Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.)
Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic...
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| Veröffentlicht in: | International journal of molecular sciences 2010-05, Vol.11 (5), p.2010-2016 |
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| container_title | International journal of molecular sciences |
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| creator | Pirseyedi, Seyed Mostafa Valizadehghan, Sahar Mardi, Mohsen Ghaffari, Mohammad Reza Mahmoodi, Parvaneh Zahravi, Mehdi Zeinalabedini, Mehrshad Nekoui, Seyed Mojtaba Khayam |
| description | Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future. |
| doi_str_mv | 10.3390/ijms11052010 |
| format | Article |
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Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms11052010</identifier><identifier>PMID: 20559498</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Agricultural biotechnology ; Alleles ; Cloning ; Genetic diversity ; Genetic Loci ; Genomes ; Genomic Library ; Lythraceae - genetics ; Microsatellite Repeats ; Morphology ; Polymorphism, Genetic</subject><ispartof>International journal of molecular sciences, 2010-05, Vol.11 (5), p.2010-2016</ispartof><rights>Copyright MDPI AG 2010</rights><rights>2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-1096bec8b2b354714d882a1d9356c49d3f1f5d6f7a735e4e88cf480db29c3d163</citedby><cites>FETCH-LOGICAL-c411t-1096bec8b2b354714d882a1d9356c49d3f1f5d6f7a735e4e88cf480db29c3d163</cites><orcidid>0000-0002-9627-2363</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885090/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885090/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20559498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pirseyedi, Seyed Mostafa</creatorcontrib><creatorcontrib>Valizadehghan, Sahar</creatorcontrib><creatorcontrib>Mardi, Mohsen</creatorcontrib><creatorcontrib>Ghaffari, Mohammad Reza</creatorcontrib><creatorcontrib>Mahmoodi, Parvaneh</creatorcontrib><creatorcontrib>Zahravi, Mehdi</creatorcontrib><creatorcontrib>Zeinalabedini, Mehrshad</creatorcontrib><creatorcontrib>Nekoui, Seyed Mojtaba Khayam</creatorcontrib><title>Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.)</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future.</description><subject>Agricultural biotechnology</subject><subject>Alleles</subject><subject>Cloning</subject><subject>Genetic diversity</subject><subject>Genetic Loci</subject><subject>Genomes</subject><subject>Genomic Library</subject><subject>Lythraceae - genetics</subject><subject>Microsatellite Repeats</subject><subject>Morphology</subject><subject>Polymorphism, Genetic</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1LxDAQxYMoft88S8CDCq5OkqZNLoKIHwsLetBzSNNUs7bJmrSC_vV22VVWT5OZ-fF4k4fQAYFzxiRcuGmbCAFOgcAa2iYZpSOAvFhfeW-hnZSmAJRRLjfRFgXOZSbFNirHKTS6c8Fj7StsXnXUprPRfS2GocY-fNgGt87EkHRnm8Z1Frc6vtmYsPN4Flr7ErUfdvjksffOaLzo-xZPzk_30Eatm2T3l3UXPd_ePF3fjyYPd-Prq8nIZIR0IwIyL60RJS0ZzwqSVUJQTSrJeG4yWbGa1LzK60IXjNvMCmHqTEBVUmlYRXK2iy4XurO-bG1lrO-ibtQsusHspwraqb8b717VS_hQVAgOEgaB46VADO-9TZ1qXTLDwdrb0CdVMMa4oHxOHv0jp6GPfrhOEU5zACjo3NDZgpr_XIq2_vVCQM2zU6vZDfjhqv9f-Ccs9g3iN5ZT</recordid><startdate>20100503</startdate><enddate>20100503</enddate><creator>Pirseyedi, Seyed Mostafa</creator><creator>Valizadehghan, Sahar</creator><creator>Mardi, Mohsen</creator><creator>Ghaffari, Mohammad Reza</creator><creator>Mahmoodi, Parvaneh</creator><creator>Zahravi, Mehdi</creator><creator>Zeinalabedini, Mehrshad</creator><creator>Nekoui, Seyed Mojtaba Khayam</creator><general>MDPI AG</general><general>Molecular Diversity Preservation International (MDPI)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9627-2363</orcidid></search><sort><creationdate>20100503</creationdate><title>Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.)</title><author>Pirseyedi, Seyed Mostafa ; Valizadehghan, Sahar ; Mardi, Mohsen ; Ghaffari, Mohammad Reza ; Mahmoodi, Parvaneh ; Zahravi, Mehdi ; Zeinalabedini, Mehrshad ; Nekoui, Seyed Mojtaba Khayam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-1096bec8b2b354714d882a1d9356c49d3f1f5d6f7a735e4e88cf480db29c3d163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Agricultural biotechnology</topic><topic>Alleles</topic><topic>Cloning</topic><topic>Genetic diversity</topic><topic>Genetic Loci</topic><topic>Genomes</topic><topic>Genomic Library</topic><topic>Lythraceae - genetics</topic><topic>Microsatellite Repeats</topic><topic>Morphology</topic><topic>Polymorphism, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pirseyedi, Seyed Mostafa</creatorcontrib><creatorcontrib>Valizadehghan, Sahar</creatorcontrib><creatorcontrib>Mardi, Mohsen</creatorcontrib><creatorcontrib>Ghaffari, Mohammad Reza</creatorcontrib><creatorcontrib>Mahmoodi, Parvaneh</creatorcontrib><creatorcontrib>Zahravi, Mehdi</creatorcontrib><creatorcontrib>Zeinalabedini, Mehrshad</creatorcontrib><creatorcontrib>Nekoui, Seyed Mojtaba Khayam</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pirseyedi, Seyed Mostafa</au><au>Valizadehghan, Sahar</au><au>Mardi, Mohsen</au><au>Ghaffari, Mohammad Reza</au><au>Mahmoodi, Parvaneh</au><au>Zahravi, Mehdi</au><au>Zeinalabedini, Mehrshad</au><au>Nekoui, Seyed Mojtaba Khayam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.)</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2010-05-03</date><risdate>2010</risdate><volume>11</volume><issue>5</issue><spage>2010</spage><epage>2016</epage><pages>2010-2016</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. 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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Agricultural biotechnology Alleles Cloning Genetic diversity Genetic Loci Genomes Genomic Library Lythraceae - genetics Microsatellite Repeats Morphology Polymorphism, Genetic |
| title | Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.) |
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