Characterization and optimization of a red-shifted fluorescence polarization ADP detection assay

ATP depletion and ADP formation are generic detection methods used for the identification of kinase and other ATP-utilizing enzyme inhibitors in high-throughput screening campaigns. However, the most widely used nucleotide detection approaches require high ATP consumption rates or involve the use of...

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Veröffentlicht in:	Assay and drug development technologies 2009-02, Vol.7 (1), p.56-67
Hauptverfasser:	Kleman-Leyer, Karen M, Klink, Tony A, Kopp, Andrew L, Westermeyer, Thane A, Koeff, Mark D, Larson, Brad R, Worzella, Tracy J, Pinchard, Cori A, van de Kar, Sebastianus A T, Zaman, Guido J R, Hornberg, Jorrit J, Lowery, Robert G
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine diphosphate Adenosine Diphosphate - chemistry Adenosine Diphosphate - immunology Adenosine Diphosphate - metabolism Adenosine Triphosphate - chemistry Adenosine Triphosphate - metabolism Biological assay Buffers Cancer Care and treatment Chromatography, High Pressure Liquid Fluorescence Fluorescence Polarization Immunoassay - methods Indicators and Reagents Kinetics Methods Original Papers Peptide Library Properties Protein Kinases - metabolism Reference Standards
Online-Zugang:	Volltext
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