Tissue tropism of the Musca domestica salivary gland hypertrophy virus
The tissue tropism of Musca domestica salivary gland hypertrophy virus (MdSGHV) infecting adult house flies was examined by transmission electron microscopy (TEM) and quantitative real-time PCR. TEM demonstrated that characteristic MdSGHV-induced nuclear and cellular hypertrophy was restricted to th...
Gespeichert in:
| Veröffentlicht in: | Virus research 2011-01, Vol.155 (1), p.20-27 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 27 |
|---|---|
| container_issue | 1 |
| container_start_page | 20 |
| container_title | Virus research |
| container_volume | 155 |
| creator | Lietze, Verena-Ulrike Salem, Tamer Z. Prompiboon, Pannipa Boucias, Drion G. |
| description | The tissue tropism of
Musca domestica salivary gland hypertrophy virus (MdSGHV) infecting adult house flies was examined by transmission electron microscopy (TEM) and quantitative real-time PCR. TEM demonstrated that characteristic MdSGHV-induced nuclear and cellular hypertrophy was restricted to the salivary glands. Both nucleocapsids and enveloped virions were present in salivary gland cells. In contrast, thin sections of midguts, ovaries, abdominal fat body, crops, air sacs and brains showed the presence of enveloped virions in vacuoles of tracheal cells associated with these tissues. However, no sites of viral morphogenesis were detected in the tracheal cells. Quantitative analysis of MdSGHV DNA and transcript titers revealed that viral DNA was present in all hemolymph and tissue samples collected from MdSGHV-infected flies. Average numbers of MdSGHV genome copies per 50
ng of DNA varied significantly between examined tissues and ranged from 3.83
×
10
8 (±3.75
×
10
7) in salivary gland samples to 7.98
×
10
5 (±2.91
×
10
5) in hemolymph samples. High levels of viral genome copies were detected in midgut, fat body and brain samples. Viral transcripts were present in all examined samples, and transcript abundance was also at the highest level in salivary glands and at the lowest level in hemolymph. However, over the range of different tissues that were analyzed, there was no correlation between estimated quantities of genome copies and viral transcripts. The function of viral transcripts in host tissues that do not show sites of viral morphogenesis remains to be elucidated. |
| doi_str_mv | 10.1016/j.virusres.2010.06.015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2956836</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168170210001991</els_id><sourcerecordid>860383991</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-a08ab3423be2d383402f19984e029d387bad38b6126e8e3eb27a53861b678c1b3</originalsourceid><addsrcrecordid>eNqFkUFv1DAQhS0EokvhL5TcOGUZ24ntXBCoorRSEQfas-U4k12vkvViJyvtv-8s21Zw6sW2xt88vZnH2AWHJQeuPm-W-5DmnDAvBVAR1BJ4_YotuNGi1FUjXrMFgabkGsQZe5fzBgCU1OotOxOgAKRpFuzqLuQ8YzGluAt5LGJfTGssfs7Zu6KLI-Yp0Cu7IexdOhSrwW27Yn3YYTq2rA_FXx_v2ZveDRk_PN7n7P7q-93ldXn768fN5bfb0tdCTaUD41pZCdmi6KSRFYieN42pEERDBd06OlvFhUKDEluhXS2N4q3SxvNWnrMvJ93d3I7YedxOyQ12l8JI7mx0wf7_sw1ru4p7K5paGalI4NOjQIp_ZprOjiF7HGgsjHO2RtFeZNPwl0nBK6O10kSqE-lTzBRJ_-yHgz2mZTf2KS17TMuCspQWNV78O81z21M8BHw8Ab2L1q1SyPb-NynUANzwuj66_HoikLa-D5hs9gG3HruQ0E-2i-ElFw_tkbRG</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>821487767</pqid></control><display><type>article</type><title>Tissue tropism of the Musca domestica salivary gland hypertrophy virus</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Lietze, Verena-Ulrike ; Salem, Tamer Z. ; Prompiboon, Pannipa ; Boucias, Drion G.</creator><creatorcontrib>Lietze, Verena-Ulrike ; Salem, Tamer Z. ; Prompiboon, Pannipa ; Boucias, Drion G.</creatorcontrib><description>The tissue tropism of
Musca domestica salivary gland hypertrophy virus (MdSGHV) infecting adult house flies was examined by transmission electron microscopy (TEM) and quantitative real-time PCR. TEM demonstrated that characteristic MdSGHV-induced nuclear and cellular hypertrophy was restricted to the salivary glands. Both nucleocapsids and enveloped virions were present in salivary gland cells. In contrast, thin sections of midguts, ovaries, abdominal fat body, crops, air sacs and brains showed the presence of enveloped virions in vacuoles of tracheal cells associated with these tissues. However, no sites of viral morphogenesis were detected in the tracheal cells. Quantitative analysis of MdSGHV DNA and transcript titers revealed that viral DNA was present in all hemolymph and tissue samples collected from MdSGHV-infected flies. Average numbers of MdSGHV genome copies per 50
ng of DNA varied significantly between examined tissues and ranged from 3.83
×
10
8 (±3.75
×
10
7) in salivary gland samples to 7.98
×
10
5 (±2.91
×
10
5) in hemolymph samples. High levels of viral genome copies were detected in midgut, fat body and brain samples. Viral transcripts were present in all examined samples, and transcript abundance was also at the highest level in salivary glands and at the lowest level in hemolymph. However, over the range of different tissues that were analyzed, there was no correlation between estimated quantities of genome copies and viral transcripts. The function of viral transcripts in host tissues that do not show sites of viral morphogenesis remains to be elucidated.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2010.06.015</identifier><identifier>PMID: 20600389</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>abdominal fat ; adults ; air sacs ; animal ovaries ; Animal Structures - ultrastructure ; Animal Structures - virology ; Animals ; brain ; crop (digestive system) ; DNA ; DNA Viruses - genetics ; DNA Viruses - physiology ; DNA Viruses - ultrastructure ; dsDNA virus ; fat body ; genome ; hemolymph ; Histology ; House fly ; Houseflies - virology ; hypertrophy ; Hytrosaviridae ; infection ; Insect virus ; Microscopy, Electron, Transmission ; midgut ; morphogenesis ; Musca domestica ; nucleocapsid ; Nucleocapsid - ultrastructure ; Polymerase Chain Reaction ; quantitative analysis ; quantitative polymerase chain reaction ; Real-time PCR ; salivary glands ; Salivary Glands - ultrastructure ; Salivary Glands - virology ; tissue tropism ; tracheae (invertebrates) ; transmission electron microscopy ; vacuoles ; Viral Tropism ; virion ; Virion - ultrastructure ; viruses</subject><ispartof>Virus research, 2011-01, Vol.155 (1), p.20-27</ispartof><rights>2010 Elsevier B.V.</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-a08ab3423be2d383402f19984e029d387bad38b6126e8e3eb27a53861b678c1b3</citedby><cites>FETCH-LOGICAL-c526t-a08ab3423be2d383402f19984e029d387bad38b6126e8e3eb27a53861b678c1b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virusres.2010.06.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20600389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lietze, Verena-Ulrike</creatorcontrib><creatorcontrib>Salem, Tamer Z.</creatorcontrib><creatorcontrib>Prompiboon, Pannipa</creatorcontrib><creatorcontrib>Boucias, Drion G.</creatorcontrib><title>Tissue tropism of the Musca domestica salivary gland hypertrophy virus</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>The tissue tropism of
Musca domestica salivary gland hypertrophy virus (MdSGHV) infecting adult house flies was examined by transmission electron microscopy (TEM) and quantitative real-time PCR. TEM demonstrated that characteristic MdSGHV-induced nuclear and cellular hypertrophy was restricted to the salivary glands. Both nucleocapsids and enveloped virions were present in salivary gland cells. In contrast, thin sections of midguts, ovaries, abdominal fat body, crops, air sacs and brains showed the presence of enveloped virions in vacuoles of tracheal cells associated with these tissues. However, no sites of viral morphogenesis were detected in the tracheal cells. Quantitative analysis of MdSGHV DNA and transcript titers revealed that viral DNA was present in all hemolymph and tissue samples collected from MdSGHV-infected flies. Average numbers of MdSGHV genome copies per 50
ng of DNA varied significantly between examined tissues and ranged from 3.83
×
10
8 (±3.75
×
10
7) in salivary gland samples to 7.98
×
10
5 (±2.91
×
10
5) in hemolymph samples. High levels of viral genome copies were detected in midgut, fat body and brain samples. Viral transcripts were present in all examined samples, and transcript abundance was also at the highest level in salivary glands and at the lowest level in hemolymph. However, over the range of different tissues that were analyzed, there was no correlation between estimated quantities of genome copies and viral transcripts. The function of viral transcripts in host tissues that do not show sites of viral morphogenesis remains to be elucidated.</description><subject>abdominal fat</subject><subject>adults</subject><subject>air sacs</subject><subject>animal ovaries</subject><subject>Animal Structures - ultrastructure</subject><subject>Animal Structures - virology</subject><subject>Animals</subject><subject>brain</subject><subject>crop (digestive system)</subject><subject>DNA</subject><subject>DNA Viruses - genetics</subject><subject>DNA Viruses - physiology</subject><subject>DNA Viruses - ultrastructure</subject><subject>dsDNA virus</subject><subject>fat body</subject><subject>genome</subject><subject>hemolymph</subject><subject>Histology</subject><subject>House fly</subject><subject>Houseflies - virology</subject><subject>hypertrophy</subject><subject>Hytrosaviridae</subject><subject>infection</subject><subject>Insect virus</subject><subject>Microscopy, Electron, Transmission</subject><subject>midgut</subject><subject>morphogenesis</subject><subject>Musca domestica</subject><subject>nucleocapsid</subject><subject>Nucleocapsid - ultrastructure</subject><subject>Polymerase Chain Reaction</subject><subject>quantitative analysis</subject><subject>quantitative polymerase chain reaction</subject><subject>Real-time PCR</subject><subject>salivary glands</subject><subject>Salivary Glands - ultrastructure</subject><subject>Salivary Glands - virology</subject><subject>tissue tropism</subject><subject>tracheae (invertebrates)</subject><subject>transmission electron microscopy</subject><subject>vacuoles</subject><subject>Viral Tropism</subject><subject>virion</subject><subject>Virion - ultrastructure</subject><subject>viruses</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EokvhL5TcOGUZ24ntXBCoorRSEQfas-U4k12vkvViJyvtv-8s21Zw6sW2xt88vZnH2AWHJQeuPm-W-5DmnDAvBVAR1BJ4_YotuNGi1FUjXrMFgabkGsQZe5fzBgCU1OotOxOgAKRpFuzqLuQ8YzGluAt5LGJfTGssfs7Zu6KLI-Yp0Cu7IexdOhSrwW27Yn3YYTq2rA_FXx_v2ZveDRk_PN7n7P7q-93ldXn768fN5bfb0tdCTaUD41pZCdmi6KSRFYieN42pEERDBd06OlvFhUKDEluhXS2N4q3SxvNWnrMvJ93d3I7YedxOyQ12l8JI7mx0wf7_sw1ru4p7K5paGalI4NOjQIp_ZprOjiF7HGgsjHO2RtFeZNPwl0nBK6O10kSqE-lTzBRJ_-yHgz2mZTf2KS17TMuCspQWNV78O81z21M8BHw8Ab2L1q1SyPb-NynUANzwuj66_HoikLa-D5hs9gG3HruQ0E-2i-ElFw_tkbRG</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Lietze, Verena-Ulrike</creator><creator>Salem, Tamer Z.</creator><creator>Prompiboon, Pannipa</creator><creator>Boucias, Drion G.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SS</scope><scope>7U9</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Tissue tropism of the Musca domestica salivary gland hypertrophy virus</title><author>Lietze, Verena-Ulrike ; Salem, Tamer Z. ; Prompiboon, Pannipa ; Boucias, Drion G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-a08ab3423be2d383402f19984e029d387bad38b6126e8e3eb27a53861b678c1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>abdominal fat</topic><topic>adults</topic><topic>air sacs</topic><topic>animal ovaries</topic><topic>Animal Structures - ultrastructure</topic><topic>Animal Structures - virology</topic><topic>Animals</topic><topic>brain</topic><topic>crop (digestive system)</topic><topic>DNA</topic><topic>DNA Viruses - genetics</topic><topic>DNA Viruses - physiology</topic><topic>DNA Viruses - ultrastructure</topic><topic>dsDNA virus</topic><topic>fat body</topic><topic>genome</topic><topic>hemolymph</topic><topic>Histology</topic><topic>House fly</topic><topic>Houseflies - virology</topic><topic>hypertrophy</topic><topic>Hytrosaviridae</topic><topic>infection</topic><topic>Insect virus</topic><topic>Microscopy, Electron, Transmission</topic><topic>midgut</topic><topic>morphogenesis</topic><topic>Musca domestica</topic><topic>nucleocapsid</topic><topic>Nucleocapsid - ultrastructure</topic><topic>Polymerase Chain Reaction</topic><topic>quantitative analysis</topic><topic>quantitative polymerase chain reaction</topic><topic>Real-time PCR</topic><topic>salivary glands</topic><topic>Salivary Glands - ultrastructure</topic><topic>Salivary Glands - virology</topic><topic>tissue tropism</topic><topic>tracheae (invertebrates)</topic><topic>transmission electron microscopy</topic><topic>vacuoles</topic><topic>Viral Tropism</topic><topic>virion</topic><topic>Virion - ultrastructure</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lietze, Verena-Ulrike</creatorcontrib><creatorcontrib>Salem, Tamer Z.</creatorcontrib><creatorcontrib>Prompiboon, Pannipa</creatorcontrib><creatorcontrib>Boucias, Drion G.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lietze, Verena-Ulrike</au><au>Salem, Tamer Z.</au><au>Prompiboon, Pannipa</au><au>Boucias, Drion G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue tropism of the Musca domestica salivary gland hypertrophy virus</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>155</volume><issue>1</issue><spage>20</spage><epage>27</epage><pages>20-27</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>The tissue tropism of
Musca domestica salivary gland hypertrophy virus (MdSGHV) infecting adult house flies was examined by transmission electron microscopy (TEM) and quantitative real-time PCR. TEM demonstrated that characteristic MdSGHV-induced nuclear and cellular hypertrophy was restricted to the salivary glands. Both nucleocapsids and enveloped virions were present in salivary gland cells. In contrast, thin sections of midguts, ovaries, abdominal fat body, crops, air sacs and brains showed the presence of enveloped virions in vacuoles of tracheal cells associated with these tissues. However, no sites of viral morphogenesis were detected in the tracheal cells. Quantitative analysis of MdSGHV DNA and transcript titers revealed that viral DNA was present in all hemolymph and tissue samples collected from MdSGHV-infected flies. Average numbers of MdSGHV genome copies per 50
ng of DNA varied significantly between examined tissues and ranged from 3.83
×
10
8 (±3.75
×
10
7) in salivary gland samples to 7.98
×
10
5 (±2.91
×
10
5) in hemolymph samples. High levels of viral genome copies were detected in midgut, fat body and brain samples. Viral transcripts were present in all examined samples, and transcript abundance was also at the highest level in salivary glands and at the lowest level in hemolymph. However, over the range of different tissues that were analyzed, there was no correlation between estimated quantities of genome copies and viral transcripts. The function of viral transcripts in host tissues that do not show sites of viral morphogenesis remains to be elucidated.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20600389</pmid><doi>10.1016/j.virusres.2010.06.015</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1702 |
| ispartof | Virus research, 2011-01, Vol.155 (1), p.20-27 |
| issn | 0168-1702 1872-7492 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2956836 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | abdominal fat adults air sacs animal ovaries Animal Structures - ultrastructure Animal Structures - virology Animals brain crop (digestive system) DNA DNA Viruses - genetics DNA Viruses - physiology DNA Viruses - ultrastructure dsDNA virus fat body genome hemolymph Histology House fly Houseflies - virology hypertrophy Hytrosaviridae infection Insect virus Microscopy, Electron, Transmission midgut morphogenesis Musca domestica nucleocapsid Nucleocapsid - ultrastructure Polymerase Chain Reaction quantitative analysis quantitative polymerase chain reaction Real-time PCR salivary glands Salivary Glands - ultrastructure Salivary Glands - virology tissue tropism tracheae (invertebrates) transmission electron microscopy vacuoles Viral Tropism virion Virion - ultrastructure viruses |
| title | Tissue tropism of the Musca domestica salivary gland hypertrophy virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T14%3A02%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tissue%20tropism%20of%20the%20Musca%20domestica%20salivary%20gland%20hypertrophy%20virus&rft.jtitle=Virus%20research&rft.au=Lietze,%20Verena-Ulrike&rft.date=2011-01-01&rft.volume=155&rft.issue=1&rft.spage=20&rft.epage=27&rft.pages=20-27&rft.issn=0168-1702&rft.eissn=1872-7492&rft_id=info:doi/10.1016/j.virusres.2010.06.015&rft_dat=%3Cproquest_pubme%3E860383991%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=821487767&rft_id=info:pmid/20600389&rft_els_id=S0168170210001991&rfr_iscdi=true |