Biosafety Assessment of Site-directed Transgene Integration in Human Umbilical Cord–lining Cells

Biosafety and efficacy considerations that impede clinical application of gene therapy could be addressed by nonviral ex vivo cell therapy, utilizing transgenic cells that have been comprehensively pre-evaluated for genotoxic potential and transgene expression. We evaluated the genotoxic potential o...

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Veröffentlicht in:	Molecular therapy 2010-07, Vol.18 (7), p.1346-1356
Hauptverfasser:	Sivalingam, Jaichandran, Krishnan, Shruti, Ng, Wai Har, Lee, Sze Sing, Phan, Toan Thang, Kon, Oi Lian
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blotting, Western Cancer research Cells, Cultured Chromosomes Cloning Electroporation Embryos Epithelial Cells - cytology Epithelial Cells - metabolism Factor VIII - genetics Factor VIII - metabolism Fluorescent Antibody Technique, Indirect Gene therapy Genomes Hemophilia Humans In Situ Hybridization, Fluorescence Karyotyping Medical research Mice Mice, SCID Original Plasmids Reverse Transcriptase Polymerase Chain Reaction Stem cells Transfection Transgenes - genetics Umbilical cord Umbilical Cord - cytology Vectors (Biology)
Online-Zugang:	Volltext
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