Tracer-based metabolomics: Concepts and practices
Tracer-based metabolomics is a systems biology tool that combines advances in tracer methodology for physiological studies, high throughput “-omics” technologies and constraint based modeling of metabolic networks. It is different from the commonly known metabolomics or metabonomics in that it is a...
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| Veröffentlicht in: | Clinical biochemistry 2010-11, Vol.43 (16), p.1269-1277 |
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| container_title | Clinical biochemistry |
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| creator | Paul Lee, W-N. Wahjudi, Paulin N. Xu, Jun Go, Vay Liang |
| description | Tracer-based metabolomics is a systems biology tool that combines advances in tracer methodology for physiological studies, high throughput “-omics” technologies and constraint based modeling of metabolic networks. It is different from the commonly known metabolomics or metabonomics in that it is a targeted approach based on a metabolic network model in cells. Because of its complexity, it is the least understood among the various “-omics.” In this review, the development of concepts and practices of tracer-based metabolomics is traced from the early application of radioactive isotopes in metabolic studies to the recent application of stable isotopes and isotopomer analysis using mass spectrometry; and from the modeling of biochemical reactions using flux analysis to the recent theoretical formulation of the constraint based modeling. How these newer experimental methods and concepts of constraint-based modeling approaches can be applied to metabolic studies is illustrated by examples of studies in determining metabolic responses of cells to pharmacological agents and nutrient environment changes. |
| doi_str_mv | 10.1016/j.clinbiochem.2010.07.027 |
| format | Article |
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How these newer experimental methods and concepts of constraint-based modeling approaches can be applied to metabolic studies is illustrated by examples of studies in determining metabolic responses of cells to pharmacological agents and nutrient environment changes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Isomerism</subject><subject>Isotope Labeling - methods</subject><subject>mass isotopomer analysis</subject><subject>Medical sciences</subject><subject>metabolomics</subject><subject>Metabolomics - methods</subject><subject>metabonomics</subject><subject>Miscellaneous. Technology</subject><subject>Models, Biological</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pharmacology. 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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Humans Investigative techniques, diagnostic techniques (general aspects) Isomerism Isotope Labeling - methods mass isotopomer analysis Medical sciences metabolomics Metabolomics - methods metabonomics Miscellaneous. Technology Models, Biological Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pharmacology. Drug treatments Phenotype phenotypic phase plane stable isotope tracer-based |
| title | Tracer-based metabolomics: Concepts and practices |
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