Fresh Frozen Plasma Increases Adhesion Molecule Expression on Human Pulmonary Endothelial Cells
Background Current blood banking practices allow fresh frozen plasma (FFP) to be thawed and then stored for 5 d between 1 and 6 °C. We hypothesized that aged plasma (d 5 FFP) would be pro-inflammatory to the endothelium compared with fresh plasma (d 0 FFP). Materials and Methods Human pulmonary endo...
Gespeichert in:
| Veröffentlicht in: | The Journal of surgical research 2010-10, Vol.163 (2), p.317-322 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 322 |
|---|---|
| container_issue | 2 |
| container_start_page | 317 |
| container_title | The Journal of surgical research |
| container_volume | 163 |
| creator | Letourneau, Phillip A., M.D Pati, Shibani, M.D., Ph.D Gerber, Michael H., B.S Jimenez, Fernando, M.S Holcomb, John B., M.D |
| description | Background Current blood banking practices allow fresh frozen plasma (FFP) to be thawed and then stored for 5 d between 1 and 6 °C. We hypothesized that aged plasma (d 5 FFP) would be pro-inflammatory to the endothelium compared with fresh plasma (d 0 FFP). Materials and Methods Human pulmonary endothelial cells (PECs) were treated with (1) media, (2) media + lipopolysaccharide (LPS), (3) lactated Ringer's (LR), (4) LR + LPS, (5) d 0 FFP, (6) d 0 FFP + LPS, (7) d 5 FFP, and (8) d 5 FFP + LPS. After a 24 h incubation, the PECs were stained with antibodies for I-CAM, V-CAM, P-selectin, and E-selectin. The cells were subsequently analyzed by flow cytometry. Results In both PEC groups treated with FFP and stimulated with LPS, I-CAM, V-CAM, P-selectin, and E-selectin were significantly up-regulated compared with LR when stimulated by LPS. Conclusion FFP at both ages significantly increased expression of four different adhesion molecules compared with LR in PECs. This may represent a possible mechanism for increased leukocyte binding on the endothelium as a result of FFP transfusion. |
| doi_str_mv | 10.1016/j.jss.2010.04.049 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2943032</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0022480410004361</els_id><sourcerecordid>755172319</sourcerecordid><originalsourceid>FETCH-LOGICAL-c535t-a80145016855045f552e36bb9a1e5d83d8ba28877e2b799cfbb576fa0d84cbfd3</originalsourceid><addsrcrecordid>eNp9Uk1v1DAQjRCIbgs_gAvKBfWUxR9xEgupUrXapZWKQALOluNMWC-OvXiSivLr67BL-TggjWSN_d6bGb_JsheULCmh1evdcoe4ZCTlpEwhH2ULSqQomqrmj7MFIYwVZUPKk-wUcUdSLmv-NDthREjJKFlkahMBt_kmhh_g8w9O46Dza28iaATML7stoA0-fxccmMlBvv6-T4yfdymupkEn2uSG4HW8y9e-C-MWnNUuX4Fz-Cx70muH8Px4nmWfN-tPq6vi5v3b69XlTWEEF2OhG0JLkWZqhCCl6IVgwKu2lZqC6BreNa1mTVPXwNpaStO3rairXpOuKU3bd_wsuzjo7qd2gM6AH6N2ah_tkPpSQVv194u3W_Ul3ComS044SwLnR4EYvk2AoxosmjSC9hAmVLUQtGacyoSkB6SJATFC_1CFEjX7onYq-aJmXxQpU8ycl3-298D4ZUQCvDoCNBrt-qi9sfgbx1lFGS8T7s0BB-kzby1EhcaCN9DZCGZUXbD_bePiH7Zx1ttU8CvcAe7CFH1ySVGFTBH1cV6geX9oWp2SV5TfA6XywV4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>755172319</pqid></control><display><type>article</type><title>Fresh Frozen Plasma Increases Adhesion Molecule Expression on Human Pulmonary Endothelial Cells</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Letourneau, Phillip A., M.D ; Pati, Shibani, M.D., Ph.D ; Gerber, Michael H., B.S ; Jimenez, Fernando, M.S ; Holcomb, John B., M.D</creator><creatorcontrib>Letourneau, Phillip A., M.D ; Pati, Shibani, M.D., Ph.D ; Gerber, Michael H., B.S ; Jimenez, Fernando, M.S ; Holcomb, John B., M.D</creatorcontrib><description>Background Current blood banking practices allow fresh frozen plasma (FFP) to be thawed and then stored for 5 d between 1 and 6 °C. We hypothesized that aged plasma (d 5 FFP) would be pro-inflammatory to the endothelium compared with fresh plasma (d 0 FFP). Materials and Methods Human pulmonary endothelial cells (PECs) were treated with (1) media, (2) media + lipopolysaccharide (LPS), (3) lactated Ringer's (LR), (4) LR + LPS, (5) d 0 FFP, (6) d 0 FFP + LPS, (7) d 5 FFP, and (8) d 5 FFP + LPS. After a 24 h incubation, the PECs were stained with antibodies for I-CAM, V-CAM, P-selectin, and E-selectin. The cells were subsequently analyzed by flow cytometry. Results In both PEC groups treated with FFP and stimulated with LPS, I-CAM, V-CAM, P-selectin, and E-selectin were significantly up-regulated compared with LR when stimulated by LPS. Conclusion FFP at both ages significantly increased expression of four different adhesion molecules compared with LR in PECs. This may represent a possible mechanism for increased leukocyte binding on the endothelium as a result of FFP transfusion.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2010.04.049</identifier><identifier>PMID: 20599210</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cell Adhesion Molecules - analysis ; Cells, Cultured ; E-Selectin - analysis ; Endothelial Cells - chemistry ; endothelium ; General aspects ; Humans ; Intercellular Adhesion Molecule-1 - analysis ; Lipopolysaccharides - pharmacology ; Lung - chemistry ; Medical sciences ; P-Selectin - analysis ; plasma ; Plasma - physiology ; Respiratory Distress Syndrome, Adult - etiology ; resuscitation ; Surgery ; trauma ; Vascular Cell Adhesion Molecule-1 - analysis</subject><ispartof>The Journal of surgical research, 2010-10, Vol.163 (2), p.317-322</ispartof><rights>Elsevier Inc.</rights><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><rights>2010 Elsevier Inc. All rights reserved. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-a80145016855045f552e36bb9a1e5d83d8ba28877e2b799cfbb576fa0d84cbfd3</citedby><cites>FETCH-LOGICAL-c535t-a80145016855045f552e36bb9a1e5d83d8ba28877e2b799cfbb576fa0d84cbfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jss.2010.04.049$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23261234$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20599210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Letourneau, Phillip A., M.D</creatorcontrib><creatorcontrib>Pati, Shibani, M.D., Ph.D</creatorcontrib><creatorcontrib>Gerber, Michael H., B.S</creatorcontrib><creatorcontrib>Jimenez, Fernando, M.S</creatorcontrib><creatorcontrib>Holcomb, John B., M.D</creatorcontrib><title>Fresh Frozen Plasma Increases Adhesion Molecule Expression on Human Pulmonary Endothelial Cells</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Background Current blood banking practices allow fresh frozen plasma (FFP) to be thawed and then stored for 5 d between 1 and 6 °C. We hypothesized that aged plasma (d 5 FFP) would be pro-inflammatory to the endothelium compared with fresh plasma (d 0 FFP). Materials and Methods Human pulmonary endothelial cells (PECs) were treated with (1) media, (2) media + lipopolysaccharide (LPS), (3) lactated Ringer's (LR), (4) LR + LPS, (5) d 0 FFP, (6) d 0 FFP + LPS, (7) d 5 FFP, and (8) d 5 FFP + LPS. After a 24 h incubation, the PECs were stained with antibodies for I-CAM, V-CAM, P-selectin, and E-selectin. The cells were subsequently analyzed by flow cytometry. Results In both PEC groups treated with FFP and stimulated with LPS, I-CAM, V-CAM, P-selectin, and E-selectin were significantly up-regulated compared with LR when stimulated by LPS. Conclusion FFP at both ages significantly increased expression of four different adhesion molecules compared with LR in PECs. This may represent a possible mechanism for increased leukocyte binding on the endothelium as a result of FFP transfusion.</description><subject>Biological and medical sciences</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cells, Cultured</subject><subject>E-Selectin - analysis</subject><subject>Endothelial Cells - chemistry</subject><subject>endothelium</subject><subject>General aspects</subject><subject>Humans</subject><subject>Intercellular Adhesion Molecule-1 - analysis</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lung - chemistry</subject><subject>Medical sciences</subject><subject>P-Selectin - analysis</subject><subject>plasma</subject><subject>Plasma - physiology</subject><subject>Respiratory Distress Syndrome, Adult - etiology</subject><subject>resuscitation</subject><subject>Surgery</subject><subject>trauma</subject><subject>Vascular Cell Adhesion Molecule-1 - analysis</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uk1v1DAQjRCIbgs_gAvKBfWUxR9xEgupUrXapZWKQALOluNMWC-OvXiSivLr67BL-TggjWSN_d6bGb_JsheULCmh1evdcoe4ZCTlpEwhH2ULSqQomqrmj7MFIYwVZUPKk-wUcUdSLmv-NDthREjJKFlkahMBt_kmhh_g8w9O46Dza28iaATML7stoA0-fxccmMlBvv6-T4yfdymupkEn2uSG4HW8y9e-C-MWnNUuX4Fz-Cx70muH8Px4nmWfN-tPq6vi5v3b69XlTWEEF2OhG0JLkWZqhCCl6IVgwKu2lZqC6BreNa1mTVPXwNpaStO3rairXpOuKU3bd_wsuzjo7qd2gM6AH6N2ah_tkPpSQVv194u3W_Ul3ComS044SwLnR4EYvk2AoxosmjSC9hAmVLUQtGacyoSkB6SJATFC_1CFEjX7onYq-aJmXxQpU8ycl3-298D4ZUQCvDoCNBrt-qi9sfgbx1lFGS8T7s0BB-kzby1EhcaCN9DZCGZUXbD_bePiH7Zx1ttU8CvcAe7CFH1ySVGFTBH1cV6geX9oWp2SV5TfA6XywV4</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Letourneau, Phillip A., M.D</creator><creator>Pati, Shibani, M.D., Ph.D</creator><creator>Gerber, Michael H., B.S</creator><creator>Jimenez, Fernando, M.S</creator><creator>Holcomb, John B., M.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20101001</creationdate><title>Fresh Frozen Plasma Increases Adhesion Molecule Expression on Human Pulmonary Endothelial Cells</title><author>Letourneau, Phillip A., M.D ; Pati, Shibani, M.D., Ph.D ; Gerber, Michael H., B.S ; Jimenez, Fernando, M.S ; Holcomb, John B., M.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-a80145016855045f552e36bb9a1e5d83d8ba28877e2b799cfbb576fa0d84cbfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cells, Cultured</topic><topic>E-Selectin - analysis</topic><topic>Endothelial Cells - chemistry</topic><topic>endothelium</topic><topic>General aspects</topic><topic>Humans</topic><topic>Intercellular Adhesion Molecule-1 - analysis</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lung - chemistry</topic><topic>Medical sciences</topic><topic>P-Selectin - analysis</topic><topic>plasma</topic><topic>Plasma - physiology</topic><topic>Respiratory Distress Syndrome, Adult - etiology</topic><topic>resuscitation</topic><topic>Surgery</topic><topic>trauma</topic><topic>Vascular Cell Adhesion Molecule-1 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Letourneau, Phillip A., M.D</creatorcontrib><creatorcontrib>Pati, Shibani, M.D., Ph.D</creatorcontrib><creatorcontrib>Gerber, Michael H., B.S</creatorcontrib><creatorcontrib>Jimenez, Fernando, M.S</creatorcontrib><creatorcontrib>Holcomb, John B., M.D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Letourneau, Phillip A., M.D</au><au>Pati, Shibani, M.D., Ph.D</au><au>Gerber, Michael H., B.S</au><au>Jimenez, Fernando, M.S</au><au>Holcomb, John B., M.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fresh Frozen Plasma Increases Adhesion Molecule Expression on Human Pulmonary Endothelial Cells</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>163</volume><issue>2</issue><spage>317</spage><epage>322</epage><pages>317-322</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><abstract>Background Current blood banking practices allow fresh frozen plasma (FFP) to be thawed and then stored for 5 d between 1 and 6 °C. We hypothesized that aged plasma (d 5 FFP) would be pro-inflammatory to the endothelium compared with fresh plasma (d 0 FFP). Materials and Methods Human pulmonary endothelial cells (PECs) were treated with (1) media, (2) media + lipopolysaccharide (LPS), (3) lactated Ringer's (LR), (4) LR + LPS, (5) d 0 FFP, (6) d 0 FFP + LPS, (7) d 5 FFP, and (8) d 5 FFP + LPS. After a 24 h incubation, the PECs were stained with antibodies for I-CAM, V-CAM, P-selectin, and E-selectin. The cells were subsequently analyzed by flow cytometry. Results In both PEC groups treated with FFP and stimulated with LPS, I-CAM, V-CAM, P-selectin, and E-selectin were significantly up-regulated compared with LR when stimulated by LPS. Conclusion FFP at both ages significantly increased expression of four different adhesion molecules compared with LR in PECs. This may represent a possible mechanism for increased leukocyte binding on the endothelium as a result of FFP transfusion.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>20599210</pmid><doi>10.1016/j.jss.2010.04.049</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-4804 |
| ispartof | The Journal of surgical research, 2010-10, Vol.163 (2), p.317-322 |
| issn | 0022-4804 1095-8673 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2943032 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Biological and medical sciences Cell Adhesion Molecules - analysis Cells, Cultured E-Selectin - analysis Endothelial Cells - chemistry endothelium General aspects Humans Intercellular Adhesion Molecule-1 - analysis Lipopolysaccharides - pharmacology Lung - chemistry Medical sciences P-Selectin - analysis plasma Plasma - physiology Respiratory Distress Syndrome, Adult - etiology resuscitation Surgery trauma Vascular Cell Adhesion Molecule-1 - analysis |
| title | Fresh Frozen Plasma Increases Adhesion Molecule Expression on Human Pulmonary Endothelial Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-11T14%3A12%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fresh%20Frozen%20Plasma%20Increases%20Adhesion%20Molecule%20Expression%20on%20Human%20Pulmonary%20Endothelial%20Cells&rft.jtitle=The%20Journal%20of%20surgical%20research&rft.au=Letourneau,%20Phillip%20A.,%20M.D&rft.date=2010-10-01&rft.volume=163&rft.issue=2&rft.spage=317&rft.epage=322&rft.pages=317-322&rft.issn=0022-4804&rft.eissn=1095-8673&rft.coden=JSGRA2&rft_id=info:doi/10.1016/j.jss.2010.04.049&rft_dat=%3Cproquest_pubme%3E755172319%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=755172319&rft_id=info:pmid/20599210&rft_els_id=1_s2_0_S0022480410004361&rfr_iscdi=true |