Mechanism of Cellular Uptake of Highly Fluorescent Conjugated Polymer Nanoparticles
Conjugated polymer nanoparticles are formed by precipitation of highly fluorescent conjugated polymers to form small nanoparticles with extremely bright fluorescence. We characterized cellular uptake and cytotoxicity of 18 ± 5 nm PFBT conjugated polymer nanoparticles in J774A.1 cells. Significant na...
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| Veröffentlicht in: | Biomacromolecules 2010-10, Vol.11 (10), p.2675-2682 |
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| creator | Fernando, Lawrence P Kandel, Prakash K Yu, Jiangbo McNeill, Jason Ackroyd, P. Christine Christensen, Kenneth A |
| description | Conjugated polymer nanoparticles are formed by precipitation of highly fluorescent conjugated polymers to form small nanoparticles with extremely bright fluorescence. We characterized cellular uptake and cytotoxicity of 18 ± 5 nm PFBT conjugated polymer nanoparticles in J774A.1 cells. Significant nanoparticle uptake was observed, indicating efficient nanoparticle entry into cells, even for short (1 h) incubations. The high fluorescence of these nanoparticles allows extremely low loading concentrations; PFBT nanoparticle fluorescence in cells could be detected with loading concentrations of 155 pM (270 ppb). Cellular uptake slows at low temperature, consistent with endocytic entry. Nanoparticles colocalize with Texas Red dextran and are trafficked to lysosomes, as demonstrated by the location of nanoparticle fluorescence in perinuclear organelles that also stain with an anti-LAMP-1 antibody. Inhibition of uptake by phosphoinositide 3-kinase inhibitors implicates macropinocytosis as the operative endocytic mechanism. No significant cytotoxic or inflammatory effects could be observed, making PFBT nanoparticles attractive probes for live cell imaging. |
| doi_str_mv | 10.1021/bm1007103 |
| format | Article |
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Nanoparticles colocalize with Texas Red dextran and are trafficked to lysosomes, as demonstrated by the location of nanoparticle fluorescence in perinuclear organelles that also stain with an anti-LAMP-1 antibody. Inhibition of uptake by phosphoinositide 3-kinase inhibitors implicates macropinocytosis as the operative endocytic mechanism. 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Christine</creatorcontrib><creatorcontrib>Christensen, Kenneth A</creatorcontrib><title>Mechanism of Cellular Uptake of Highly Fluorescent Conjugated Polymer Nanoparticles</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>Conjugated polymer nanoparticles are formed by precipitation of highly fluorescent conjugated polymers to form small nanoparticles with extremely bright fluorescence. We characterized cellular uptake and cytotoxicity of 18 ± 5 nm PFBT conjugated polymer nanoparticles in J774A.1 cells. Significant nanoparticle uptake was observed, indicating efficient nanoparticle entry into cells, even for short (1 h) incubations. The high fluorescence of these nanoparticles allows extremely low loading concentrations; PFBT nanoparticle fluorescence in cells could be detected with loading concentrations of 155 pM (270 ppb). Cellular uptake slows at low temperature, consistent with endocytic entry. Nanoparticles colocalize with Texas Red dextran and are trafficked to lysosomes, as demonstrated by the location of nanoparticle fluorescence in perinuclear organelles that also stain with an anti-LAMP-1 antibody. Inhibition of uptake by phosphoinositide 3-kinase inhibitors implicates macropinocytosis as the operative endocytic mechanism. No significant cytotoxic or inflammatory effects could be observed, making PFBT nanoparticles attractive probes for live cell imaging.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Cell Culture Techniques</subject><subject>Cell Line</subject><subject>Exact sciences and technology</subject><subject>Flow Cytometry</subject><subject>Fluorenes - chemistry</subject><subject>Fluorenes - metabolism</subject><subject>Fluorescence</subject><subject>Forms of application and semi-finished materials</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Fluorescence</subject><subject>Miscellaneous</subject><subject>Molecular Imaging - methods</subject><subject>Nanoparticles - chemistry</subject><subject>Polymer industry, paints, wood</subject><subject>Polymers - chemistry</subject><subject>Polymers - metabolism</subject><subject>Technology of polymers</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1Lw0AQhhdRtFYP_gHJxYOH6OxHvi6CFKtC_QD1HCab3TZ1ky27idB_b2prVfA0w8wz7zu8hJxQuKDA6GVRU4CEAt8hAxqxOBQxsN2vPgqTJEsOyKH3cwDIuIj2yQGDNOaUswF5eVByhk3l68DqYKSM6Qy64G3R4rtaje6q6cwsg7HprFNeqqYNRraZd1NsVRk8W7OslQsesbELdG0ljfJHZE-j8ep4U4fkbXzzOroLJ0-396PrSYiCizbUpcYiFUyngCphjIm-auAJL0Ajy6CMoohSFAoZjfuTkhdxWsgIuMQUgQ_J1Vp30RW1Kle_OTT5wlU1umVuscr_bppqlk_tR86ymEVc9ALnawHprPdO6e0thXyVbL5NtmdPf5ttye8oe-BsA6CXaLTDRlb-h-MCEsjoD4fS53PbuabP6B_DT5UQjYc</recordid><startdate>20101011</startdate><enddate>20101011</enddate><creator>Fernando, Lawrence P</creator><creator>Kandel, Prakash K</creator><creator>Yu, Jiangbo</creator><creator>McNeill, Jason</creator><creator>Ackroyd, P. 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Christine ; Christensen, Kenneth A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a434t-fdfab842f80ae722240aef0373b0fa290d55511a4ea216434d3b68bc503ca8a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Cell Culture Techniques</topic><topic>Cell Line</topic><topic>Exact sciences and technology</topic><topic>Flow Cytometry</topic><topic>Fluorenes - chemistry</topic><topic>Fluorenes - metabolism</topic><topic>Fluorescence</topic><topic>Forms of application and semi-finished materials</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Microscopy, Atomic Force</topic><topic>Microscopy, Fluorescence</topic><topic>Miscellaneous</topic><topic>Molecular Imaging - methods</topic><topic>Nanoparticles - chemistry</topic><topic>Polymer industry, paints, wood</topic><topic>Polymers - chemistry</topic><topic>Polymers - metabolism</topic><topic>Technology of polymers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernando, Lawrence P</creatorcontrib><creatorcontrib>Kandel, Prakash K</creatorcontrib><creatorcontrib>Yu, Jiangbo</creatorcontrib><creatorcontrib>McNeill, Jason</creatorcontrib><creatorcontrib>Ackroyd, P. 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| source | MEDLINE; ACS Publications |
| subjects | Animals Applied sciences Cell Culture Techniques Cell Line Exact sciences and technology Flow Cytometry Fluorenes - chemistry Fluorenes - metabolism Fluorescence Forms of application and semi-finished materials Macrophages - metabolism Mice Microscopy, Atomic Force Microscopy, Fluorescence Miscellaneous Molecular Imaging - methods Nanoparticles - chemistry Polymer industry, paints, wood Polymers - chemistry Polymers - metabolism Technology of polymers |
| title | Mechanism of Cellular Uptake of Highly Fluorescent Conjugated Polymer Nanoparticles |
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