Thermodynamics of Polyethylenimine-DNA Binding and DNA Condensation
In this study, polyethylenimine (PEI) binding to DNA was examined by isothermal titration calorimetry. Two types of binding modes were found to describe the interactions between these polyelectrolytes in buffers and in water. One type of binding involves PEI binding to the DNA groove because the ent...
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| Veröffentlicht in: | Biophysical journal 2010-07, Vol.99 (1), p.201-207 |
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| creator | Utsuno, Kuniharu Uludağ, Hasan |
| description | In this study, polyethylenimine (PEI) binding to DNA was examined by isothermal titration calorimetry. Two types of binding modes were found to describe the interactions between these polyelectrolytes in buffers and in water. One type of binding involves PEI binding to the DNA groove because the enthalpy change of this binding mode is positive, and PEI is deprotonated to bind to DNA. Another likely binding mode involves external binding of PEI to the DNA phosphate backbone, accompanied with DNA condensation. The enthalpy change is negative and PEI is protonated when it binds to DNA in this mode. The intrinsic enthalpy change of first binding mode is 1.1 kJ/mol and −0.88 kJ/mol for the second binding mode. This result implies that the PEI is rearranged from the groove to the phosphate backbone of DNA when DNA is condensed. The mechanism of DNA condensation caused by PEI is discussed in this study. |
| doi_str_mv | 10.1016/j.bpj.2010.04.016 |
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Two types of binding modes were found to describe the interactions between these polyelectrolytes in buffers and in water. One type of binding involves PEI binding to the DNA groove because the enthalpy change of this binding mode is positive, and PEI is deprotonated to bind to DNA. Another likely binding mode involves external binding of PEI to the DNA phosphate backbone, accompanied with DNA condensation. The enthalpy change is negative and PEI is protonated when it binds to DNA in this mode. The intrinsic enthalpy change of first binding mode is 1.1 kJ/mol and −0.88 kJ/mol for the second binding mode. This result implies that the PEI is rearranged from the groove to the phosphate backbone of DNA when DNA is condensed. 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All rights reserved.</rights><rights>Copyright Biophysical Society Jul 7, 2010</rights><rights>2010 by the Biophysical Society.. 2010 Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-66073f6708a2e0e04a68ff1ba0090fcf800a4a7dc7fb04e439565857039b51943</citedby><cites>FETCH-LOGICAL-c608t-66073f6708a2e0e04a68ff1ba0090fcf800a4a7dc7fb04e439565857039b51943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895367/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bpj.2010.04.016$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3550,27924,27925,45995,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20655848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Utsuno, Kuniharu</creatorcontrib><creatorcontrib>Uludağ, Hasan</creatorcontrib><title>Thermodynamics of Polyethylenimine-DNA Binding and DNA Condensation</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>In this study, polyethylenimine (PEI) binding to DNA was examined by isothermal titration calorimetry. Two types of binding modes were found to describe the interactions between these polyelectrolytes in buffers and in water. One type of binding involves PEI binding to the DNA groove because the enthalpy change of this binding mode is positive, and PEI is deprotonated to bind to DNA. Another likely binding mode involves external binding of PEI to the DNA phosphate backbone, accompanied with DNA condensation. The enthalpy change is negative and PEI is protonated when it binds to DNA in this mode. The intrinsic enthalpy change of first binding mode is 1.1 kJ/mol and −0.88 kJ/mol for the second binding mode. This result implies that the PEI is rearranged from the groove to the phosphate backbone of DNA when DNA is condensed. The mechanism of DNA condensation caused by PEI is discussed in this study.</description><subject>Animals</subject><subject>Backbone</subject><subject>Binding</subject><subject>Biochemistry</subject><subject>Biophysics</subject><subject>Calorimetry</subject><subject>Condensation</subject><subject>Condensing</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>Enthalpy</subject><subject>Grooves</subject><subject>Hydrogen-Ion Concentration</subject><subject>Nucleic Acid</subject><subject>Phase Transition</subject><subject>Phosphates</subject><subject>Polyetherimides</subject><subject>Polyethyleneimine - metabolism</subject><subject>Reproducibility of Results</subject><subject>Thermodynamics</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCD-CCIi70kmWc-CtCQirLp1QBh3K2HGfSdZTYWztbaf89jrZUwKGcrBk_80ozDyEvKKwpUPFmWLe7YV1BroGtc-cRWVHOqhJAicdkBQCirFnDT8hpSgMArTjQp-SkAsG5YmpFNldbjFPoDt5MzqYi9MWPMB5w3h5G9G5yHssP3y6K9853zl8XxnfFUm-C79AnM7vgn5EnvRkTPr97z8jPTx-vNl_Ky--fv24uLksrQM2lECDrXkhQpkJAYEaovqetAWigt70CMMzIzsq-BYasbrjgikuom5bThtVn5N0xd7dvJ-ws-jmaUe-im0w86GCc_vvHu62-Dre6Ug2vhcwBr-8CYrjZY5r15JLFcTQewz5pyZmSjZDi_yRTjZSKV5k8f5CkQlIGjVIL-uofdAj76PPJtKDZFWV0gegRsjGkFLG_34-CXqzrQWfrerGugencyTMv_zzM_cRvzRl4ewQw67l1GHWyDr3FzkW0s-6CeyD-F9b1uyU</recordid><startdate>20100707</startdate><enddate>20100707</enddate><creator>Utsuno, Kuniharu</creator><creator>Uludağ, Hasan</creator><general>Elsevier Inc</general><general>Biophysical Society</general><general>The Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7TB</scope><scope>7U5</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20100707</creationdate><title>Thermodynamics of Polyethylenimine-DNA Binding and DNA Condensation</title><author>Utsuno, Kuniharu ; 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Two types of binding modes were found to describe the interactions between these polyelectrolytes in buffers and in water. One type of binding involves PEI binding to the DNA groove because the enthalpy change of this binding mode is positive, and PEI is deprotonated to bind to DNA. Another likely binding mode involves external binding of PEI to the DNA phosphate backbone, accompanied with DNA condensation. The enthalpy change is negative and PEI is protonated when it binds to DNA in this mode. The intrinsic enthalpy change of first binding mode is 1.1 kJ/mol and −0.88 kJ/mol for the second binding mode. This result implies that the PEI is rearranged from the groove to the phosphate backbone of DNA when DNA is condensed. The mechanism of DNA condensation caused by PEI is discussed in this study.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20655848</pmid><doi>10.1016/j.bpj.2010.04.016</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Backbone Binding Biochemistry Biophysics Calorimetry Condensation Condensing Deoxyribonucleic acid DNA DNA - chemistry DNA - metabolism Enthalpy Grooves Hydrogen-Ion Concentration Nucleic Acid Phase Transition Phosphates Polyetherimides Polyethyleneimine - metabolism Reproducibility of Results Thermodynamics |
| title | Thermodynamics of Polyethylenimine-DNA Binding and DNA Condensation |
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