Mucin gene 19 (MUC19) expression and response to inflammatory cytokines in middle ear epithelium
Mucin gene 19 (MUC19) has been identified as a major gel-forming mucin in the human middle ear (ME). The objectives of this investigation were to characterize the expression and assess the regulation of MUC19 in the ME cell culture models utilized in the study of otitis media (OM). Findings demonstr...
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| Veröffentlicht in: | Glycoconjugate journal 2009-12, Vol.26 (9), p.1275-1284 |
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| creator | Kerschner, Joseph E Khampang, Pawjai Erbe, Christy B Kolker, Alexander Cioffi, Joseph A |
| description | Mucin gene 19 (MUC19) has been identified as a major gel-forming mucin in the human middle ear (ME). The objectives of this investigation were to characterize the expression and assess the regulation of MUC19 in the ME cell culture models utilized in the study of otitis media (OM). Findings demonstrate that MUC19 is expressed in both human immortalized cell culture (HMEEC) and chinchilla primary epithelial culture (CMEEC). ME exposure to inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8 up-regulate MUC19 transcription, most robustly after exposure to TNF-α. Kinetic experiments suggest a relative early response in MUC19 transcription and a down-regulation after prolonged exposure. Glycoprotein production was increased in response to the increased transcription as well. Similar to other mucin genes in the ME, MUC19 is differentially regulated after exposure to inflammatory cytokines. The large size, gel-forming properties and up-regulation in response to important inflammatory cytokines of MUC19 suggest that it has significant potential to play a role in both physiology and pathophysiology of the ME. |
| doi_str_mv | 10.1007/s10719-009-9245-x |
| format | Article |
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The objectives of this investigation were to characterize the expression and assess the regulation of MUC19 in the ME cell culture models utilized in the study of otitis media (OM). Findings demonstrate that MUC19 is expressed in both human immortalized cell culture (HMEEC) and chinchilla primary epithelial culture (CMEEC). ME exposure to inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8 up-regulate MUC19 transcription, most robustly after exposure to TNF-α. Kinetic experiments suggest a relative early response in MUC19 transcription and a down-regulation after prolonged exposure. Glycoprotein production was increased in response to the increased transcription as well. Similar to other mucin genes in the ME, MUC19 is differentially regulated after exposure to inflammatory cytokines. The large size, gel-forming properties and up-regulation in response to important inflammatory cytokines of MUC19 suggest that it has significant potential to play a role in both physiology and pathophysiology of the ME.</description><identifier>ISSN: 0282-0080</identifier><identifier>EISSN: 1573-4986</identifier><identifier>DOI: 10.1007/s10719-009-9245-x</identifier><identifier>PMID: 19533339</identifier><language>eng</language><publisher>Boston: Boston : Springer US</publisher><subject>Animals ; Biochemistry ; Biomedical and Life Sciences ; Cell culture ; Chinchilla ; Cytokines - pharmacology ; Ear diseases ; Ear, Middle - metabolism ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Epithelium - drug effects ; Epithelium - metabolism ; Gene expression ; Gene Expression Regulation - drug effects ; Humans ; Inflammation Mediators - pharmacology ; Inflammatory diseases ; Interleukin-1beta - pharmacology ; Interleukin-6 - pharmacology ; Interleukin-8 - pharmacology ; Life Sciences ; Mucins - genetics ; Mucins - metabolism ; Pathology ; Polymerase Chain Reaction ; Rodentia ; Time Factors ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Glycoconjugate journal, 2009-12, Vol.26 (9), p.1275-1284</ispartof><rights>Springer Science+Business Media, LLC 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-dc98360006942d0699f0bd56a58479ab551443f94e2f407d8aa85a7133975513</citedby><cites>FETCH-LOGICAL-c523t-dc98360006942d0699f0bd56a58479ab551443f94e2f407d8aa85a7133975513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10719-009-9245-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10719-009-9245-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19533339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kerschner, Joseph E</creatorcontrib><creatorcontrib>Khampang, Pawjai</creatorcontrib><creatorcontrib>Erbe, Christy B</creatorcontrib><creatorcontrib>Kolker, Alexander</creatorcontrib><creatorcontrib>Cioffi, Joseph A</creatorcontrib><title>Mucin gene 19 (MUC19) expression and response to inflammatory cytokines in middle ear epithelium</title><title>Glycoconjugate journal</title><addtitle>Glycoconj J</addtitle><addtitle>Glycoconj J</addtitle><description>Mucin gene 19 (MUC19) has been identified as a major gel-forming mucin in the human middle ear (ME). The objectives of this investigation were to characterize the expression and assess the regulation of MUC19 in the ME cell culture models utilized in the study of otitis media (OM). Findings demonstrate that MUC19 is expressed in both human immortalized cell culture (HMEEC) and chinchilla primary epithelial culture (CMEEC). ME exposure to inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8 up-regulate MUC19 transcription, most robustly after exposure to TNF-α. Kinetic experiments suggest a relative early response in MUC19 transcription and a down-regulation after prolonged exposure. Glycoprotein production was increased in response to the increased transcription as well. Similar to other mucin genes in the ME, MUC19 is differentially regulated after exposure to inflammatory cytokines. The large size, gel-forming properties and up-regulation in response to important inflammatory cytokines of MUC19 suggest that it has significant potential to play a role in both physiology and pathophysiology of the ME.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell culture</subject><subject>Chinchilla</subject><subject>Cytokines - pharmacology</subject><subject>Ear diseases</subject><subject>Ear, Middle - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Inflammation Mediators - pharmacology</subject><subject>Inflammatory diseases</subject><subject>Interleukin-1beta - pharmacology</subject><subject>Interleukin-6 - pharmacology</subject><subject>Interleukin-8 - pharmacology</subject><subject>Life Sciences</subject><subject>Mucins - genetics</subject><subject>Mucins - metabolism</subject><subject>Pathology</subject><subject>Polymerase Chain Reaction</subject><subject>Rodentia</subject><subject>Time Factors</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0282-0080</issn><issn>1573-4986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9Uk1vFCEYJkZjt6s_wIsSD40eRvkc4GJiNn4lbTzYnpGdeWdLnYEpzJjdfy_NbLR6EBIgPB-8bx4QekbJG0qIepspUdRUhJjKMCGr_QO0olLxShhdP0QrwjQrqCYn6DTnG1I0gunH6IQaycswK_T9Ym58wDsIgKnBry6uNtS8xrAfE-TsY8AutLicxxgy4CliH7reDYObYjrg5jDFHz5ALtd48G3bAwaXMIx-uobez8MT9KhzfYanx32NLj9-uNx8rs6_fvqyeX9eNZLxqWobo3ldKqyNYG1ZTUe2rayd1EIZt5WSCsE7I4B1gqhWO6elU7Q0oQrG1-jdYjvO2wHaBsKUXG_H5AeXDjY6b_9Ggr-2u_jTMkM143UxODsapHg7Q57s4HMDfe8CxDlbRpnUjOpCfPkP8SbOKZTeLDWKay7KXCO6kJoUc07Q_a6EEnuXnV2ysyU7e5ed3RfN8_st_FEcwyoEthBygcIO0r2X_-P6YhF1Llq3Sz7bq2-MUE6oKn-F1vwXkTKtXg</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Kerschner, Joseph E</creator><creator>Khampang, Pawjai</creator><creator>Erbe, Christy B</creator><creator>Kolker, Alexander</creator><creator>Cioffi, Joseph A</creator><general>Boston : Springer US</general><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20091201</creationdate><title>Mucin gene 19 (MUC19) expression and response to inflammatory cytokines in middle ear epithelium</title><author>Kerschner, Joseph E ; Khampang, Pawjai ; Erbe, Christy B ; Kolker, Alexander ; Cioffi, Joseph A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-dc98360006942d0699f0bd56a58479ab551443f94e2f407d8aa85a7133975513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell culture</topic><topic>Chinchilla</topic><topic>Cytokines - pharmacology</topic><topic>Ear diseases</topic><topic>Ear, Middle - metabolism</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Inflammation Mediators - pharmacology</topic><topic>Inflammatory diseases</topic><topic>Interleukin-1beta - pharmacology</topic><topic>Interleukin-6 - pharmacology</topic><topic>Interleukin-8 - pharmacology</topic><topic>Life Sciences</topic><topic>Mucins - genetics</topic><topic>Mucins - metabolism</topic><topic>Pathology</topic><topic>Polymerase Chain Reaction</topic><topic>Rodentia</topic><topic>Time Factors</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kerschner, Joseph E</creatorcontrib><creatorcontrib>Khampang, Pawjai</creatorcontrib><creatorcontrib>Erbe, Christy B</creatorcontrib><creatorcontrib>Kolker, Alexander</creatorcontrib><creatorcontrib>Cioffi, Joseph A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Glycoconjugate journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kerschner, Joseph E</au><au>Khampang, Pawjai</au><au>Erbe, Christy B</au><au>Kolker, Alexander</au><au>Cioffi, Joseph A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mucin gene 19 (MUC19) expression and response to inflammatory cytokines in middle ear epithelium</atitle><jtitle>Glycoconjugate journal</jtitle><stitle>Glycoconj J</stitle><addtitle>Glycoconj J</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>26</volume><issue>9</issue><spage>1275</spage><epage>1284</epage><pages>1275-1284</pages><issn>0282-0080</issn><eissn>1573-4986</eissn><abstract>Mucin gene 19 (MUC19) has been identified as a major gel-forming mucin in the human middle ear (ME). The objectives of this investigation were to characterize the expression and assess the regulation of MUC19 in the ME cell culture models utilized in the study of otitis media (OM). Findings demonstrate that MUC19 is expressed in both human immortalized cell culture (HMEEC) and chinchilla primary epithelial culture (CMEEC). ME exposure to inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8 up-regulate MUC19 transcription, most robustly after exposure to TNF-α. Kinetic experiments suggest a relative early response in MUC19 transcription and a down-regulation after prolonged exposure. Glycoprotein production was increased in response to the increased transcription as well. Similar to other mucin genes in the ME, MUC19 is differentially regulated after exposure to inflammatory cytokines. The large size, gel-forming properties and up-regulation in response to important inflammatory cytokines of MUC19 suggest that it has significant potential to play a role in both physiology and pathophysiology of the ME.</abstract><cop>Boston</cop><pub>Boston : Springer US</pub><pmid>19533339</pmid><doi>10.1007/s10719-009-9245-x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biochemistry Biomedical and Life Sciences Cell culture Chinchilla Cytokines - pharmacology Ear diseases Ear, Middle - metabolism Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelium - drug effects Epithelium - metabolism Gene expression Gene Expression Regulation - drug effects Humans Inflammation Mediators - pharmacology Inflammatory diseases Interleukin-1beta - pharmacology Interleukin-6 - pharmacology Interleukin-8 - pharmacology Life Sciences Mucins - genetics Mucins - metabolism Pathology Polymerase Chain Reaction Rodentia Time Factors Tumor Necrosis Factor-alpha - pharmacology |
| title | Mucin gene 19 (MUC19) expression and response to inflammatory cytokines in middle ear epithelium |
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