Glycan labeling strategies and their use in identification and quantification
Most methods for the analysis of oligosaccharides from biological sources require a glycan derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore, facilitating detection after chromatographic or electrophoretic separation. Derivatization can also be applied to link...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2010-08, Vol.397 (8), p.3457-3481 |
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| creator | Ruhaak, L. R. Zauner, G. Huhn, C. Bruggink, C. Deelder, A. M. Wuhrer, M. |
| description | Most methods for the analysis of oligosaccharides from biological sources require a glycan derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore, facilitating detection after chromatographic or electrophoretic separation. Derivatization can also be applied to link charged or hydrophobic groups at the reducing end to enhance glycan separation and mass-spectrometric detection. Moreover, derivatization steps such as permethylation aim at stabilizing sialic acid residues, enhancing mass-spectrometric sensitivity, and supporting detailed structural characterization by (tandem) mass spectrometry. Finally, many glycan labels serve as a linker for oligosaccharide attachment to surfaces or carrier proteins, thereby allowing interaction studies with carbohydrate-binding proteins. In this review, various aspects of glycan labeling, separation, and detection strategies are discussed.
Figure
MALDI-FTICR-MS of 2AA-labeled total plasma N-glycans |
| doi_str_mv | 10.1007/s00216-010-3532-z |
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Figure
MALDI-FTICR-MS of 2AA-labeled total plasma N-glycans</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-010-3532-z</identifier><identifier>PMID: 20225063</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Affinity labeling ; Analysis ; Analytical Chemistry ; Animals ; Biochemistry ; Biological ; Carrier Proteins - chemistry ; Carriers ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Desorption ; Electrodiffusion ; Electrophoresis ; Fluorescent Dyes - chemistry ; Food Science ; Glycan ; Glycomics ; Humans ; Ionization ; Laboratory Medicine ; Laser spectroscopy ; Links ; Marking ; Mass Spectrometry ; Methods ; Monitoring/Environmental Analysis ; Oligosaccharides ; Organic acids ; Polysaccharides ; Polysaccharides - chemistry ; Polysaccharides - isolation & purification ; Properties ; Protein Binding ; Proteins ; Review ; Separation ; Separation (Technology) ; Spectra ; Staining and Labeling - methods ; Strategy</subject><ispartof>Analytical and bioanalytical chemistry, 2010-08, Vol.397 (8), p.3457-3481</ispartof><rights>The Author(s) 2010</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-f9dd5fadbd38893399823304da15038f63b2ae5013670ebb378301e52f63e3483</citedby><cites>FETCH-LOGICAL-c573t-f9dd5fadbd38893399823304da15038f63b2ae5013670ebb378301e52f63e3483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-010-3532-z$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-010-3532-z$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20225063$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruhaak, L. R.</creatorcontrib><creatorcontrib>Zauner, G.</creatorcontrib><creatorcontrib>Huhn, C.</creatorcontrib><creatorcontrib>Bruggink, C.</creatorcontrib><creatorcontrib>Deelder, A. M.</creatorcontrib><creatorcontrib>Wuhrer, M.</creatorcontrib><title>Glycan labeling strategies and their use in identification and quantification</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Most methods for the analysis of oligosaccharides from biological sources require a glycan derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore, facilitating detection after chromatographic or electrophoretic separation. Derivatization can also be applied to link charged or hydrophobic groups at the reducing end to enhance glycan separation and mass-spectrometric detection. Moreover, derivatization steps such as permethylation aim at stabilizing sialic acid residues, enhancing mass-spectrometric sensitivity, and supporting detailed structural characterization by (tandem) mass spectrometry. Finally, many glycan labels serve as a linker for oligosaccharide attachment to surfaces or carrier proteins, thereby allowing interaction studies with carbohydrate-binding proteins. In this review, various aspects of glycan labeling, separation, and detection strategies are discussed.
Figure
MALDI-FTICR-MS of 2AA-labeled total plasma N-glycans</description><subject>Affinity labeling</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological</subject><subject>Carrier Proteins - chemistry</subject><subject>Carriers</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Desorption</subject><subject>Electrodiffusion</subject><subject>Electrophoresis</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Food Science</subject><subject>Glycan</subject><subject>Glycomics</subject><subject>Humans</subject><subject>Ionization</subject><subject>Laboratory Medicine</subject><subject>Laser spectroscopy</subject><subject>Links</subject><subject>Marking</subject><subject>Mass Spectrometry</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Oligosaccharides</subject><subject>Organic acids</subject><subject>Polysaccharides</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - isolation & purification</subject><subject>Properties</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Review</subject><subject>Separation</subject><subject>Separation (Technology)</subject><subject>Spectra</subject><subject>Staining and Labeling - methods</subject><subject>Strategy</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNqFklFr1jAUhosobk5_gDdS8EJvOk9ymjS9EcbQTZh4o9chbU67jH7JlrSD7debb90-NpBJLhLO-5yXk-QtivcMDhlA8yUBcCYrYFChQF7dvij2mWSq4lLAy9255nvFm5QuAJhQTL4u9jhwLkDifvHzZLrpjS8n09Hk_FimOZqZRkepNN6W8zm5WC6JSudLZ8nPbnC9mV3wd_rVYh6V3havBjMlene_HxR_vn_7fXxanf06-XF8dFb1osG5GlprxWBsZ1GpFrFtFUeE2homANUgseOGBDCUDVDXYaMQGAmeFcJa4UHxdfW9XLoN2T6PFc2kL6PbmHijg3H6qeLduR7DteYtY4JvDT7dG8RwtVCa9calnqbJeApL0kq2ooEWm_-STZ1vUPMaM_n5WZI1dS1lU0vI6McVHc1E2vkh5Cn7La6PsFWsRbijDv9B5WVp4_rgaXC5_qSBrQ19DClFGnYvwkBvE6PXxOicGL1NjL7NPR8eP-Wu4yEiGeArkLLkR4r6IizR5-99xvUvyKHKoA</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Ruhaak, L. 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Derivatization can also be applied to link charged or hydrophobic groups at the reducing end to enhance glycan separation and mass-spectrometric detection. Moreover, derivatization steps such as permethylation aim at stabilizing sialic acid residues, enhancing mass-spectrometric sensitivity, and supporting detailed structural characterization by (tandem) mass spectrometry. Finally, many glycan labels serve as a linker for oligosaccharide attachment to surfaces or carrier proteins, thereby allowing interaction studies with carbohydrate-binding proteins. In this review, various aspects of glycan labeling, separation, and detection strategies are discussed.
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| subjects | Affinity labeling Analysis Analytical Chemistry Animals Biochemistry Biological Carrier Proteins - chemistry Carriers Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography Desorption Electrodiffusion Electrophoresis Fluorescent Dyes - chemistry Food Science Glycan Glycomics Humans Ionization Laboratory Medicine Laser spectroscopy Links Marking Mass Spectrometry Methods Monitoring/Environmental Analysis Oligosaccharides Organic acids Polysaccharides Polysaccharides - chemistry Polysaccharides - isolation & purification Properties Protein Binding Proteins Review Separation Separation (Technology) Spectra Staining and Labeling - methods Strategy |
| title | Glycan labeling strategies and their use in identification and quantification |
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