Production of Anti-carbohydrate Antibodies by Phage Display Technologies: POTENTIAL IMPAIRMENT OF CELL GROWTH AS A RESULT OF ENDOGENOUS EXPRESSION

Anti-mannotriose (Man3) antibodies were previously isolated from a Keio phage library displaying human single chain variable fragments (scFvs) using a neoglycolipid, Man3- dipalmitoylphosphatidylethanolamine. Of three genes constructed, the 5A3 clone was expressed in mouse myeloma NS0 cells as a con...

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Veröffentlicht in:	The Journal of biological chemistry 2010-10, Vol.285 (40), p.30587-30597
Hauptverfasser:	Yuasa, Noriyuki, Zhang, Wei, Goto, Tomohiro, Sakaue, Hiroyuki, Matsumoto-Takasaki, Ayano, Kimura, Miyo, Ohshima, Hiroya, Tsuchida, Yasunobu, Koizumi, Tomoyuki, Sakai, Keiko, Kojima, Takumi, Yamamoto, Kazuo, Nakata, Munehiro, Fujita-Yamaguchi, Yoko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Cell Biology Cell Line, Tumor Chlorocebus aethiops COS Cells Endoplasmic Reticulum - genetics Endoplasmic Reticulum - immunology Endoplasmic Reticulum - metabolism Gene Expression Glycobiology and Extracellular Matrices Humans Immunoglobulin G - biosynthesis Immunoglobulin G - genetics Immunoglobulin G - immunology Immunoglobulin G - isolation & purification Mice Single-Chain Antibodies - biosynthesis Single-Chain Antibodies - genetics Single-Chain Antibodies - immunology Single-Chain Antibodies - isolation & purification Trisaccharides - immunology
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