HOXA9 regulates miR-155 in hematopoietic cells
HOXA9-mediated up-regulation of miR-155 was noted during an array-based analysis of microRNA expression in Hoxa9⁻/⁻bone marrow (BM) cells. HOXA9 induction of miR-155 was confirmed in these samples, as well as in wild-type versus Hoxa9-deficient marrow, using northern analysis and qRT-PCR. Infection...
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| Veröffentlicht in: | Nucleic acids research 2010-09, Vol.38 (16), p.5472-5478 |
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| creator | Hu, Yu-Long Fong, Stephen Largman, Corey Shen, Wei-Fang |
| description | HOXA9-mediated up-regulation of miR-155 was noted during an array-based analysis of microRNA expression in Hoxa9⁻/⁻bone marrow (BM) cells. HOXA9 induction of miR-155 was confirmed in these samples, as well as in wild-type versus Hoxa9-deficient marrow, using northern analysis and qRT-PCR. Infection of wild-type BM with HOXA9 expressing or GFP⁺ control virus further confirmed HOXA9-mediated regulation of miR-155. miR-155 expression paralleled Hoxa9 mRNA expression in fractionated BM progenitors, being highest in the stem cell enriched pools. HOXA9 capacity to induce myeloid colony formation was blunted in miR-155-deficient BM cells, indicating that miR-155 is a downstream mediator of HOXA9 function in blood cells. Pu.1, an important regulator of myelopoiesis, was identified as a putative down stream target for miR-155. Although miR-155 was shown to down-regulate the Pu.1 protein, HOXA9 did not appear to modulate Pu.1 expression in murine BM cells. |
| doi_str_mv | 10.1093/nar/gkq337 |
| format | Article |
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HOXA9 induction of miR-155 was confirmed in these samples, as well as in wild-type versus Hoxa9-deficient marrow, using northern analysis and qRT-PCR. Infection of wild-type BM with HOXA9 expressing or GFP⁺ control virus further confirmed HOXA9-mediated regulation of miR-155. miR-155 expression paralleled Hoxa9 mRNA expression in fractionated BM progenitors, being highest in the stem cell enriched pools. HOXA9 capacity to induce myeloid colony formation was blunted in miR-155-deficient BM cells, indicating that miR-155 is a downstream mediator of HOXA9 function in blood cells. Pu.1, an important regulator of myelopoiesis, was identified as a putative down stream target for miR-155. Although miR-155 was shown to down-regulate the Pu.1 protein, HOXA9 did not appear to modulate Pu.1 expression in murine BM cells.</description><identifier>ISSN: 0305-1048</identifier><identifier>ISSN: 1362-4962</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/gkq337</identifier><identifier>PMID: 20444872</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Cells, Cultured ; Gene Expression Regulation ; Hematopoietic Stem Cells - metabolism ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Mice ; MicroRNAs - biosynthesis ; MicroRNAs - metabolism ; Molecular Biology ; Proto-Oncogene Proteins - metabolism ; Trans-Activators - metabolism ; Up-Regulation</subject><ispartof>Nucleic acids research, 2010-09, Vol.38 (16), p.5472-5478</ispartof><rights>The Author(s) 2010. 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HOXA9 induction of miR-155 was confirmed in these samples, as well as in wild-type versus Hoxa9-deficient marrow, using northern analysis and qRT-PCR. Infection of wild-type BM with HOXA9 expressing or GFP⁺ control virus further confirmed HOXA9-mediated regulation of miR-155. miR-155 expression paralleled Hoxa9 mRNA expression in fractionated BM progenitors, being highest in the stem cell enriched pools. HOXA9 capacity to induce myeloid colony formation was blunted in miR-155-deficient BM cells, indicating that miR-155 is a downstream mediator of HOXA9 function in blood cells. Pu.1, an important regulator of myelopoiesis, was identified as a putative down stream target for miR-155. Although miR-155 was shown to down-regulate the Pu.1 protein, HOXA9 did not appear to modulate Pu.1 expression in murine BM cells.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Gene Expression Regulation</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Mice</subject><subject>MicroRNAs - biosynthesis</subject><subject>MicroRNAs - metabolism</subject><subject>Molecular Biology</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Trans-Activators - metabolism</subject><subject>Up-Regulation</subject><issn>0305-1048</issn><issn>1362-4962</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0ctKAzEUBuAgitbqxgfQ2QnC6Dm5ZyOIqBUEwQu4C-k000bnUpOp4Ns7Ui260tWBnI-fHH5C9hCOEQw7aVw8mb68MqbWyACZpDk3kq6TATAQOQLXW2Q7pWcA5Cj4JtmiwDnXig7I8ej26cxk0U8Xlet8yupwl6MQWWiyma9d187b4LtQZIWvqrRDNkpXJb_7NYfk8fLi4XyU39xeXZ-f3eQFl6LLvSkAhPLMO8XNWKGZoGNKTgQ1jk-k8sBlqZBxNHIsmKNaecNKwRBp0b8Pyekyd74Y135S-KaLrrLzGGoX323rgv29acLMTts3Sw3TFGkfcPgVENvXhU-drUP6PME1vl0kqySlGoHJf0lpeG__lIIDaBC6l0dLWcQ2pejL1c8R7Gdntu_MLjvr8f7PW1f0u6QeHCxB6VrrpjEk-3hPARmg1oorzT4Ak0eZTA</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Hu, Yu-Long</creator><creator>Fong, Stephen</creator><creator>Largman, Corey</creator><creator>Shen, Wei-Fang</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20100901</creationdate><title>HOXA9 regulates miR-155 in hematopoietic cells</title><author>Hu, Yu-Long ; Fong, Stephen ; Largman, Corey ; Shen, Wei-Fang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-e9c0057e3ea749b719d1a376d529a4d67e046f7134196b53a287e93f53112c713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Gene Expression Regulation</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Mice</topic><topic>MicroRNAs - biosynthesis</topic><topic>MicroRNAs - metabolism</topic><topic>Molecular Biology</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Trans-Activators - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Yu-Long</creatorcontrib><creatorcontrib>Fong, Stephen</creatorcontrib><creatorcontrib>Largman, Corey</creatorcontrib><creatorcontrib>Shen, Wei-Fang</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Yu-Long</au><au>Fong, Stephen</au><au>Largman, Corey</au><au>Shen, Wei-Fang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HOXA9 regulates miR-155 in hematopoietic cells</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2010-09-01</date><risdate>2010</risdate><volume>38</volume><issue>16</issue><spage>5472</spage><epage>5478</epage><pages>5472-5478</pages><issn>0305-1048</issn><issn>1362-4962</issn><eissn>1362-4962</eissn><abstract>HOXA9-mediated up-regulation of miR-155 was noted during an array-based analysis of microRNA expression in Hoxa9⁻/⁻bone marrow (BM) cells. HOXA9 induction of miR-155 was confirmed in these samples, as well as in wild-type versus Hoxa9-deficient marrow, using northern analysis and qRT-PCR. Infection of wild-type BM with HOXA9 expressing or GFP⁺ control virus further confirmed HOXA9-mediated regulation of miR-155. miR-155 expression paralleled Hoxa9 mRNA expression in fractionated BM progenitors, being highest in the stem cell enriched pools. HOXA9 capacity to induce myeloid colony formation was blunted in miR-155-deficient BM cells, indicating that miR-155 is a downstream mediator of HOXA9 function in blood cells. Pu.1, an important regulator of myelopoiesis, was identified as a putative down stream target for miR-155. Although miR-155 was shown to down-regulate the Pu.1 protein, HOXA9 did not appear to modulate Pu.1 expression in murine BM cells.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>20444872</pmid><doi>10.1093/nar/gkq337</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford Journals Open Access Collection; MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Cells, Cultured Gene Expression Regulation Hematopoietic Stem Cells - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Mice MicroRNAs - biosynthesis MicroRNAs - metabolism Molecular Biology Proto-Oncogene Proteins - metabolism Trans-Activators - metabolism Up-Regulation |
| title | HOXA9 regulates miR-155 in hematopoietic cells |
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