Ironing out the wrinkles in the rare biosphere through improved OTU clustering

Summary Deep sequencing of PCR amplicon libraries facilitates the detection of low‐abundance populations in environmental DNA surveys of complex microbial communities. At the same time, deep sequencing can lead to overestimates of microbial diversity through the generation of low‐frequency, error‐pr...

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Veröffentlicht in:	Environmental microbiology 2010-07, Vol.12 (7), p.1889-1898
Hauptverfasser:	Huse, Susan M., Welch, David Mark, Morrison, Hilary G., Sogin, Mitchell L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biodiversity Cluster Analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Environmental Microbiology Escherichia coli - genetics Metagenomics - methods RNA, Ribosomal, 16S - genetics Sequence Alignment - methods Sequence Analysis, DNA Staphylococcus epidermidis - genetics
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