Growth Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli”
The present study was carried out to evaluate the effects of the water extract of Chinese medicine “Pingliu Keli” (PK) on human glioma cell viability and apoptosis and to investigate its mechanisms of action in SHG-44 cells. MTT assay showed that PK had a strong cytotoxic effect on SHG-44 cells. The...
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| creator | Cao, Peng Cai, Xueting Lu, Wuguang Zhou, Fei Huo, Jiege |
| description | The present study was carried out to evaluate the effects of the water extract of Chinese medicine “Pingliu Keli” (PK) on human glioma cell viability and apoptosis and to investigate its mechanisms of action in SHG-44 cells. MTT assay showed that PK had a strong cytotoxic effect on SHG-44 cells. The number of live cells was less than 20% after exposure to 90 μg/mL PK for 24 h. PK increased cytotoxicity of SHG-44 cells in a dose-dependent manner. PK caused arrest of SHG-44 cells in G1 phase at low concentration and in G2 phase at high concentration. The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 38% and 52% after treatment with 72 and 108 μg/mL PK, respectively. In addition, PK increased the expression of proapoptotic protein (Bax) and decreased antiapoptotic protein (Bcl-2), with a concomitant increase in the levels of cleaved caspase-3, cleaved caspase-9 and cleaved poly-ADP-ribose polymerase (PARP). These results suggest that PK has a significant apoptosis inducing effect on SHG-44 glioma cells in vitro and caspase-3 may act as a potential mediator in the process. |
| doi_str_mv | 10.1155/2011/958243 |
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MTT assay showed that PK had a strong cytotoxic effect on SHG-44 cells. The number of live cells was less than 20% after exposure to 90 μg/mL PK for 24 h. PK increased cytotoxicity of SHG-44 cells in a dose-dependent manner. PK caused arrest of SHG-44 cells in G1 phase at low concentration and in G2 phase at high concentration. The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 38% and 52% after treatment with 72 and 108 μg/mL PK, respectively. In addition, PK increased the expression of proapoptotic protein (Bax) and decreased antiapoptotic protein (Bcl-2), with a concomitant increase in the levels of cleaved caspase-3, cleaved caspase-9 and cleaved poly-ADP-ribose polymerase (PARP). These results suggest that PK has a significant apoptosis inducing effect on SHG-44 glioma cells in vitro and caspase-3 may act as a potential mediator in the process.</description><identifier>ISSN: 1741-427X</identifier><identifier>ISSN: 1741-4288</identifier><identifier>EISSN: 1741-4288</identifier><identifier>EISSN: 1741-427X</identifier><identifier>DOI: 10.1155/2011/958243</identifier><identifier>PMID: 20953401</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Adenosine diphosphate ; Apoptosis ; Arisaema heterophyllum ; BAX protein ; Bcl-2 protein ; Brain cancer ; Cancer therapies ; Caspase ; Caspase-3 ; Caspase-9 ; Cell cycle ; Chinese medicine ; Clinical trials ; Curcuma zedoaria ; Cytotoxicity ; Dendrobium officinale ; Deoxyribonucleic acid ; DNA ; Flow cytometry ; G1 phase ; G2 phase ; Glioma ; Glioma cells ; Herbal medicine ; Kinases ; Ligusticum chuanxiong ; Lycium chinense ; Medical prognosis ; Medical research ; Neurosurgery ; Penicillin ; Phytochemicals ; Poly(ADP-ribose) polymerase ; Ribose ; Signal transduction ; Tumors</subject><ispartof>Evidence-based complementary and alternative medicine, 2011-01, Vol.2011 (2011), p.1-9</ispartof><rights>Copyright © 2011 Peng Cao et al.</rights><rights>Copyright © 2011 Peng Cao et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2011 Peng Cao et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-90a8123160066139b86ab96bdc132489cf530b91c738dee826969f52fda874c13</citedby><cites>FETCH-LOGICAL-c526t-90a8123160066139b86ab96bdc132489cf530b91c738dee826969f52fda874c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2952337/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2952337/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20953401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Peng</creatorcontrib><creatorcontrib>Cai, Xueting</creatorcontrib><creatorcontrib>Lu, Wuguang</creatorcontrib><creatorcontrib>Zhou, Fei</creatorcontrib><creatorcontrib>Huo, Jiege</creatorcontrib><title>Growth Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli”</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>The present study was carried out to evaluate the effects of the water extract of Chinese medicine “Pingliu Keli” (PK) on human glioma cell viability and apoptosis and to investigate its mechanisms of action in SHG-44 cells. MTT assay showed that PK had a strong cytotoxic effect on SHG-44 cells. The number of live cells was less than 20% after exposure to 90 μg/mL PK for 24 h. PK increased cytotoxicity of SHG-44 cells in a dose-dependent manner. PK caused arrest of SHG-44 cells in G1 phase at low concentration and in G2 phase at high concentration. The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 38% and 52% after treatment with 72 and 108 μg/mL PK, respectively. In addition, PK increased the expression of proapoptotic protein (Bax) and decreased antiapoptotic protein (Bcl-2), with a concomitant increase in the levels of cleaved caspase-3, cleaved caspase-9 and cleaved poly-ADP-ribose polymerase (PARP). These results suggest that PK has a significant apoptosis inducing effect on SHG-44 glioma cells in vitro and caspase-3 may act as a potential mediator in the process.</description><subject>Adenosine diphosphate</subject><subject>Apoptosis</subject><subject>Arisaema heterophyllum</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>Brain cancer</subject><subject>Cancer therapies</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Caspase-9</subject><subject>Cell cycle</subject><subject>Chinese medicine</subject><subject>Clinical trials</subject><subject>Curcuma zedoaria</subject><subject>Cytotoxicity</subject><subject>Dendrobium officinale</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Flow cytometry</subject><subject>G1 phase</subject><subject>G2 phase</subject><subject>Glioma</subject><subject>Glioma cells</subject><subject>Herbal medicine</subject><subject>Kinases</subject><subject>Ligusticum 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Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli”</title><author>Cao, Peng ; Cai, Xueting ; Lu, Wuguang ; Zhou, Fei ; Huo, Jiege</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-90a8123160066139b86ab96bdc132489cf530b91c738dee826969f52fda874c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenosine diphosphate</topic><topic>Apoptosis</topic><topic>Arisaema heterophyllum</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>Brain cancer</topic><topic>Cancer therapies</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Caspase-9</topic><topic>Cell cycle</topic><topic>Chinese medicine</topic><topic>Clinical trials</topic><topic>Curcuma zedoaria</topic><topic>Cytotoxicity</topic><topic>Dendrobium officinale</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Flow 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medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>2011</volume><issue>2011</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1741-427X</issn><issn>1741-4288</issn><eissn>1741-4288</eissn><eissn>1741-427X</eissn><abstract>The present study was carried out to evaluate the effects of the water extract of Chinese medicine “Pingliu Keli” (PK) on human glioma cell viability and apoptosis and to investigate its mechanisms of action in SHG-44 cells. MTT assay showed that PK had a strong cytotoxic effect on SHG-44 cells. The number of live cells was less than 20% after exposure to 90 μg/mL PK for 24 h. PK increased cytotoxicity of SHG-44 cells in a dose-dependent manner. PK caused arrest of SHG-44 cells in G1 phase at low concentration and in G2 phase at high concentration. The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 38% and 52% after treatment with 72 and 108 μg/mL PK, respectively. In addition, PK increased the expression of proapoptotic protein (Bax) and decreased antiapoptotic protein (Bcl-2), with a concomitant increase in the levels of cleaved caspase-3, cleaved caspase-9 and cleaved poly-ADP-ribose polymerase (PARP). These results suggest that PK has a significant apoptosis inducing effect on SHG-44 glioma cells in vitro and caspase-3 may act as a potential mediator in the process.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>20953401</pmid><doi>10.1155/2011/958243</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Adenosine diphosphate Apoptosis Arisaema heterophyllum BAX protein Bcl-2 protein Brain cancer Cancer therapies Caspase Caspase-3 Caspase-9 Cell cycle Chinese medicine Clinical trials Curcuma zedoaria Cytotoxicity Dendrobium officinale Deoxyribonucleic acid DNA Flow cytometry G1 phase G2 phase Glioma Glioma cells Herbal medicine Kinases Ligusticum chuanxiong Lycium chinense Medical prognosis Medical research Neurosurgery Penicillin Phytochemicals Poly(ADP-ribose) polymerase Ribose Signal transduction Tumors |
| title | Growth Inhibition and Induction of Apoptosis in SHG-44 Glioma Cells by Chinese Medicine Formula “Pingliu Keli” |
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