Chronic Reduction of Endocochlear Potential Reduces Auditory Nerve Activity: Further Confirmation of an Animal Model of Metabolic Presbyacusis

Gerbils aged in quiet show a decline of the endocochlear potential (EP) and elevated auditory nerve compound action potential (CAP) thresholds. However, establishing a direct relationship between an age-related reduction in the EP and changes in the activities of primary auditory neurons is difficul...

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Veröffentlicht in:Journal of the Association for Research in Otolaryngology 2010-09, Vol.11 (3), p.419-434
Hauptverfasser: Lang, Hainan, Jyothi, Vinu, Smythe, Nancy M., Dubno, Judy R., Schulte, Bradley A., Schmiedt, Richard A.
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container_title Journal of the Association for Research in Otolaryngology
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creator Lang, Hainan
Jyothi, Vinu
Smythe, Nancy M.
Dubno, Judy R.
Schulte, Bradley A.
Schmiedt, Richard A.
description Gerbils aged in quiet show a decline of the endocochlear potential (EP) and elevated auditory nerve compound action potential (CAP) thresholds. However, establishing a direct relationship between an age-related reduction in the EP and changes in the activities of primary auditory neurons is difficult owing to the complexity of age-related histological changes in the cochlea. To address this issue, we developed a young gerbil model of “metabolic” presbyacusis that uses an osmotic pump to deliver furosemide into the round window niche for 7 days, resulting in a chronically reduced EP. In this model, the only major histopathologic changes were restricted to the hook region of the cochlea and consisted of loss of strial intermediate cells and massive edema in the lateral wall. The morphological and physiological evidence suggests that the cochlea can adapt to furosemide application over time. The morphology of spiral ganglion cells and hair cells appeared normal throughout the cochlea. CAP responses and EP values in this model are similar to those of quiet-aged ears. The spontaneous activity of single auditory fibers ( n  = 188) was assessed in 15 young gerbils treated with furosemide for 7 days. The percentage of recorded low-spontaneous rate (SR) fibers at characteristic frequencies (CFs) ≥ 6 kHz was significantly lower in furosemide-treated than in control ears. Recovery function tests of CAP responses after prior stimulation also showed a decline in activity of the low-SR population with CFs ≥ 6 kHz in the treated cochleas. A similar loss in the activity of low-SR fiber has been previously shown in quiet-aged gerbils. These results suggest that dysfunction of the cochlear lateral wall and subsequent chronic reduction in the EP can directly affect the activity patterns of primary auditory neurons in a manner similar to that seen in aged gerbils.
doi_str_mv 10.1007/s10162-010-0214-7
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subjects Action Potentials
Adaptation, Physiological
Aging - physiology
Animals
Cochlear Nerve - physiopathology
Disease Models, Animal
Furosemide
Gerbillinae
Hair Cells, Auditory - physiology
Hair Cells, Auditory - ultrastructure
Medicine
Medicine & Public Health
Neurobiology
Neurosciences
Otorhinolaryngology
Presbycusis - chemically induced
Presbycusis - pathology
Presbycusis - physiopathology
Sodium Potassium Chloride Symporter Inhibitors
title Chronic Reduction of Endocochlear Potential Reduces Auditory Nerve Activity: Further Confirmation of an Animal Model of Metabolic Presbyacusis
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