A Specificity Switch in Selected Cre Recombinase Variants Is Mediated by Macromolecular Plasticity and Water

The basis for the altered DNA specificities of two Cre recombinase variants, obtained by mutation and selection, was revealed by their cocrystal structures. The proteins share similar substitutions but differ in their preferences for the natural LoxP substrate and an engineered substrate that is ina...

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Veröffentlicht in:	Chemistry & biology 2003-11, Vol.10 (11), p.1085-1094
Hauptverfasser:	Baldwin, Enoch P., Martin, Shelley S., Abel, Jonas, Gelato, Kathy A., Kim, Hanseong, Schultz, Peter G., Santoro, Stephen W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Integrases - chemistry Integrases - metabolism Models, Molecular PARTICLE ACCELERATORS PLASTICITY SPECIFICITY STANFORD LINEAR ACCELERATOR CENTER STANFORD SYNCHROTRON RADIATION LABORATORY SYNCHROTRON RADIATION Viral Proteins - chemistry Viral Proteins - metabolism WATER Water - chemistry
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