A simple method for generating full length cDNA from low abundance partial genomic clones

PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability may not be sufficient to generate a desired target molecule from two separate regions involving intron /exon boundaries. For th...

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Veröffentlicht in:	BMC molecular biology 2000-11, Vol.1 (1), p.2-2, Article 2
Hauptverfasser:	Wang, Y, Fugaro, J M, Siddiq, F, Goparaju, C M, Lonardo, F, Wali, A, Lechner, J F, Pass, H I
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Cloning DNA DNA sequencing Gene amplification Genes Genetic aspects Genetic research Health aspects Methodology Methods Nucleotide sequencing
Online-Zugang:	Volltext
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