An improved method for generating consistent soluble amyloid-beta oligomer preparations for in vitro neurotoxicity studies

Soluble Aβ oligomers are recognized as playing a key role in Alzheimer's disease (AD) pathophysiology. Despite their significance, many investigators encounter difficulty generating reliable preparations for in vitro and in vivo experiments. Solutions of Aβ are often unstable and soluble confor...

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Veröffentlicht in:	Journal of neuroscience methods 2010-07, Vol.190 (2), p.171-179
Hauptverfasser:	Ryan, Deborah A., Narrow, Wade C., Federoff, Howard J., Bowers, William J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amyloid beta-Peptides - chemical synthesis Amyloid beta-Peptides - metabolism Amyloid beta-Peptides - ultrastructure Amyloid-beta Animals Atomic force microscopy Blotting, Western Cell Death Cell Line, Tumor Cell Survival Cells, Cultured Cerebral Cortex - cytology Cerebral Cortex - metabolism Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Fluorescence Immunoblotting Mice Microscopy, Atomic Force Molecular Weight Neurons - cytology Neurons - metabolism Primary neurons Protein Multimerization Solubility Time Factors
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