Epidemiological and Phylogenetic Analysis of Spanish Human Brucella melitensis Strains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, and rpoB Typing
The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the diffe...
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| description | The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase β subunit (rpoB) were used to type a human Brucella melitensis population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (37.2 to 93.7%). The population clustered into the following groups: American, with genotypes 47 (1 strain), 48 (13 strains), 53 (12 strains), 55 (2 strains), 80 (1 strain), and a new genotype (2 strains), Western Mediterranean, with genotype 51 (9 strains), and Eastern Mediterranean, with genotypes 42 (60 strains), 43 (4 strains), and 63 (4 strains). Two profession-related and two foodborne acquisitions were confirmed. Distributed throughout Spain, Eastern Mediterranean genotype 42 was the most common (55%). The low MLVA-16 allelic polymorphism (genetic similarity range, 75 to 94%) of the genotype 42 strains suggests that they recently evolved from a common ancestor. rpoB typing grouped the strains as rpoB type 1 (1249-ATG/1309-CTG; 28.7%), rpoB type 2 (1249-ATG/1309-CTA; 62.9%), and rpoB type 3 (1249-ATA/1309-CTG; 8.3%). According to the MLVA-16 results, the population clustered by rpoB type. Given the correlation between B. melitensis MLVA groups and rpoB types (American and rpoB type 1, Eastern Mediterranean and rpoB type 2, and Western Mediterranean and rpoB type 3), the rpoB type could be used as an initial marker for the epidemiological surveillance of brucellosis. |
| doi_str_mv | 10.1128/JCM.00533-10 |
| format | Article |
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Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase β subunit (rpoB) were used to type a human Brucella melitensis population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (37.2 to 93.7%). The population clustered into the following groups: American, with genotypes 47 (1 strain), 48 (13 strains), 53 (12 strains), 55 (2 strains), 80 (1 strain), and a new genotype (2 strains), Western Mediterranean, with genotype 51 (9 strains), and Eastern Mediterranean, with genotypes 42 (60 strains), 43 (4 strains), and 63 (4 strains). Two profession-related and two foodborne acquisitions were confirmed. Distributed throughout Spain, Eastern Mediterranean genotype 42 was the most common (55%). The low MLVA-16 allelic polymorphism (genetic similarity range, 75 to 94%) of the genotype 42 strains suggests that they recently evolved from a common ancestor. rpoB typing grouped the strains as rpoB type 1 (1249-ATG/1309-CTG; 28.7%), rpoB type 2 (1249-ATG/1309-CTA; 62.9%), and rpoB type 3 (1249-ATA/1309-CTG; 8.3%). According to the MLVA-16 results, the population clustered by rpoB type. Given the correlation between B. melitensis MLVA groups and rpoB types (American and rpoB type 1, Eastern Mediterranean and rpoB type 2, and Western Mediterranean and rpoB type 3), the rpoB type could be used as an initial marker for the epidemiological surveillance of brucellosis.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.00533-10</identifier><identifier>PMID: 20554816</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacterial Proteins - genetics ; Bacterial Typing Techniques ; Bacteriology ; Biological and medical sciences ; Brucella melitensis ; Brucella melitensis - classification ; Brucella melitensis - genetics ; Brucella melitensis - isolation & purification ; Brucellosis - epidemiology ; Brucellosis - microbiology ; Cluster Analysis ; DNA Fingerprinting ; DNA, Bacterial - genetics ; DNA-Directed RNA Polymerases - genetics ; Fundamental and applied biological sciences. Psychology ; Genotype ; Humans ; Microbiology ; Minisatellite Repeats ; Miscellaneous ; Molecular Epidemiology ; Oligonucleotides - genetics ; Polymorphism, Genetic ; Spain - epidemiology</subject><ispartof>Journal of Clinical Microbiology, 2010-08, Vol.48 (8), p.2734-2740</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010, American Society for Microbiology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-3dc66a59c9cdb35ffe7a3a3bee00a16f1d6cd91885e8e32e8fb6b1eae111b13d3</citedby><cites>FETCH-LOGICAL-c489t-3dc66a59c9cdb35ffe7a3a3bee00a16f1d6cd91885e8e32e8fb6b1eae111b13d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916618/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916618/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23083272$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20554816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valdezate, Sylvia</creatorcontrib><creatorcontrib>Navarro, Ana</creatorcontrib><creatorcontrib>Villalón, Pilar</creatorcontrib><creatorcontrib>Carrasco, Gema</creatorcontrib><creatorcontrib>Saéz-Nieto, Juan A</creatorcontrib><title>Epidemiological and Phylogenetic Analysis of Spanish Human Brucella melitensis Strains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, and rpoB Typing</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase β subunit (rpoB) were used to type a human Brucella melitensis population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (37.2 to 93.7%). The population clustered into the following groups: American, with genotypes 47 (1 strain), 48 (13 strains), 53 (12 strains), 55 (2 strains), 80 (1 strain), and a new genotype (2 strains), Western Mediterranean, with genotype 51 (9 strains), and Eastern Mediterranean, with genotypes 42 (60 strains), 43 (4 strains), and 63 (4 strains). Two profession-related and two foodborne acquisitions were confirmed. Distributed throughout Spain, Eastern Mediterranean genotype 42 was the most common (55%). The low MLVA-16 allelic polymorphism (genetic similarity range, 75 to 94%) of the genotype 42 strains suggests that they recently evolved from a common ancestor. rpoB typing grouped the strains as rpoB type 1 (1249-ATG/1309-CTG; 28.7%), rpoB type 2 (1249-ATG/1309-CTA; 62.9%), and rpoB type 3 (1249-ATA/1309-CTG; 8.3%). According to the MLVA-16 results, the population clustered by rpoB type. Given the correlation between B. melitensis MLVA groups and rpoB types (American and rpoB type 1, Eastern Mediterranean and rpoB type 2, and Western Mediterranean and rpoB type 3), the rpoB type could be used as an initial marker for the epidemiological surveillance of brucellosis.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Brucella melitensis</subject><subject>Brucella melitensis - classification</subject><subject>Brucella melitensis - genetics</subject><subject>Brucella melitensis - isolation & purification</subject><subject>Brucellosis - epidemiology</subject><subject>Brucellosis - microbiology</subject><subject>Cluster Analysis</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Minisatellite Repeats</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>Oligonucleotides - genetics</subject><subject>Polymorphism, Genetic</subject><subject>Spain - epidemiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstu1DAYhSMEokNhxxrMArFpii-5OJtK7ahlQFMGMVPEznKcPzOuEjvYSVFelOfBc6HAqivL9ufjc-wTRS8JPiWE8vefptenGKeMxQQ_iiYEFzzOMvz9cTTBuEhjQlh-FD3z_hZjkiRp-jQ6ojhNE06ySfTrstMVtNo2dq2VbJA0FfqyGcMUDPRaoXMjm9Frj2yNlp002m_QbGilQRduUNA0ErXQ6B7MFlr2TmrjUTmi66HpdddAPLdq8OibdFqWYfp5aEtwaBVugjb-Ch3IHq3GTpv1CZqNHbi7A4oWqpctuOBi0ei1NYNqwPbBMLoKNLjOadPvzm1tu85eHISeR09q2Xh4cRiPo5ury9V0Fs8XHz5Oz-exSnjRx6xSWSbTQhWqKlla15BLJlkJgLEkWU2qTFUF4TwFDowCr8usJCCBEFISVrHj6Gyv2w1lC5UCE_I3IvhqpRuFlVr8v2P0RqztnaAFyTLCg8C7g4CzPwbwvWi1372qATt4kWeUZjyhycNkGv42OKUPkyE7x0laBPJkTypnvXdQ3zsnWGzbJUK7xK5dYSXgr_5New__qVMA3h4A6UOZaieN0v4vxzBnNN86fLPnNnq9-akdCOlbcatakXDBBc3ZNu_rPVNLK-TaBZ2bJcWEYcJzmheE_Qab9PLi</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Valdezate, Sylvia</creator><creator>Navarro, Ana</creator><creator>Villalón, Pilar</creator><creator>Carrasco, Gema</creator><creator>Saéz-Nieto, Juan A</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20100801</creationdate><title>Epidemiological and Phylogenetic Analysis of Spanish Human Brucella melitensis Strains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, and rpoB Typing</title><author>Valdezate, Sylvia ; Navarro, Ana ; Villalón, Pilar ; Carrasco, Gema ; Saéz-Nieto, Juan A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-3dc66a59c9cdb35ffe7a3a3bee00a16f1d6cd91885e8e32e8fb6b1eae111b13d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Brucella melitensis</topic><topic>Brucella melitensis - classification</topic><topic>Brucella melitensis - genetics</topic><topic>Brucella melitensis - isolation & purification</topic><topic>Brucellosis - epidemiology</topic><topic>Brucellosis - microbiology</topic><topic>Cluster Analysis</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Minisatellite Repeats</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>Oligonucleotides - genetics</topic><topic>Polymorphism, Genetic</topic><topic>Spain - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valdezate, Sylvia</creatorcontrib><creatorcontrib>Navarro, Ana</creatorcontrib><creatorcontrib>Villalón, Pilar</creatorcontrib><creatorcontrib>Carrasco, Gema</creatorcontrib><creatorcontrib>Saéz-Nieto, Juan A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valdezate, Sylvia</au><au>Navarro, Ana</au><au>Villalón, Pilar</au><au>Carrasco, Gema</au><au>Saéz-Nieto, Juan A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidemiological and Phylogenetic Analysis of Spanish Human Brucella melitensis Strains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, and rpoB Typing</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>48</volume><issue>8</issue><spage>2734</spage><epage>2740</epage><pages>2734-2740</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase β subunit (rpoB) were used to type a human Brucella melitensis population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (37.2 to 93.7%). The population clustered into the following groups: American, with genotypes 47 (1 strain), 48 (13 strains), 53 (12 strains), 55 (2 strains), 80 (1 strain), and a new genotype (2 strains), Western Mediterranean, with genotype 51 (9 strains), and Eastern Mediterranean, with genotypes 42 (60 strains), 43 (4 strains), and 63 (4 strains). Two profession-related and two foodborne acquisitions were confirmed. Distributed throughout Spain, Eastern Mediterranean genotype 42 was the most common (55%). The low MLVA-16 allelic polymorphism (genetic similarity range, 75 to 94%) of the genotype 42 strains suggests that they recently evolved from a common ancestor. rpoB typing grouped the strains as rpoB type 1 (1249-ATG/1309-CTG; 28.7%), rpoB type 2 (1249-ATG/1309-CTA; 62.9%), and rpoB type 3 (1249-ATA/1309-CTG; 8.3%). According to the MLVA-16 results, the population clustered by rpoB type. Given the correlation between B. melitensis MLVA groups and rpoB types (American and rpoB type 1, Eastern Mediterranean and rpoB type 2, and Western Mediterranean and rpoB type 3), the rpoB type could be used as an initial marker for the epidemiological surveillance of brucellosis.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20554816</pmid><doi>10.1128/JCM.00533-10</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial Proteins - genetics Bacterial Typing Techniques Bacteriology Biological and medical sciences Brucella melitensis Brucella melitensis - classification Brucella melitensis - genetics Brucella melitensis - isolation & purification Brucellosis - epidemiology Brucellosis - microbiology Cluster Analysis DNA Fingerprinting DNA, Bacterial - genetics DNA-Directed RNA Polymerases - genetics Fundamental and applied biological sciences. Psychology Genotype Humans Microbiology Minisatellite Repeats Miscellaneous Molecular Epidemiology Oligonucleotides - genetics Polymorphism, Genetic Spain - epidemiology |
| title | Epidemiological and Phylogenetic Analysis of Spanish Human Brucella melitensis Strains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, and rpoB Typing |
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