Histone H1 null vertebrate cells exhibit altered nucleosome architecture
In eukaryotic nuclei, DNA is wrapped around an octamer of core histones to form nucleosomes, and chromatin fibers are thought to be stabilized by linker histones of the H1 type. Higher eukaryotes express multiple variants of histone H1; chickens possess six H1 variants. Here, we generated and analyz...
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| Veröffentlicht in: | Nucleic acids research 2010-06, Vol.38 (11), p.3533-3545 |
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| creator | Hashimoto, Hideharu Takami, Yasunari Sonoda, Eiichiro Iwasaki, Tomohito Iwano, Hidetomo Tachibana, Makoto Takeda, Shunichi Nakayama, Tatsuo Kimura, Hiroshi Shinkai, Yoichi |
| description | In eukaryotic nuclei, DNA is wrapped around an octamer of core histones to form nucleosomes, and chromatin fibers are thought to be stabilized by linker histones of the H1 type. Higher eukaryotes express multiple variants of histone H1; chickens possess six H1 variants. Here, we generated and analyzed the phenotype of a complete deletion of histone H1 genes in chicken cells. The H1-null cells showed decreased global nucleosome spacing, expanded nuclear volumes, and increased chromosome aberration rates, although proper mitotic chromatin structure appeared to be maintained. Expression array analysis revealed that the transcription of multiple genes was affected and was mostly downregulated in histone H1-deficient cells. This report describes the first histone H1 complete knockout cells in vertebrates and suggests that linker histone H1, while not required for mitotic chromatin condensation, plays important roles in nucleosome spacing and interphase chromatin compaction and acts as a global transcription regulator. |
| doi_str_mv | 10.1093/nar/gkq076 |
| format | Article |
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Higher eukaryotes express multiple variants of histone H1; chickens possess six H1 variants. Here, we generated and analyzed the phenotype of a complete deletion of histone H1 genes in chicken cells. The H1-null cells showed decreased global nucleosome spacing, expanded nuclear volumes, and increased chromosome aberration rates, although proper mitotic chromatin structure appeared to be maintained. Expression array analysis revealed that the transcription of multiple genes was affected and was mostly downregulated in histone H1-deficient cells. This report describes the first histone H1 complete knockout cells in vertebrates and suggests that linker histone H1, while not required for mitotic chromatin condensation, plays important roles in nucleosome spacing and interphase chromatin compaction and acts as a global transcription regulator.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/gkq076</identifier><identifier>PMID: 20156997</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Cell Cycle ; Cell Line ; Chickens - genetics ; Chickens - growth & development ; Chickens - metabolism ; Chromatin - ultrastructure ; Chromosome Aberrations ; Gene Regulation, Chromatin and Epigenetics ; Histones - genetics ; Histones - physiology ; Interphase - genetics ; Mutation ; Nucleosomes - chemistry ; Transcription, Genetic</subject><ispartof>Nucleic acids research, 2010-06, Vol.38 (11), p.3533-3545</ispartof><rights>The Author(s) 2010. Published by Oxford University Press. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-631dd3ce2c123a51a09bec857c4e109793b5e9047aebece4aff15e3350c6cd213</citedby><cites>FETCH-LOGICAL-c543t-631dd3ce2c123a51a09bec857c4e109793b5e9047aebece4aff15e3350c6cd213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887950/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887950/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20156997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashimoto, Hideharu</creatorcontrib><creatorcontrib>Takami, Yasunari</creatorcontrib><creatorcontrib>Sonoda, Eiichiro</creatorcontrib><creatorcontrib>Iwasaki, Tomohito</creatorcontrib><creatorcontrib>Iwano, Hidetomo</creatorcontrib><creatorcontrib>Tachibana, Makoto</creatorcontrib><creatorcontrib>Takeda, Shunichi</creatorcontrib><creatorcontrib>Nakayama, Tatsuo</creatorcontrib><creatorcontrib>Kimura, Hiroshi</creatorcontrib><creatorcontrib>Shinkai, Yoichi</creatorcontrib><title>Histone H1 null vertebrate cells exhibit altered nucleosome architecture</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>In eukaryotic nuclei, DNA is wrapped around an octamer of core histones to form nucleosomes, and chromatin fibers are thought to be stabilized by linker histones of the H1 type. Higher eukaryotes express multiple variants of histone H1; chickens possess six H1 variants. Here, we generated and analyzed the phenotype of a complete deletion of histone H1 genes in chicken cells. The H1-null cells showed decreased global nucleosome spacing, expanded nuclear volumes, and increased chromosome aberration rates, although proper mitotic chromatin structure appeared to be maintained. Expression array analysis revealed that the transcription of multiple genes was affected and was mostly downregulated in histone H1-deficient cells. This report describes the first histone H1 complete knockout cells in vertebrates and suggests that linker histone H1, while not required for mitotic chromatin condensation, plays important roles in nucleosome spacing and interphase chromatin compaction and acts as a global transcription regulator.</description><subject>Animals</subject><subject>Cell Cycle</subject><subject>Cell Line</subject><subject>Chickens - genetics</subject><subject>Chickens - growth & development</subject><subject>Chickens - metabolism</subject><subject>Chromatin - ultrastructure</subject><subject>Chromosome Aberrations</subject><subject>Gene Regulation, Chromatin and Epigenetics</subject><subject>Histones - genetics</subject><subject>Histones - physiology</subject><subject>Interphase - genetics</subject><subject>Mutation</subject><subject>Nucleosomes - chemistry</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpyG4-Lv0BrW-FgBNJI1nWpRCWpFsI5JDsWcjyeFet10okeWn_fbxsGtpTTgMzDy_z8hDyidFLRjVcDTZerX89U1V9IHMGFS-FrvhHMqdAZcmoqGfkJKWflDLBpDgmM06ZrLRWc7Jc-pTDgMWSFcPY98UOY8Ym2oyFw75PBf7e-MbnwvYZI7YT5XoMKWyxsNFtfEaXx4hn5KizfcLz13lKVrc3j4tleXf__cfi-q50UkAuK2BtCw65YxysZJbqBl0tlRM4lVEaGomaCmVx2qOwXcckAkjqKtdyBqfk2yH3aWy22DoccrS9eYp-a-MfE6w3_18GvzHrsDO8rpWWdAr4-hoQw_OIKZutT_uqdsAwJqOE1KCY4O-TAKBA8T15cSBdDClF7N7-YdTsHZnJkTk4muDP_zZ4Q_9KmYAvB6Czwdh19MmsHqYrUFZLLRSHF97JmM4</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Hashimoto, Hideharu</creator><creator>Takami, Yasunari</creator><creator>Sonoda, Eiichiro</creator><creator>Iwasaki, Tomohito</creator><creator>Iwano, Hidetomo</creator><creator>Tachibana, Makoto</creator><creator>Takeda, Shunichi</creator><creator>Nakayama, Tatsuo</creator><creator>Kimura, Hiroshi</creator><creator>Shinkai, Yoichi</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20100601</creationdate><title>Histone H1 null vertebrate cells exhibit altered nucleosome architecture</title><author>Hashimoto, Hideharu ; Takami, Yasunari ; Sonoda, Eiichiro ; Iwasaki, Tomohito ; Iwano, Hidetomo ; Tachibana, Makoto ; Takeda, Shunichi ; Nakayama, Tatsuo ; Kimura, Hiroshi ; Shinkai, Yoichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c543t-631dd3ce2c123a51a09bec857c4e109793b5e9047aebece4aff15e3350c6cd213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cell Cycle</topic><topic>Cell Line</topic><topic>Chickens - genetics</topic><topic>Chickens - growth & development</topic><topic>Chickens - metabolism</topic><topic>Chromatin - ultrastructure</topic><topic>Chromosome Aberrations</topic><topic>Gene Regulation, Chromatin and Epigenetics</topic><topic>Histones - genetics</topic><topic>Histones - physiology</topic><topic>Interphase - genetics</topic><topic>Mutation</topic><topic>Nucleosomes - chemistry</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashimoto, Hideharu</creatorcontrib><creatorcontrib>Takami, Yasunari</creatorcontrib><creatorcontrib>Sonoda, Eiichiro</creatorcontrib><creatorcontrib>Iwasaki, Tomohito</creatorcontrib><creatorcontrib>Iwano, Hidetomo</creatorcontrib><creatorcontrib>Tachibana, Makoto</creatorcontrib><creatorcontrib>Takeda, Shunichi</creatorcontrib><creatorcontrib>Nakayama, Tatsuo</creatorcontrib><creatorcontrib>Kimura, Hiroshi</creatorcontrib><creatorcontrib>Shinkai, Yoichi</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashimoto, Hideharu</au><au>Takami, Yasunari</au><au>Sonoda, Eiichiro</au><au>Iwasaki, Tomohito</au><au>Iwano, Hidetomo</au><au>Tachibana, Makoto</au><au>Takeda, Shunichi</au><au>Nakayama, Tatsuo</au><au>Kimura, Hiroshi</au><au>Shinkai, Yoichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histone H1 null vertebrate cells exhibit altered nucleosome architecture</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>38</volume><issue>11</issue><spage>3533</spage><epage>3545</epage><pages>3533-3545</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>In eukaryotic nuclei, DNA is wrapped around an octamer of core histones to form nucleosomes, and chromatin fibers are thought to be stabilized by linker histones of the H1 type. Higher eukaryotes express multiple variants of histone H1; chickens possess six H1 variants. Here, we generated and analyzed the phenotype of a complete deletion of histone H1 genes in chicken cells. The H1-null cells showed decreased global nucleosome spacing, expanded nuclear volumes, and increased chromosome aberration rates, although proper mitotic chromatin structure appeared to be maintained. Expression array analysis revealed that the transcription of multiple genes was affected and was mostly downregulated in histone H1-deficient cells. 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| subjects | Animals Cell Cycle Cell Line Chickens - genetics Chickens - growth & development Chickens - metabolism Chromatin - ultrastructure Chromosome Aberrations Gene Regulation, Chromatin and Epigenetics Histones - genetics Histones - physiology Interphase - genetics Mutation Nucleosomes - chemistry Transcription, Genetic |
| title | Histone H1 null vertebrate cells exhibit altered nucleosome architecture |
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