Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse
Mutations in FAM20C were recently identified as the cause of lethal osteosclerotic bone dysplasia, which highlighted the important role of this molecule in biomineralization. No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developme...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 2010-11, Vol.58 (11), p.957-967 |
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| creator | Wang, Xiaofang Hao, Jianjun Xie, Yixia Sun, Yao Hernandez, Brianda Yamoah, Albert K. Prasad, Monica Zhu, Qinglin Feng, Jian Q. Qin, Chunlin |
| description | Mutations in FAM20C were recently identified as the cause of lethal osteosclerotic bone dysplasia, which highlighted the important role of this molecule in biomineralization. No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developmental processes of bone and tooth. In the present study, we analyzed in detail the expression profile of FAM20C during osteogenesis and odontogenesis using ISH and IHC approaches. The specimens analyzed were mouse tissues spanning embryonic day 13.5 (E13.5) to postnatal 8 weeks. The earliest presence of FAM20C was observed at E14.5. During osteogenesis, FAM20C mRNA was detected in the chondrocytes and osteoblasts of the long bone, whereas its protein was observed in the extracellular matrix (ECM) of bone and in the cytoplasm of the chondrocytes, osteoblasts, and osteocytes. During odontogenesis, FAM20C mRNA was detected in the ameloblasts, odontoblasts, cementoblasts, and periodontal ligament fibroblasts, whereas its protein was observed in the matrices of dentin, enamel, and alveolar bone and in the cytoplasm of the aforementioned cells. The temporospatial expression profile revealed in this study indicates that FAM20C is an ECM protein that may play an important role in controlling the mineralization of bone and tooth. |
| doi_str_mv | 10.1369/jhc.2010.956565 |
| format | Article |
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No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developmental processes of bone and tooth. In the present study, we analyzed in detail the expression profile of FAM20C during osteogenesis and odontogenesis using ISH and IHC approaches. The specimens analyzed were mouse tissues spanning embryonic day 13.5 (E13.5) to postnatal 8 weeks. The earliest presence of FAM20C was observed at E14.5. During osteogenesis, FAM20C mRNA was detected in the chondrocytes and osteoblasts of the long bone, whereas its protein was observed in the extracellular matrix (ECM) of bone and in the cytoplasm of the chondrocytes, osteoblasts, and osteocytes. During odontogenesis, FAM20C mRNA was detected in the ameloblasts, odontoblasts, cementoblasts, and periodontal ligament fibroblasts, whereas its protein was observed in the matrices of dentin, enamel, and alveolar bone and in the cytoplasm of the aforementioned cells. The temporospatial expression profile revealed in this study indicates that FAM20C is an ECM protein that may play an important role in controlling the mineralization of bone and tooth.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1369/jhc.2010.956565</identifier><identifier>PMID: 20644212</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Antibody Specificity ; Bone and Bones - metabolism ; Calcium-Binding Proteins - analysis ; Calcium-Binding Proteins - genetics ; Calcium-Binding Proteins - immunology ; Calcium-Binding Proteins - metabolism ; Cell Line ; Extracellular Matrix Proteins - analysis ; Extracellular Matrix Proteins - genetics ; Extracellular Matrix Proteins - immunology ; Extracellular Matrix Proteins - metabolism ; Gene Expression Regulation, Developmental ; Humans ; Mice ; Odontogenesis - genetics ; Osteogenesis - genetics ; Recombinant Proteins - analysis ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinant Proteins - metabolism ; Tooth - growth & development ; Tooth - metabolism</subject><ispartof>The journal of histochemistry and cytochemistry, 2010-11, Vol.58 (11), p.957-967</ispartof><rights>2010 Authors</rights><rights>Copyright © 2010, Wang et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-a6bef0173ef1e4f363b896c2af7276305d14d2b638b96dc76fae1dc9f94104bb3</citedby><cites>FETCH-LOGICAL-c495t-a6bef0173ef1e4f363b896c2af7276305d14d2b638b96dc76fae1dc9f94104bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1369/jhc.2010.956565$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1369/jhc.2010.956565$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>230,314,780,784,885,21818,27923,27924,43620,43621</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20644212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xiaofang</creatorcontrib><creatorcontrib>Hao, Jianjun</creatorcontrib><creatorcontrib>Xie, Yixia</creatorcontrib><creatorcontrib>Sun, Yao</creatorcontrib><creatorcontrib>Hernandez, Brianda</creatorcontrib><creatorcontrib>Yamoah, Albert K.</creatorcontrib><creatorcontrib>Prasad, Monica</creatorcontrib><creatorcontrib>Zhu, Qinglin</creatorcontrib><creatorcontrib>Feng, Jian Q.</creatorcontrib><creatorcontrib>Qin, Chunlin</creatorcontrib><title>Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>Mutations in FAM20C were recently identified as the cause of lethal osteosclerotic bone dysplasia, which highlighted the important role of this molecule in biomineralization. No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developmental processes of bone and tooth. In the present study, we analyzed in detail the expression profile of FAM20C during osteogenesis and odontogenesis using ISH and IHC approaches. The specimens analyzed were mouse tissues spanning embryonic day 13.5 (E13.5) to postnatal 8 weeks. The earliest presence of FAM20C was observed at E14.5. During osteogenesis, FAM20C mRNA was detected in the chondrocytes and osteoblasts of the long bone, whereas its protein was observed in the extracellular matrix (ECM) of bone and in the cytoplasm of the chondrocytes, osteoblasts, and osteocytes. During odontogenesis, FAM20C mRNA was detected in the ameloblasts, odontoblasts, cementoblasts, and periodontal ligament fibroblasts, whereas its protein was observed in the matrices of dentin, enamel, and alveolar bone and in the cytoplasm of the aforementioned cells. The temporospatial expression profile revealed in this study indicates that FAM20C is an ECM protein that may play an important role in controlling the mineralization of bone and tooth.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Bone and Bones - metabolism</subject><subject>Calcium-Binding Proteins - analysis</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Calcium-Binding Proteins - immunology</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Cell Line</subject><subject>Extracellular Matrix Proteins - analysis</subject><subject>Extracellular Matrix Proteins - genetics</subject><subject>Extracellular Matrix Proteins - immunology</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Humans</subject><subject>Mice</subject><subject>Odontogenesis - genetics</subject><subject>Osteogenesis - genetics</subject><subject>Recombinant Proteins - analysis</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - metabolism</subject><subject>Tooth - growth & development</subject><subject>Tooth - metabolism</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFPwjAUhxujEUTP3sxuXhy0XdutFxOygJpAuOi56bZXGIEW12H0v7dkSPRgenjpe19_bT-EbgkekkTI0XpVDikOO8lFWGeoTzgnMceMnaM-xpTGocF66Mr7NcaEMZ5doh7FgjFKaB_lk89dA97XzkbORNPxnOI8qm3UriBa-BbcEiz42kfaVtGicrY9dQI_d3sP1-jC6I2Hm2MdoLfp5DV_jmeLp5d8PItLJnkba1GAwSRNwBBgJhFJkUlRUm1SmooE84qwihYiyQopqjIVRgOpSmkkI5gVRTJAj13ubl9soSrBto3eqF1Tb3XzpZyu1d-JrVdq6T4UlTwjSRYC7o8BjXvfg2_VtvYlbDbaQviISrnMUiZSHMhRR5aN874Bc7qFYHUwr4J5dTCvOvPhxN3vx534H9UBeOgAr5eg1m7f2CDr37xvdhWNVQ</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Wang, Xiaofang</creator><creator>Hao, Jianjun</creator><creator>Xie, Yixia</creator><creator>Sun, Yao</creator><creator>Hernandez, Brianda</creator><creator>Yamoah, Albert K.</creator><creator>Prasad, Monica</creator><creator>Zhu, Qinglin</creator><creator>Feng, Jian Q.</creator><creator>Qin, Chunlin</creator><general>SAGE Publications</general><general>Histochemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201011</creationdate><title>Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse</title><author>Wang, Xiaofang ; Hao, Jianjun ; Xie, Yixia ; Sun, Yao ; Hernandez, Brianda ; Yamoah, Albert K. ; Prasad, Monica ; Zhu, Qinglin ; Feng, Jian Q. ; Qin, Chunlin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-a6bef0173ef1e4f363b896c2af7276305d14d2b638b96dc76fae1dc9f94104bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Bone and Bones - metabolism</topic><topic>Calcium-Binding Proteins - analysis</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Calcium-Binding Proteins - immunology</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Cell Line</topic><topic>Extracellular Matrix Proteins - analysis</topic><topic>Extracellular Matrix Proteins - genetics</topic><topic>Extracellular Matrix Proteins - immunology</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Humans</topic><topic>Mice</topic><topic>Odontogenesis - genetics</topic><topic>Osteogenesis - genetics</topic><topic>Recombinant Proteins - analysis</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - metabolism</topic><topic>Tooth - growth & development</topic><topic>Tooth - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiaofang</creatorcontrib><creatorcontrib>Hao, Jianjun</creatorcontrib><creatorcontrib>Xie, Yixia</creatorcontrib><creatorcontrib>Sun, Yao</creatorcontrib><creatorcontrib>Hernandez, Brianda</creatorcontrib><creatorcontrib>Yamoah, Albert K.</creatorcontrib><creatorcontrib>Prasad, Monica</creatorcontrib><creatorcontrib>Zhu, Qinglin</creatorcontrib><creatorcontrib>Feng, Jian Q.</creatorcontrib><creatorcontrib>Qin, Chunlin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiaofang</au><au>Hao, Jianjun</au><au>Xie, Yixia</au><au>Sun, Yao</au><au>Hernandez, Brianda</au><au>Yamoah, Albert K.</au><au>Prasad, Monica</au><au>Zhu, Qinglin</au><au>Feng, Jian Q.</au><au>Qin, Chunlin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2010-11</date><risdate>2010</risdate><volume>58</volume><issue>11</issue><spage>957</spage><epage>967</epage><pages>957-967</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>Mutations in FAM20C were recently identified as the cause of lethal osteosclerotic bone dysplasia, which highlighted the important role of this molecule in biomineralization. No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developmental processes of bone and tooth. In the present study, we analyzed in detail the expression profile of FAM20C during osteogenesis and odontogenesis using ISH and IHC approaches. The specimens analyzed were mouse tissues spanning embryonic day 13.5 (E13.5) to postnatal 8 weeks. The earliest presence of FAM20C was observed at E14.5. During osteogenesis, FAM20C mRNA was detected in the chondrocytes and osteoblasts of the long bone, whereas its protein was observed in the extracellular matrix (ECM) of bone and in the cytoplasm of the chondrocytes, osteoblasts, and osteocytes. During odontogenesis, FAM20C mRNA was detected in the ameloblasts, odontoblasts, cementoblasts, and periodontal ligament fibroblasts, whereas its protein was observed in the matrices of dentin, enamel, and alveolar bone and in the cytoplasm of the aforementioned cells. 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| subjects | Animals Antibody Specificity Bone and Bones - metabolism Calcium-Binding Proteins - analysis Calcium-Binding Proteins - genetics Calcium-Binding Proteins - immunology Calcium-Binding Proteins - metabolism Cell Line Extracellular Matrix Proteins - analysis Extracellular Matrix Proteins - genetics Extracellular Matrix Proteins - immunology Extracellular Matrix Proteins - metabolism Gene Expression Regulation, Developmental Humans Mice Odontogenesis - genetics Osteogenesis - genetics Recombinant Proteins - analysis Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - metabolism Tooth - growth & development Tooth - metabolism |
| title | Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse |
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