377cd95d8db6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Encephalomyelitis, Autoimmune, Experimental - immunology</topic><topic>Humans</topic><topic>Hypoxanthine Phosphoribosyltransferase - genetics</topic><topic>Molecular Sequence Data</topic><topic>Multiple Sclerosis - etiology</topic><topic>Multiple Sclerosis - immunology</topic><topic>Polymerase Chain Reaction</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - chemistry</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allegretta, M</creatorcontrib><creatorcontrib>Albertini, R J</creatorcontrib><creatorcontrib>Howell, M D</creatorcontrib><creatorcontrib>Smith, L R</creatorcontrib><creatorcontrib>Martin, R</creatorcontrib><creatorcontrib>McFarland, H F</creatorcontrib><creatorcontrib>Sriram, S</creatorcontrib><creatorcontrib>Brostoff, S</creatorcontrib><creatorcontrib>Steinman, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Allegretta, M</au><au>Albertini, R J</au><au>Howell, M D</au><au>Smith, L R</au><au>Martin, R</au><au>McFarland, H F</au><au>Sriram, S</au><au>Brostoff, S</au><au>Steinman, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis</atitle><jtitle>The Journal of clinical investigation</jtitle><addtitle>J Clin Invest</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>94</volume><issue>1</issue><spage>105</spage><epage>109</epage><pages>105-109</pages><issn>0021-9738</issn><abstract>The selection of T cell clones with mutations in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene has been used to isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining regions (CDR3) of the T cell receptor (TCR) alpha and beta chains are the putative contact sites for peptide fragments of MBP bound in the groove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP-reactive hprt-T cell clones are homologous to TCRs from other T cells relevant to MS, including T cells causing experimental allergic encephalomyelitis (EAE) and T cells found in brain lesions and in the cerebrospinal fluid (CSF) of MS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS.</abstract><cop>United States</cop><pmid>8040252</pmid><doi>10.1172/JCI117295</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of clinical investigation, 1994-07, Vol.94 (1), p.105-109 |
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| subjects | Amino Acid Sequence Base Sequence Encephalomyelitis, Autoimmune, Experimental - immunology Humans Hypoxanthine Phosphoribosyltransferase - genetics Molecular Sequence Data Multiple Sclerosis - etiology Multiple Sclerosis - immunology Polymerase Chain Reaction Receptors, Antigen, T-Cell, alpha-beta - chemistry Receptors, Antigen, T-Cell, alpha-beta - genetics Sequence Homology, Amino Acid T-Lymphocytes - immunology |
| title | Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis |
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