A multiplex calcium assay for identification of GPCR agonists and antagonists

Activation of G(q) protein-coupled receptors can be monitored by measuring the increase in intracellular calcium with fluorescent dyes. Recent advances in fluorescent kinetic plate readers and liquid-handling technology have made it possible to follow these transient changes in intracellular calcium...

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Veröffentlicht in:	Assay and drug development technologies 2010-06, Vol.8 (3), p.367-374
Hauptverfasser:	Liu, Ke, Southall, Noel, Titus, Steve A, Inglese, James, Eskay, Robert L, Shinn, Paul, Austin, Christopher P, Heilig, Markus A, Zheng, Wei
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Calcium - analysis Calcium - metabolism Calibration Chemical reaction, Rate of CHO Cells Cricetinae Cricetulus Cyclic AMP - metabolism Cytosol Dose-Response Relationship, Drug Drug Evaluation, Preclinical Fluorescent Dyes G proteins GTP Health aspects Indicators and Reagents Kinetics Membrane proteins Muscarinic Agonists - pharmacology Muscarinic Antagonists - pharmacology Original Radioligand Assay Receptor, Muscarinic M1 - drug effects Receptors, G-Protein-Coupled - agonists Receptors, G-Protein-Coupled - antagonists & inhibitors
Online-Zugang:	Volltext
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