Genetically Encoded ε-N-Methyl Lysine in Mammalian Cells

Newly evolved pyrrolysyl‐tRNA synthetases from M. barkeri and M. mazei tRNA${{{\rm Pyl}\hfill \atop {\rm CUA}\hfill}}}$ were used to site‐specifically incorporate photocaged methyl lysine into proteins in both E. coli and mammalian cells with high fidelity and efficiency. A GFP mutant containing pho...

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