Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells
Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matrix‐producing o...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2010-08, Vol.94A (2), p.442-449 |
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| container_title | Journal of biomedical materials research. Part A |
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| creator | Hesse, Eric Hefferan, Theresa E. Tarara, James E. Haasper, Carl Meller, Rupert Krettek, Christian Lu, Lichun Yaszemski, Michael J. |
| description | Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matrix‐producing osteoblasts. Collagen type I is of special interest as it is a very important and abundant natural matrix component. The purpose of this study was to investigate whether rat bone marrow stromal cells (rBMSCs) are able to adhere to, to survive, to proliferate and to migrate in collagen type I hydrogels and whether they can adopt an osteoblastic fate. rBMSCs were obtained from rat femora and plated on collagen type I hydrogels. Before harvest by day 7, 14, and 21, hydrogels were fluorescently labeled, cryo‐cut and analyzed by fluorescent‐based and laser scanning confocal microscopy to determine cell proliferation, migration, and viability. Osteogenic differentiation was determined by alkaline phosphatase activity. Collagen type I hydrogels allowed the attachment of rBMSCs to the hydrogel, their proliferation, and migration towards the inner part of the gel. rBMSCs started to differentiate into osteoblasts as determined by an increase in alkaline phosphatase activity after two weeks in culture. This study therefore suggests that collagen type I hydrogels could be useful for musculoskeletal regenerative therapies. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res 2010 |
| doi_str_mv | 10.1002/jbm.a.32696 |
| format | Article |
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Collagen type I is of special interest as it is a very important and abundant natural matrix component. The purpose of this study was to investigate whether rat bone marrow stromal cells (rBMSCs) are able to adhere to, to survive, to proliferate and to migrate in collagen type I hydrogels and whether they can adopt an osteoblastic fate. rBMSCs were obtained from rat femora and plated on collagen type I hydrogels. Before harvest by day 7, 14, and 21, hydrogels were fluorescently labeled, cryo‐cut and analyzed by fluorescent‐based and laser scanning confocal microscopy to determine cell proliferation, migration, and viability. Osteogenic differentiation was determined by alkaline phosphatase activity. Collagen type I hydrogels allowed the attachment of rBMSCs to the hydrogel, their proliferation, and migration towards the inner part of the gel. rBMSCs started to differentiate into osteoblasts as determined by an increase in alkaline phosphatase activity after two weeks in culture. This study therefore suggests that collagen type I hydrogels could be useful for musculoskeletal regenerative therapies. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res 2010</description><identifier>ISSN: 1549-3296</identifier><identifier>ISSN: 1552-4965</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.32696</identifier><identifier>PMID: 20186733</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biocompatible Materials - metabolism ; Biological and medical sciences ; Bone Marrow Cells - cytology ; Bone Marrow Cells - physiology ; bone marrow stromal cells ; bone regeneration ; Cattle ; Cell Differentiation - physiology ; cell migration ; Cell Movement - physiology ; Cell Proliferation ; Cells, Cultured ; Collagen Type I - metabolism ; collagen type I hydrogel ; Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism ; Male ; Materials Testing ; Medical sciences ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Osteogenesis - physiology ; osteogenic differentiation ; Rats ; Rats, Sprague-Dawley ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matrix‐producing osteoblasts. Collagen type I is of special interest as it is a very important and abundant natural matrix component. The purpose of this study was to investigate whether rat bone marrow stromal cells (rBMSCs) are able to adhere to, to survive, to proliferate and to migrate in collagen type I hydrogels and whether they can adopt an osteoblastic fate. rBMSCs were obtained from rat femora and plated on collagen type I hydrogels. Before harvest by day 7, 14, and 21, hydrogels were fluorescently labeled, cryo‐cut and analyzed by fluorescent‐based and laser scanning confocal microscopy to determine cell proliferation, migration, and viability. Osteogenic differentiation was determined by alkaline phosphatase activity. Collagen type I hydrogels allowed the attachment of rBMSCs to the hydrogel, their proliferation, and migration towards the inner part of the gel. rBMSCs started to differentiate into osteoblasts as determined by an increase in alkaline phosphatase activity after two weeks in culture. This study therefore suggests that collagen type I hydrogels could be useful for musculoskeletal regenerative therapies. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res 2010</description><subject>Animals</subject><subject>Biocompatible Materials - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - physiology</subject><subject>bone marrow stromal cells</subject><subject>bone regeneration</subject><subject>Cattle</subject><subject>Cell Differentiation - physiology</subject><subject>cell migration</subject><subject>Cell Movement - physiology</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Collagen Type I - metabolism</subject><subject>collagen type I hydrogel</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism</subject><subject>Male</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis - physiology</subject><subject>osteogenic differentiation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><issn>1549-3296</issn><issn>1552-4965</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1vFCEYxidGYz_05N1wMR7srHwMDFxMdK1rm6qJqfFIGAa2VGZYYbbr_Pey3Y_aiz0BeX887_PyUBQvEJwgCPHb66abqAnBTLBHxSGiFJeVYPTxel-JkmDBDoqjlK4zzCDFT4sDDBFnNSGHxTgN3qu56cEwLgw4A1djG8PceKC8D6sEOjePanChPwGLGLyzZndUfQtCGkyme6dB62yumX5wt3UQLMgkaEJvQKdiDCuQhhg65YE23qdnxROrfDLPt-tx8ePT6eX0c3nxbXY2fX9RaoYhKysrDG0bA7nWlracVxAZaGssWsVoZTVBWleMq5pQziFpGm0R1dqgVmNWc3JcvNvoLpZNZ1qdHUbl5SK67GqUQTl5v9K7KzkPNxJzgTgRWeD1ViCG30uTBtm5tB5B9SYsk-ScQFTV-TkfJJmggkL4sGYWqyBEDGXyzYbUMaQUjd07R1Cu85c5f6nkbf6ZfvnvsHt2F3gGXm0BlbTyNqpeu3THkexO4CpzaMOtnDfj_3rK8w9fds3LzR2Xf8Wf_R0Vf8ncu6by59eZ_Dg7n0Hx_VIK8hc7QdpX</recordid><startdate>201008</startdate><enddate>201008</enddate><creator>Hesse, Eric</creator><creator>Hefferan, Theresa E.</creator><creator>Tarara, James E.</creator><creator>Haasper, Carl</creator><creator>Meller, Rupert</creator><creator>Krettek, Christian</creator><creator>Lu, Lichun</creator><creator>Yaszemski, Michael J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7QP</scope><scope>5PM</scope></search><sort><creationdate>201008</creationdate><title>Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells</title><author>Hesse, Eric ; Hefferan, Theresa E. ; Tarara, James E. ; Haasper, Carl ; Meller, Rupert ; Krettek, Christian ; Lu, Lichun ; Yaszemski, Michael J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6206-4f9e5dbe08ccf5d88401e0f729da654fc31cc468a7358803bbcf15cce1dc26783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biocompatible Materials - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - physiology</topic><topic>bone marrow stromal cells</topic><topic>bone regeneration</topic><topic>Cattle</topic><topic>Cell Differentiation - physiology</topic><topic>cell migration</topic><topic>Cell Movement - physiology</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Collagen Type I - metabolism</topic><topic>collagen type I hydrogel</topic><topic>Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism</topic><topic>Male</topic><topic>Materials Testing</topic><topic>Medical sciences</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Osteogenesis - physiology</topic><topic>osteogenic differentiation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Surgery (general aspects). 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| subjects | Animals Biocompatible Materials - metabolism Biological and medical sciences Bone Marrow Cells - cytology Bone Marrow Cells - physiology bone marrow stromal cells bone regeneration Cattle Cell Differentiation - physiology cell migration Cell Movement - physiology Cell Proliferation Cells, Cultured Collagen Type I - metabolism collagen type I hydrogel Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism Male Materials Testing Medical sciences Osteoblasts - cytology Osteoblasts - metabolism Osteogenesis - physiology osteogenic differentiation Rats Rats, Sprague-Dawley Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments |
| title | Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells |
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