The fluorescent protein palette: tools for cellular imaging

This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spec...

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Veröffentlicht in:Chemical Society reviews 2009-10, Vol.38 (10), p.2887-2921
Hauptverfasser: Day, Richard N, Davidson, Michael W
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description This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells, and describe how many different optical methods can be combined with the FPs to provide quantitative measurements in living systems (227 references).
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source MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Biosensing Techniques - methods
cell biology
fluorescence
Fluorescence Recovery After Photobleaching
Fluorescent Dyes
fluorescent proteins
image analysis
Luminescent Measurements
Microscopy, Confocal - methods
Microscopy, Fluorescence - methods
Optics and Photonics - methods
Phylogeny
physiology
Protein Interaction Mapping - methods
quantitative analysis
wood
title The fluorescent protein palette: tools for cellular imaging
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