Monitoring viral RNA in infected cells with LNA flow-FISH

We previously showed the feasibility of using locked nucleic acid (LNA) for flow cytometric-fluorescence in situ hybridization (LNA flow-FISH) detection of a target cellular mRNA. Here we demonstrate how the method can be used to monitor viral RNA in infected cells. We compared the results of the LN...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	RNA (Cambridge) 2010-08, Vol.16 (8), p.1679-1685
Hauptverfasser:	Robertson, Kelly L, Verhoeven, Anne Brooks, Thach, Dzung C, Chang, Eddie L
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antiviral Agents Cell Line Cells - chemistry Cricetinae Flow Cytometry - methods Method Nucleic Acid Hybridization - genetics Nucleic Acid Hybridization - methods Nucleic Acids - genetics Physical Phenomena RNA - genetics RNA, Messenger - genetics RNA, Viral - analysis RNA, Viral - genetics Transcription, Genetic Virus Replication - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!