Alteration of the phospho- or neutral lipid content and fatty acid composition in Listeria monocytogenes due to acid adaptation mechanisms for hydrochloric, acetic and lactic acids at pH 5.5 or benzoic acid at neutral pH
This study provides a first approach to observe the effects on Listeria monocytogenes of cellular exposure to acid stress at low or neutral pH, notably how phospho- or neutral lipids are involved in this mechanism, besides the fatty acid profile alteration. A thorough investigation of the compositio...
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2010-10, Vol.98 (3), p.307-316 |
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| creator | Mastronicolis, Sofia K Berberi, Anita Diakogiannis, Ioannis Petrova, Evanthia Kiaki, Irene Baltzi, Triantafillia Xenikakis, Polydoros |
| description | This study provides a first approach to observe the effects on Listeria monocytogenes of cellular exposure to acid stress at low or neutral pH, notably how phospho- or neutral lipids are involved in this mechanism, besides the fatty acid profile alteration. A thorough investigation of the composition of polar and neutral lipids from L. monocytogenes grown at pH 5.5 in presence of hydrochloric, acetic and lactic acids, or at neutral pH 7.3 in presence of benzoic acid, is described relative to cells grown in acid-free medium. The results showed that only low pH values enhance the antimicrobial activity of an acid. We suggest that, irrespective of pH, the acid adaptation response will lead to a similar alteration in fatty acid composition [decreasing the ratio of branched chain/saturated straight fatty acids of total lipids], mainly originating from the neutral lipid class of adapted cultures. Acid adaptation in L. monocytogenes was correlated with a decrease in total lipid phosphorus and, with the exception of cells adapted to benzoic acid, this change in the amount of phosphorus reflected a higher content of the neutral lipid class. Upon acetic or benzoic acid stress the lipid phosphorus proportion was analysed in the main phospholipids present: cardiolipin, phosphatidylglycerol, phosphoaminolipid and phosphatidylinositol. Interestingly only benzoic acid had a dramatic effect on the relative quantities of these four phospholipids. |
| doi_str_mv | 10.1007/s10482-010-9439-z |
| format | Article |
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A thorough investigation of the composition of polar and neutral lipids from L. monocytogenes grown at pH 5.5 in presence of hydrochloric, acetic and lactic acids, or at neutral pH 7.3 in presence of benzoic acid, is described relative to cells grown in acid-free medium. The results showed that only low pH values enhance the antimicrobial activity of an acid. We suggest that, irrespective of pH, the acid adaptation response will lead to a similar alteration in fatty acid composition [decreasing the ratio of branched chain/saturated straight fatty acids of total lipids], mainly originating from the neutral lipid class of adapted cultures. Acid adaptation in L. monocytogenes was correlated with a decrease in total lipid phosphorus and, with the exception of cells adapted to benzoic acid, this change in the amount of phosphorus reflected a higher content of the neutral lipid class. Upon acetic or benzoic acid stress the lipid phosphorus proportion was analysed in the main phospholipids present: cardiolipin, phosphatidylglycerol, phosphoaminolipid and phosphatidylinositol. Interestingly only benzoic acid had a dramatic effect on the relative quantities of these four phospholipids.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-010-9439-z</identifier><identifier>PMID: 20379849</identifier><identifier>CODEN: ANLEDR</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Acetic Acid - metabolism ; Acid adaptation ; Adaptation, Physiological ; Adaptations ; Antimicrobial activity ; Bacteriology ; Benzoic acid ; Benzoic Acid - metabolism ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; cardiolipin ; Cardiolipins - metabolism ; Cell culture ; Fatty acid composition ; Fatty acids ; Fatty Acids - metabolism ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; hydrochloric acid ; Hydrochloric Acid - metabolism ; Hydrogen-Ion Concentration ; Lactic acid ; Lactic Acid - metabolism ; Life Sciences ; Lipids ; Listeria monocytogenes ; Listeria monocytogenes - chemistry ; Listeria monocytogenes - growth & development ; Listeria monocytogenes - metabolism ; Medical Microbiology ; Membrane Lipids - analysis ; Membrane Lipids - metabolism ; Microbiology ; Miscellaneous ; Neutral lipids and phospholipids ; Original Paper ; pH effects ; phosphatidylglycerol ; Phosphatidylglycerols - metabolism ; phosphatidylinositol ; Phosphatidylinositols - metabolism ; Phospholipids ; Phospholipids - metabolism ; Phosphorus ; Plant Sciences ; Soil Science & Conservation ; Stress ; Stress, Physiological ; Weak acid food preservative</subject><ispartof>Antonie van Leeuwenhoek, 2010-10, Vol.98 (3), p.307-316</ispartof><rights>The Author(s) 2010</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c620t-4b1b1b310187352092e470f9c1c8a03304afb88e53dcc657cffcc7d93bb660393</citedby><cites>FETCH-LOGICAL-c620t-4b1b1b310187352092e470f9c1c8a03304afb88e53dcc657cffcc7d93bb660393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-010-9439-z$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-010-9439-z$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23222992$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20379849$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mastronicolis, Sofia K</creatorcontrib><creatorcontrib>Berberi, Anita</creatorcontrib><creatorcontrib>Diakogiannis, Ioannis</creatorcontrib><creatorcontrib>Petrova, Evanthia</creatorcontrib><creatorcontrib>Kiaki, Irene</creatorcontrib><creatorcontrib>Baltzi, Triantafillia</creatorcontrib><creatorcontrib>Xenikakis, Polydoros</creatorcontrib><title>Alteration of the phospho- or neutral lipid content and fatty acid composition in Listeria monocytogenes due to acid adaptation mechanisms for hydrochloric, acetic and lactic acids at pH 5.5 or benzoic acid at neutral pH</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>This study provides a first approach to observe the effects on Listeria monocytogenes of cellular exposure to acid stress at low or neutral pH, notably how phospho- or neutral lipids are involved in this mechanism, besides the fatty acid profile alteration. A thorough investigation of the composition of polar and neutral lipids from L. monocytogenes grown at pH 5.5 in presence of hydrochloric, acetic and lactic acids, or at neutral pH 7.3 in presence of benzoic acid, is described relative to cells grown in acid-free medium. The results showed that only low pH values enhance the antimicrobial activity of an acid. We suggest that, irrespective of pH, the acid adaptation response will lead to a similar alteration in fatty acid composition [decreasing the ratio of branched chain/saturated straight fatty acids of total lipids], mainly originating from the neutral lipid class of adapted cultures. Acid adaptation in L. monocytogenes was correlated with a decrease in total lipid phosphorus and, with the exception of cells adapted to benzoic acid, this change in the amount of phosphorus reflected a higher content of the neutral lipid class. Upon acetic or benzoic acid stress the lipid phosphorus proportion was analysed in the main phospholipids present: cardiolipin, phosphatidylglycerol, phosphoaminolipid and phosphatidylinositol. Interestingly only benzoic acid had a dramatic effect on the relative quantities of these four phospholipids.</description><subject>Acetic Acid - metabolism</subject><subject>Acid adaptation</subject><subject>Adaptation, Physiological</subject><subject>Adaptations</subject><subject>Antimicrobial activity</subject><subject>Bacteriology</subject><subject>Benzoic acid</subject><subject>Benzoic Acid - metabolism</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>cardiolipin</subject><subject>Cardiolipins - metabolism</subject><subject>Cell culture</subject><subject>Fatty acid composition</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hydrochloric acid</subject><subject>Hydrochloric Acid - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lactic acid</subject><subject>Lactic Acid - metabolism</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - chemistry</subject><subject>Listeria monocytogenes - growth & development</subject><subject>Listeria monocytogenes - metabolism</subject><subject>Medical Microbiology</subject><subject>Membrane Lipids - analysis</subject><subject>Membrane Lipids - metabolism</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Neutral lipids and phospholipids</subject><subject>Original Paper</subject><subject>pH effects</subject><subject>phosphatidylglycerol</subject><subject>Phosphatidylglycerols - metabolism</subject><subject>phosphatidylinositol</subject><subject>Phosphatidylinositols - metabolism</subject><subject>Phospholipids</subject><subject>Phospholipids - metabolism</subject><subject>Phosphorus</subject><subject>Plant Sciences</subject><subject>Soil Science & Conservation</subject><subject>Stress</subject><subject>Stress, Physiological</subject><subject>Weak acid food preservative</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9Us1u1DAYjBCILoUH4AIWEuJCin-S2L4gVRWwSCtxgJ4tx7E3rhI72E6l3WflYfButi1wQFEUx9_MfPPZUxQvEbxAENIPEcGK4RIiWPKK8HL_qFihmuKSN5w_LlYQQlI2kOKz4lmMN_mXN4w-Lc4wJJSziq-KX5dD0kEm6x3wBqReg6n3Mb8l8AE4PacgBzDYyXZAeZe0S0C6DhiZ0g5IddweJx_tUcM6sLExS1oJRu-82iW_1U5H0M0aJL8wZCentDQdteqls3GMwOSG_a4LXvWDD1a9z2CdrDr2G6Q6LjM9ApnAtAb1RX3w2Gq396fSoXLneVo_L54YOUT94vQ9L64_f_pxtS433758vbrclKrBMJVVi_JDEESMkhpDjnVFoeEKKSYhIbCSpmVM16RTqqmpMkYp2nHStk0DCSfnxcdFd5rbUXcqn1E2IKZgRxl2wksr_q4424utvxWYk5pTnAXenQSC_znrmMRoo9LDIJ32cxQs32HDCawz8s0_yBs_B5enE7SGjFcNazIILSAVfIxBm3srCIpDcsSSHJGTIw7JEfvMefXnDPeMu6hkwNsTQEYlBxOkUzY-4AjGmPPDLHjBxVxyWx0eHP6v--uFZKQXchuy8PV3DBHJd8LyYIT8BjrZ6eg</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Mastronicolis, Sofia K</creator><creator>Berberi, Anita</creator><creator>Diakogiannis, Ioannis</creator><creator>Petrova, Evanthia</creator><creator>Kiaki, Irene</creator><creator>Baltzi, Triantafillia</creator><creator>Xenikakis, Polydoros</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20101001</creationdate><title>Alteration of the phospho- or neutral lipid content and fatty acid composition in Listeria monocytogenes due to acid adaptation mechanisms for hydrochloric, acetic and lactic acids at pH 5.5 or benzoic acid at neutral pH</title><author>Mastronicolis, Sofia K ; Berberi, Anita ; Diakogiannis, Ioannis ; Petrova, Evanthia ; Kiaki, Irene ; Baltzi, Triantafillia ; Xenikakis, Polydoros</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c620t-4b1b1b310187352092e470f9c1c8a03304afb88e53dcc657cffcc7d93bb660393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetic Acid - metabolism</topic><topic>Acid adaptation</topic><topic>Adaptation, Physiological</topic><topic>Adaptations</topic><topic>Antimicrobial activity</topic><topic>Bacteriology</topic><topic>Benzoic acid</topic><topic>Benzoic Acid - metabolism</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>cardiolipin</topic><topic>Cardiolipins - metabolism</topic><topic>Cell culture</topic><topic>Fatty acid composition</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hydrochloric acid</topic><topic>Hydrochloric Acid - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lactic acid</topic><topic>Lactic Acid - metabolism</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - chemistry</topic><topic>Listeria monocytogenes - growth & development</topic><topic>Listeria monocytogenes - metabolism</topic><topic>Medical Microbiology</topic><topic>Membrane Lipids - analysis</topic><topic>Membrane Lipids - metabolism</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Neutral lipids and phospholipids</topic><topic>Original Paper</topic><topic>pH effects</topic><topic>phosphatidylglycerol</topic><topic>Phosphatidylglycerols - metabolism</topic><topic>phosphatidylinositol</topic><topic>Phosphatidylinositols - metabolism</topic><topic>Phospholipids</topic><topic>Phospholipids - metabolism</topic><topic>Phosphorus</topic><topic>Plant 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Polydoros</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alteration of the phospho- or neutral lipid content and fatty acid composition in Listeria monocytogenes due to acid adaptation mechanisms for hydrochloric, acetic and lactic acids at pH 5.5 or benzoic acid at neutral pH</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>98</volume><issue>3</issue><spage>307</spage><epage>316</epage><pages>307-316</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><coden>ANLEDR</coden><abstract>This study provides a first approach to observe the effects on Listeria monocytogenes of cellular exposure to acid stress at low or neutral pH, notably how phospho- or neutral lipids are involved in this mechanism, besides the fatty acid profile alteration. A thorough investigation of the composition of polar and neutral lipids from L. monocytogenes grown at pH 5.5 in presence of hydrochloric, acetic and lactic acids, or at neutral pH 7.3 in presence of benzoic acid, is described relative to cells grown in acid-free medium. The results showed that only low pH values enhance the antimicrobial activity of an acid. We suggest that, irrespective of pH, the acid adaptation response will lead to a similar alteration in fatty acid composition [decreasing the ratio of branched chain/saturated straight fatty acids of total lipids], mainly originating from the neutral lipid class of adapted cultures. Acid adaptation in L. monocytogenes was correlated with a decrease in total lipid phosphorus and, with the exception of cells adapted to benzoic acid, this change in the amount of phosphorus reflected a higher content of the neutral lipid class. Upon acetic or benzoic acid stress the lipid phosphorus proportion was analysed in the main phospholipids present: cardiolipin, phosphatidylglycerol, phosphoaminolipid and phosphatidylinositol. Interestingly only benzoic acid had a dramatic effect on the relative quantities of these four phospholipids.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>20379849</pmid><doi>10.1007/s10482-010-9439-z</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Antonie van Leeuwenhoek, 2010-10, Vol.98 (3), p.307-316 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Acetic Acid - metabolism Acid adaptation Adaptation, Physiological Adaptations Antimicrobial activity Bacteriology Benzoic acid Benzoic Acid - metabolism Biochemistry Biological and medical sciences Biomedical and Life Sciences cardiolipin Cardiolipins - metabolism Cell culture Fatty acid composition Fatty acids Fatty Acids - metabolism Food Microbiology Fundamental and applied biological sciences. Psychology hydrochloric acid Hydrochloric Acid - metabolism Hydrogen-Ion Concentration Lactic acid Lactic Acid - metabolism Life Sciences Lipids Listeria monocytogenes Listeria monocytogenes - chemistry Listeria monocytogenes - growth & development Listeria monocytogenes - metabolism Medical Microbiology Membrane Lipids - analysis Membrane Lipids - metabolism Microbiology Miscellaneous Neutral lipids and phospholipids Original Paper pH effects phosphatidylglycerol Phosphatidylglycerols - metabolism phosphatidylinositol Phosphatidylinositols - metabolism Phospholipids Phospholipids - metabolism Phosphorus Plant Sciences Soil Science & Conservation Stress Stress, Physiological Weak acid food preservative |
| title | Alteration of the phospho- or neutral lipid content and fatty acid composition in Listeria monocytogenes due to acid adaptation mechanisms for hydrochloric, acetic and lactic acids at pH 5.5 or benzoic acid at neutral pH |
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