The fluorescent protein palette: tools for cellular imaging
This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spec...
Gespeichert in:
Veröffentlicht in: | Chemical Society reviews 2009-10, Vol.38 (10), p.2887-2921 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 2921 |
---|---|
container_issue | 10 |
container_start_page | 2887 |
container_title | Chemical Society reviews |
container_volume | 38 |
creator | Day, Richard N Davidson, Michael W |
description | This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells, and describe how many different optical methods can be combined with the FPs to provide quantitative measurements in living systems (227 references). |
doi_str_mv | 10.1039/b901966a |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2910338</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2352436348</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-6e9612da90baadbedf42ebe738343da0935e5ded4eba26fddd9b26381df4033d3</originalsourceid><addsrcrecordid>eNp9kU1LxDAQhoMo7roK_gLpTS_VJJMmGwVBFr9gwct6Dmkz3a10m5q0gv_eLq5fF08zMA8z7zsvIceMnjMK-iLXlGkp7Q4ZMyFpKpQQu2RMgcqUUsZH5CDGl6FjSvJ9MmJaKQaQjcnVYoVJWfc-YCyw6ZI2-A6rJmltjV2Hl0nnfR2T0oekwLruaxuSam2XVbM8JHulrSMebeuEPN_dLmYP6fzp_nF2M08LQUWXStSScWc1za11ObpScMxRwRQEOEs1ZJg5dAJzy2XpnNM5lzBlA0gBHEzI9efets_X6DYyg61NGwYd4d14W5m_k6ZamaV_M1wP3xnOTMjpdkHwrz3GzqyruHFjG_R9NAoEzYRS2UCe_UtyyLgACWL6gxbBxxiw_BbEqNmkYr5SGdCT3wZ-wG0M8AGZWoje</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2352436348</pqid></control><display><type>article</type><title>The fluorescent protein palette: tools for cellular imaging</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Day, Richard N ; Davidson, Michael W</creator><creatorcontrib>Day, Richard N ; Davidson, Michael W</creatorcontrib><description>This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells, and describe how many different optical methods can be combined with the FPs to provide quantitative measurements in living systems (227 references).</description><identifier>ISSN: 0306-0012</identifier><identifier>ISSN: 1460-4744</identifier><identifier>EISSN: 1460-4744</identifier><identifier>DOI: 10.1039/b901966a</identifier><identifier>PMID: 19771335</identifier><language>eng</language><publisher>England</publisher><subject>Biosensing Techniques - methods ; cell biology ; fluorescence ; Fluorescence Recovery After Photobleaching ; Fluorescent Dyes ; fluorescent proteins ; image analysis ; Luminescent Measurements ; Microscopy, Confocal - methods ; Microscopy, Fluorescence - methods ; Optics and Photonics - methods ; Phylogeny ; physiology ; Protein Interaction Mapping - methods ; quantitative analysis ; wood</subject><ispartof>Chemical Society reviews, 2009-10, Vol.38 (10), p.2887-2921</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-6e9612da90baadbedf42ebe738343da0935e5ded4eba26fddd9b26381df4033d3</citedby><cites>FETCH-LOGICAL-c404t-6e9612da90baadbedf42ebe738343da0935e5ded4eba26fddd9b26381df4033d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19771335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Day, Richard N</creatorcontrib><creatorcontrib>Davidson, Michael W</creatorcontrib><title>The fluorescent protein palette: tools for cellular imaging</title><title>Chemical Society reviews</title><addtitle>Chem Soc Rev</addtitle><description>This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells, and describe how many different optical methods can be combined with the FPs to provide quantitative measurements in living systems (227 references).</description><subject>Biosensing Techniques - methods</subject><subject>cell biology</subject><subject>fluorescence</subject><subject>Fluorescence Recovery After Photobleaching</subject><subject>Fluorescent Dyes</subject><subject>fluorescent proteins</subject><subject>image analysis</subject><subject>Luminescent Measurements</subject><subject>Microscopy, Confocal - methods</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Optics and Photonics - methods</subject><subject>Phylogeny</subject><subject>physiology</subject><subject>Protein Interaction Mapping - methods</subject><subject>quantitative analysis</subject><subject>wood</subject><issn>0306-0012</issn><issn>1460-4744</issn><issn>1460-4744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1LxDAQhoMo7roK_gLpTS_VJJMmGwVBFr9gwct6Dmkz3a10m5q0gv_eLq5fF08zMA8z7zsvIceMnjMK-iLXlGkp7Q4ZMyFpKpQQu2RMgcqUUsZH5CDGl6FjSvJ9MmJaKQaQjcnVYoVJWfc-YCyw6ZI2-A6rJmltjV2Hl0nnfR2T0oekwLruaxuSam2XVbM8JHulrSMebeuEPN_dLmYP6fzp_nF2M08LQUWXStSScWc1za11ObpScMxRwRQEOEs1ZJg5dAJzy2XpnNM5lzBlA0gBHEzI9efets_X6DYyg61NGwYd4d14W5m_k6ZamaV_M1wP3xnOTMjpdkHwrz3GzqyruHFjG_R9NAoEzYRS2UCe_UtyyLgACWL6gxbBxxiw_BbEqNmkYr5SGdCT3wZ-wG0M8AGZWoje</recordid><startdate>200910</startdate><enddate>200910</enddate><creator>Day, Richard N</creator><creator>Davidson, Michael W</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200910</creationdate><title>The fluorescent protein palette: tools for cellular imaging</title><author>Day, Richard N ; Davidson, Michael W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-6e9612da90baadbedf42ebe738343da0935e5ded4eba26fddd9b26381df4033d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biosensing Techniques - methods</topic><topic>cell biology</topic><topic>fluorescence</topic><topic>Fluorescence Recovery After Photobleaching</topic><topic>Fluorescent Dyes</topic><topic>fluorescent proteins</topic><topic>image analysis</topic><topic>Luminescent Measurements</topic><topic>Microscopy, Confocal - methods</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Optics and Photonics - methods</topic><topic>Phylogeny</topic><topic>physiology</topic><topic>Protein Interaction Mapping - methods</topic><topic>quantitative analysis</topic><topic>wood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Day, Richard N</creatorcontrib><creatorcontrib>Davidson, Michael W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chemical Society reviews</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Day, Richard N</au><au>Davidson, Michael W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The fluorescent protein palette: tools for cellular imaging</atitle><jtitle>Chemical Society reviews</jtitle><addtitle>Chem Soc Rev</addtitle><date>2009-10</date><risdate>2009</risdate><volume>38</volume><issue>10</issue><spage>2887</spage><epage>2921</epage><pages>2887-2921</pages><issn>0306-0012</issn><issn>1460-4744</issn><eissn>1460-4744</eissn><abstract>This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells, and describe how many different optical methods can be combined with the FPs to provide quantitative measurements in living systems (227 references).</abstract><cop>England</cop><pmid>19771335</pmid><doi>10.1039/b901966a</doi><tpages>35</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0306-0012 |
ispartof | Chemical Society reviews, 2009-10, Vol.38 (10), p.2887-2921 |
issn | 0306-0012 1460-4744 1460-4744 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2910338 |
source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
subjects | Biosensing Techniques - methods cell biology fluorescence Fluorescence Recovery After Photobleaching Fluorescent Dyes fluorescent proteins image analysis Luminescent Measurements Microscopy, Confocal - methods Microscopy, Fluorescence - methods Optics and Photonics - methods Phylogeny physiology Protein Interaction Mapping - methods quantitative analysis wood |
title | The fluorescent protein palette: tools for cellular imaging |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T23%3A38%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20fluorescent%20protein%20palette:%20tools%20for%20cellular%20imaging&rft.jtitle=Chemical%20Society%20reviews&rft.au=Day,%20Richard%20N&rft.date=2009-10&rft.volume=38&rft.issue=10&rft.spage=2887&rft.epage=2921&rft.pages=2887-2921&rft.issn=0306-0012&rft.eissn=1460-4744&rft_id=info:doi/10.1039/b901966a&rft_dat=%3Cproquest_pubme%3E2352436348%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2352436348&rft_id=info:pmid/19771335&rfr_iscdi=true |