Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD
A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C(18) column with a gradient elution sy...
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| Veröffentlicht in: | Pharmacognosy Magazine 2010-04, Vol.6 (22), p.111-115 |
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| creator | Won, Jin Bae Ma, Jin Yeul Um, Young Ran Ma, Choong Je |
| description | A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C(18) column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R(2) > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 mug/ml and 0.13 to 1.11 mug/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%. |
| doi_str_mv | 10.4103/0973-1296.62896 |
| format | Article |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Accuracy Acids Chromatography Herbal medicine High performance liquid chromatography Identification and classification Medicine Methods Original Pharmaceutical chemistry Phytochemicals Quality standards Studies |
| title | Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD |
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