Integration of a Precolumn Fluorogenic Reaction, Separation, and Detection of Reduced Glutathione

Reduced glutathione (GSH) has been determined by fluorescence detection after derivatization together with a variety of separations. The reactions between GSH and fluorescent reagents usually are carried out during the sample pretreatment and require minutes to hours for complete reactions. For cont...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-09, Vol.82 (17), p.7267-7273
Hauptverfasser:	Wu, Juanfang, Ferrance, Jerome P, Landers, James P, Weber, Stephen G
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemical reactions Chemistry Enzyme kinetics Exact sciences and technology Fluorescence Fluorescent Dyes - chemistry Glutathione - analysis Glutathione - chemistry Glutathione - isolation & purification Hydrogen-Ion Concentration Kinetics Membrane separation Microfluidic Analytical Techniques NADP - chemistry Naphthalenes - chemistry Oxidation-Reduction Proteins Pyrroles - chemistry Reaction kinetics Spectrometric and optical methods Spectrometry, Fluorescence - methods Temperature
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