Effective induction of protective systemic immunity with nasally administered vaccines adjuvanted with IL-1
Abstract IL-1α and IL-1β were evaluated for their ability to provide adjuvant activity for the induction of serum antibody responses when nasally administered with protein antigens in mice and rabbits. In mice, intranasal (i.n.) immunization with pneumococcal surface protein A (PspA) or tetanus toxo...
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| Veröffentlicht in: | Vaccine 2010-10, Vol.28 (42), p.6901-6914 |
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| creator | Gwinn, William M Kirwan, Shaun M Wang, Sheena H Ashcraft, Kathleen A Sparks, Neil L Doil, Catherine R Tlusty, Tom G Casey, Leslie S Hollingshead, Susan K Briles, David E Dondero, Richard S Hickey, Anthony J Foster, W. Michael Staats, Herman F |
| description | Abstract IL-1α and IL-1β were evaluated for their ability to provide adjuvant activity for the induction of serum antibody responses when nasally administered with protein antigens in mice and rabbits. In mice, intranasal (i.n.) immunization with pneumococcal surface protein A (PspA) or tetanus toxoid (TT) combined with IL-1β induced protective immunity that was equivalent to that induced by parenteral immunization. Nasal immunization of awake (i.e., not anesthetized) rabbits with IL-1-adjuvanted vaccines induced highly variable serum antibody responses and was not as effective as parenteral immunization for the induction of antigen-specific serum IgG. However, i.n. immunization of deeply anesthetized rabbits with rPA + IL-1α consistently induced rPA-specific serum IgG ELISA titers that were not significantly different than those induced by intramuscular (IM) immunization with rPA + alum although lethal toxin-neutralizing titers induced by nasal immunization were lower than those induced by IM immunization. Gamma scintigraphy demonstrated that the enhanced immunogenicity of nasal immunization in anesthetized rabbits correlated with an increased nasal retention of i.n. delivered non-permeable radio-labeled colloidal particles. Our results demonstrate that, in mice, IL-1 is an effective adjuvant for nasally administered vaccines for the induction of protective systemic immunity and that in non-rodent species, effective induction of systemic immunity with nasally administered vaccines may require formulations that ensure adequate retention of the vaccine within the nasal cavity. |
| doi_str_mv | 10.1016/j.vaccine.2010.08.006 |
| format | Article |
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Michael ; Staats, Herman F</creator><creatorcontrib>Gwinn, William M ; Kirwan, Shaun M ; Wang, Sheena H ; Ashcraft, Kathleen A ; Sparks, Neil L ; Doil, Catherine R ; Tlusty, Tom G ; Casey, Leslie S ; Hollingshead, Susan K ; Briles, David E ; Dondero, Richard S ; Hickey, Anthony J ; Foster, W. Michael ; Staats, Herman F</creatorcontrib><description>Abstract IL-1α and IL-1β were evaluated for their ability to provide adjuvant activity for the induction of serum antibody responses when nasally administered with protein antigens in mice and rabbits. In mice, intranasal (i.n.) immunization with pneumococcal surface protein A (PspA) or tetanus toxoid (TT) combined with IL-1β induced protective immunity that was equivalent to that induced by parenteral immunization. Nasal immunization of awake (i.e., not anesthetized) rabbits with IL-1-adjuvanted vaccines induced highly variable serum antibody responses and was not as effective as parenteral immunization for the induction of antigen-specific serum IgG. However, i.n. immunization of deeply anesthetized rabbits with rPA + IL-1α consistently induced rPA-specific serum IgG ELISA titers that were not significantly different than those induced by intramuscular (IM) immunization with rPA + alum although lethal toxin-neutralizing titers induced by nasal immunization were lower than those induced by IM immunization. Gamma scintigraphy demonstrated that the enhanced immunogenicity of nasal immunization in anesthetized rabbits correlated with an increased nasal retention of i.n. delivered non-permeable radio-labeled colloidal particles. Our results demonstrate that, in mice, IL-1 is an effective adjuvant for nasally administered vaccines for the induction of protective systemic immunity and that in non-rodent species, effective induction of systemic immunity with nasally administered vaccines may require formulations that ensure adequate retention of the vaccine within the nasal cavity.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2010.08.006</identifier><identifier>PMID: 20723629</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject><![CDATA[Adjuvant ; Adjuvants, Immunologic - administration & dosage ; Administration, Intranasal ; Allergy and Immunology ; Animals ; Antibodies, Bacterial - blood ; Antigens, Bacterial - administration & dosage ; Antigens, Bacterial - immunology ; Applied microbiology ; Bacterial Proteins - administration & dosage ; Bacterial Proteins - immunology ; Bacterial Toxins - administration & dosage ; Bacterial Toxins - immunology ; Bacterial Vaccines - administration & dosage ; Bacterial Vaccines - immunology ; Biological and medical sciences ; FDA approval ; Female ; Fundamental and applied biological sciences. Psychology ; Immune system ; Immunization ; Immunization - methods ; Immunogenicity ; Immunoglobulin G - blood ; Interleukin 1 ; Interleukin-1alpha - immunology ; Interleukin-1beta - immunology ; Laboratory animals ; Methods ; Mice ; Mice, Inbred BALB C ; Microbiology ; Nasal ; Neutralization Tests ; Rabbits ; Radionuclide Imaging ; Retention ; Streptococcus infections ; Streptococcus pneumoniae - immunology ; Tetanus ; Tetanus Toxoid - administration & dosage ; Tetanus Toxoid - immunology ; Toxins ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)]]></subject><ispartof>Vaccine, 2010-10, Vol.28 (42), p.6901-6914</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Oct 4, 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c579t-390bb06afa0a58675137deaa403298fa8c081b8ccfb14cb13b1d084b5ce4ca063</citedby><cites>FETCH-LOGICAL-c579t-390bb06afa0a58675137deaa403298fa8c081b8ccfb14cb13b1d084b5ce4ca063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X10011151$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23296705$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20723629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gwinn, William M</creatorcontrib><creatorcontrib>Kirwan, Shaun M</creatorcontrib><creatorcontrib>Wang, Sheena H</creatorcontrib><creatorcontrib>Ashcraft, Kathleen A</creatorcontrib><creatorcontrib>Sparks, Neil L</creatorcontrib><creatorcontrib>Doil, Catherine R</creatorcontrib><creatorcontrib>Tlusty, Tom G</creatorcontrib><creatorcontrib>Casey, Leslie S</creatorcontrib><creatorcontrib>Hollingshead, Susan K</creatorcontrib><creatorcontrib>Briles, David E</creatorcontrib><creatorcontrib>Dondero, Richard S</creatorcontrib><creatorcontrib>Hickey, Anthony J</creatorcontrib><creatorcontrib>Foster, W. Michael</creatorcontrib><creatorcontrib>Staats, Herman F</creatorcontrib><title>Effective induction of protective systemic immunity with nasally administered vaccines adjuvanted with IL-1</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract IL-1α and IL-1β were evaluated for their ability to provide adjuvant activity for the induction of serum antibody responses when nasally administered with protein antigens in mice and rabbits. In mice, intranasal (i.n.) immunization with pneumococcal surface protein A (PspA) or tetanus toxoid (TT) combined with IL-1β induced protective immunity that was equivalent to that induced by parenteral immunization. Nasal immunization of awake (i.e., not anesthetized) rabbits with IL-1-adjuvanted vaccines induced highly variable serum antibody responses and was not as effective as parenteral immunization for the induction of antigen-specific serum IgG. However, i.n. immunization of deeply anesthetized rabbits with rPA + IL-1α consistently induced rPA-specific serum IgG ELISA titers that were not significantly different than those induced by intramuscular (IM) immunization with rPA + alum although lethal toxin-neutralizing titers induced by nasal immunization were lower than those induced by IM immunization. Gamma scintigraphy demonstrated that the enhanced immunogenicity of nasal immunization in anesthetized rabbits correlated with an increased nasal retention of i.n. delivered non-permeable radio-labeled colloidal particles. Our results demonstrate that, in mice, IL-1 is an effective adjuvant for nasally administered vaccines for the induction of protective systemic immunity and that in non-rodent species, effective induction of systemic immunity with nasally administered vaccines may require formulations that ensure adequate retention of the vaccine within the nasal cavity.</description><subject>Adjuvant</subject><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Administration, Intranasal</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - administration & dosage</subject><subject>Antigens, Bacterial - immunology</subject><subject>Applied microbiology</subject><subject>Bacterial Proteins - administration & dosage</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Toxins - administration & dosage</subject><subject>Bacterial Toxins - immunology</subject><subject>Bacterial Vaccines - administration & dosage</subject><subject>Bacterial Vaccines - immunology</subject><subject>Biological and medical sciences</subject><subject>FDA approval</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Immunization - methods</subject><subject>Immunogenicity</subject><subject>Immunoglobulin G - blood</subject><subject>Interleukin 1</subject><subject>Interleukin-1alpha - immunology</subject><subject>Interleukin-1beta - immunology</subject><subject>Laboratory animals</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Nasal</subject><subject>Neutralization Tests</subject><subject>Rabbits</subject><subject>Radionuclide Imaging</subject><subject>Retention</subject><subject>Streptococcus infections</subject><subject>Streptococcus pneumoniae - immunology</subject><subject>Tetanus</subject><subject>Tetanus Toxoid - administration & dosage</subject><subject>Tetanus Toxoid - immunology</subject><subject>Toxins</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFktFv1SAUxonRuOv0T9A0McanXg9QKH3ZYpapS27ig5r4Riiljq6FK7TX9L-X7tZN9-IT5PDj4zvnA6GXGLYYMH_XbQ9Ka-vMlkCqgdgC8Edog0VJc8KweIw2QHiRFxi-n6BnMXYAwCiunqITAiWhnFQbdHPZtkaP9mAy65op7bzLfJvtgx_XepzjaAarMzsMk7PjnP2y43XmVFR9P2eqGayzCQmmyVZPMVW76aDcmGq39NUux8_Rk1b10bxY11P07cPl14tP-e7zx6uL97tcs7Iac1pBXQNXrQLFBC8ZpmVjlCqAkkq0SmgQuBZatzUudI1pjRsQRc20KbQCTk_R2VF3P9WDabRxY1C93Ac7qDBLr6z898TZa_nDHySpCsooSQJvV4Hgf04mjnKwUZu-V874KcqSMcwZVCyRrx-QnZ-CS91JXFQlIZyzRY8dKR18jMG0d14wyCVN2cl1cnJJU4KQcNvIq78bubv1J74EvFkBFbXq26CctvGeSwPjJSw2z4-cSWM_WBNk1NY4bRobUsqy8fa_Vs4eKOg-xZ4evTGzifddy0gkyC_L11t-HgbAGKcIfwPP8NfU</recordid><startdate>20101004</startdate><enddate>20101004</enddate><creator>Gwinn, William M</creator><creator>Kirwan, Shaun M</creator><creator>Wang, Sheena H</creator><creator>Ashcraft, Kathleen A</creator><creator>Sparks, Neil L</creator><creator>Doil, Catherine R</creator><creator>Tlusty, Tom G</creator><creator>Casey, Leslie S</creator><creator>Hollingshead, Susan K</creator><creator>Briles, David E</creator><creator>Dondero, Richard S</creator><creator>Hickey, Anthony J</creator><creator>Foster, W. 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Michael ; Staats, Herman F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-390bb06afa0a58675137deaa403298fa8c081b8ccfb14cb13b1d084b5ce4ca063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adjuvant</topic><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Administration, Intranasal</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial - administration & dosage</topic><topic>Antigens, Bacterial - immunology</topic><topic>Applied microbiology</topic><topic>Bacterial Proteins - administration & dosage</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Toxins - administration & dosage</topic><topic>Bacterial Toxins - immunology</topic><topic>Bacterial Vaccines - administration & dosage</topic><topic>Bacterial Vaccines - immunology</topic><topic>Biological and medical sciences</topic><topic>FDA approval</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Immunization - methods</topic><topic>Immunogenicity</topic><topic>Immunoglobulin G - blood</topic><topic>Interleukin 1</topic><topic>Interleukin-1alpha - immunology</topic><topic>Interleukin-1beta - immunology</topic><topic>Laboratory animals</topic><topic>Methods</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Nasal</topic><topic>Neutralization Tests</topic><topic>Rabbits</topic><topic>Radionuclide Imaging</topic><topic>Retention</topic><topic>Streptococcus infections</topic><topic>Streptococcus pneumoniae - immunology</topic><topic>Tetanus</topic><topic>Tetanus Toxoid - administration & dosage</topic><topic>Tetanus Toxoid - immunology</topic><topic>Toxins</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gwinn, William M</creatorcontrib><creatorcontrib>Kirwan, Shaun M</creatorcontrib><creatorcontrib>Wang, Sheena H</creatorcontrib><creatorcontrib>Ashcraft, Kathleen A</creatorcontrib><creatorcontrib>Sparks, Neil L</creatorcontrib><creatorcontrib>Doil, Catherine R</creatorcontrib><creatorcontrib>Tlusty, Tom G</creatorcontrib><creatorcontrib>Casey, Leslie S</creatorcontrib><creatorcontrib>Hollingshead, Susan K</creatorcontrib><creatorcontrib>Briles, David E</creatorcontrib><creatorcontrib>Dondero, Richard S</creatorcontrib><creatorcontrib>Hickey, Anthony J</creatorcontrib><creatorcontrib>Foster, W. 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Michael</au><au>Staats, Herman F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effective induction of protective systemic immunity with nasally administered vaccines adjuvanted with IL-1</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2010-10-04</date><risdate>2010</risdate><volume>28</volume><issue>42</issue><spage>6901</spage><epage>6914</epage><pages>6901-6914</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract IL-1α and IL-1β were evaluated for their ability to provide adjuvant activity for the induction of serum antibody responses when nasally administered with protein antigens in mice and rabbits. In mice, intranasal (i.n.) immunization with pneumococcal surface protein A (PspA) or tetanus toxoid (TT) combined with IL-1β induced protective immunity that was equivalent to that induced by parenteral immunization. Nasal immunization of awake (i.e., not anesthetized) rabbits with IL-1-adjuvanted vaccines induced highly variable serum antibody responses and was not as effective as parenteral immunization for the induction of antigen-specific serum IgG. However, i.n. immunization of deeply anesthetized rabbits with rPA + IL-1α consistently induced rPA-specific serum IgG ELISA titers that were not significantly different than those induced by intramuscular (IM) immunization with rPA + alum although lethal toxin-neutralizing titers induced by nasal immunization were lower than those induced by IM immunization. Gamma scintigraphy demonstrated that the enhanced immunogenicity of nasal immunization in anesthetized rabbits correlated with an increased nasal retention of i.n. delivered non-permeable radio-labeled colloidal particles. Our results demonstrate that, in mice, IL-1 is an effective adjuvant for nasally administered vaccines for the induction of protective systemic immunity and that in non-rodent species, effective induction of systemic immunity with nasally administered vaccines may require formulations that ensure adequate retention of the vaccine within the nasal cavity.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>20723629</pmid><doi>10.1016/j.vaccine.2010.08.006</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2010-10, Vol.28 (42), p.6901-6914 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2943532 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adjuvant Adjuvants, Immunologic - administration & dosage Administration, Intranasal Allergy and Immunology Animals Antibodies, Bacterial - blood Antigens, Bacterial - administration & dosage Antigens, Bacterial - immunology Applied microbiology Bacterial Proteins - administration & dosage Bacterial Proteins - immunology Bacterial Toxins - administration & dosage Bacterial Toxins - immunology Bacterial Vaccines - administration & dosage Bacterial Vaccines - immunology Biological and medical sciences FDA approval Female Fundamental and applied biological sciences. Psychology Immune system Immunization Immunization - methods Immunogenicity Immunoglobulin G - blood Interleukin 1 Interleukin-1alpha - immunology Interleukin-1beta - immunology Laboratory animals Methods Mice Mice, Inbred BALB C Microbiology Nasal Neutralization Tests Rabbits Radionuclide Imaging Retention Streptococcus infections Streptococcus pneumoniae - immunology Tetanus Tetanus Toxoid - administration & dosage Tetanus Toxoid - immunology Toxins Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Effective induction of protective systemic immunity with nasally administered vaccines adjuvanted with IL-1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T01%3A14%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effective%20induction%20of%20protective%20systemic%20immunity%20with%20nasally%20administered%20vaccines%20adjuvanted%20with%20IL-1&rft.jtitle=Vaccine&rft.au=Gwinn,%20William%20M&rft.date=2010-10-04&rft.volume=28&rft.issue=42&rft.spage=6901&rft.epage=6914&rft.pages=6901-6914&rft.issn=0264-410X&rft.eissn=1873-2518&rft.coden=VACCDE&rft_id=info:doi/10.1016/j.vaccine.2010.08.006&rft_dat=%3Cproquest_pubme%3E3216318551%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1497226652&rft_id=info:pmid/20723629&rft_els_id=S0264410X10011151&rfr_iscdi=true |