Prostaglandin-E2 is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner
Summary Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin‐E2 (PGE2). While keratinocytes are a major PGE2 source, epidermal melanocytes (EM) also express PGE2‐production machinery. It is unclear whether EM‐produced PGE2 contributes to UVR‐induced skin...
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| Veröffentlicht in: | Pigment cell and melanoma research 2010-06, Vol.23 (3), p.394-403 |
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| creator | Gledhill, Karl Rhodes, Lesley E. Brownrigg, Margaret Haylett, Ann K. Masoodi, Mojgan Thody, Anthony J. Nicolaou, Anna Tobin, Desmond J. |
| description | Summary
Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin‐E2 (PGE2). While keratinocytes are a major PGE2 source, epidermal melanocytes (EM) also express PGE2‐production machinery. It is unclear whether EM‐produced PGE2 contributes to UVR‐induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype‐1 and ‐4 donors, followed by assessment of PGE2 production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase‐A2, cyclooxygenase‐1, cytoplasmic prostaglandin‐E synthase and microsomal prostaglandin‐E synthase‐1, ‐2. Epidermal melanocytes produced PGE2 under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase‐2 (COX‐2) mRNA and a selective COX‐2 inhibitor (NS‐398) reduced PGE2 production. Ultraviolet B‐induced PGE2 production was positively correlated with skin phototype‐1, despite variability between individual EM donors. By contrast, there was no correlation between PGE2 production by EM and their melanogenic status. Thus, EM may contribute to UVR‐induced erythema, with role of donor skin phototype more important than their melanogenic status.
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| doi_str_mv | 10.1111/j.1755-148X.2010.00696.x |
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| fullrecord | <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2881306</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>PCMR696</sourcerecordid><originalsourceid>FETCH-LOGICAL-i3886-96e8dcbf8a2ef90bb5e0362905e0001135957d5f3f9e95f4cd58db7d3e832e3</originalsourceid><addsrcrecordid>eNpVUdtu1DAQtRCIlsIvIP9Atr6sE-cBJFi1BdHCirt4GTnxZOslcSI7C7t8PQ5bIpiHmdGciyUfQihnC57qfLvghVIZX-qvC8HSlbG8zBf7e-R0Bu7Pe8FPyKMYt4nEVCkfkhPBhMyXS3FKfq1DH0ezaY23zmcXgrpIh9DbXY2WVgdq7K4d6e2uM57i4CyGzrS0wyTo68OIkTpPA8ah9xHp2NNPn19OJ3PH2aDH6GLmvMUBU_MjTV4ew2PyoDFtxCd384x8uLz4uHqVXb-7er16cZ05qXWelTlqW1eNNgKbklWVQiZzUbI0GeNcqlIVVjWyKbFUzbK2StuqsBK1FCjPyPOj67CrOrR1ej-YFobgOhMO0BsH_yPe3cKm_wFCay5Zngye_mswK__-YSI8OxJ-uhYPM84ZTFnBFqYYYIoEpqzgT1awh_Xq5n3akj476l0ccT_rTfgOeSELBV_eXsEbdcP4pV7DN_kbos2axA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Prostaglandin-E2 is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Gledhill, Karl ; Rhodes, Lesley E. ; Brownrigg, Margaret ; Haylett, Ann K. ; Masoodi, Mojgan ; Thody, Anthony J. ; Nicolaou, Anna ; Tobin, Desmond J.</creator><creatorcontrib>Gledhill, Karl ; Rhodes, Lesley E. ; Brownrigg, Margaret ; Haylett, Ann K. ; Masoodi, Mojgan ; Thody, Anthony J. ; Nicolaou, Anna ; Tobin, Desmond J.</creatorcontrib><description>Summary
Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin‐E2 (PGE2). While keratinocytes are a major PGE2 source, epidermal melanocytes (EM) also express PGE2‐production machinery. It is unclear whether EM‐produced PGE2 contributes to UVR‐induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype‐1 and ‐4 donors, followed by assessment of PGE2 production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase‐A2, cyclooxygenase‐1, cytoplasmic prostaglandin‐E synthase and microsomal prostaglandin‐E synthase‐1, ‐2. Epidermal melanocytes produced PGE2 under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase‐2 (COX‐2) mRNA and a selective COX‐2 inhibitor (NS‐398) reduced PGE2 production. Ultraviolet B‐induced PGE2 production was positively correlated with skin phototype‐1, despite variability between individual EM donors. By contrast, there was no correlation between PGE2 production by EM and their melanogenic status. Thus, EM may contribute to UVR‐induced erythema, with role of donor skin phototype more important than their melanogenic status.
View the pubcast on this paper at http://www.scivee.tv/node/18147</description><identifier>ISSN: 1755-1471</identifier><identifier>EISSN: 1755-148X</identifier><identifier>DOI: 10.1111/j.1755-148X.2010.00696.x</identifier><identifier>PMID: 20236442</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Arachidonic Acid - pharmacology ; cyclooxygenase ; Dinoprostone - biosynthesis ; Epidermal Cells ; epidermal melanocyte ; Female ; Humans ; Hydrogen Peroxide - pharmacology ; Indomethacin - pharmacology ; Intramolecular Oxidoreductases - metabolism ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Keratinocytes - radiation effects ; Male ; Melanins - biosynthesis ; Melanocytes - drug effects ; Melanocytes - enzymology ; Melanocytes - metabolism ; Melanocytes - radiation effects ; melanogenesis ; Membrane Glycoproteins - metabolism ; Monophenol Monooxygenase - metabolism ; Nitrobenzenes ; Original ; Oxidoreductases - metabolism ; prostaglandin E2 ; skin phototype ; Sulfonamides ; ultraviolet radiation ; Ultraviolet Rays ; Young Adult</subject><ispartof>Pigment cell and melanoma research, 2010-06, Vol.23 (3), p.394-403</ispartof><rights>2010 John Wiley & Sons A/S</rights><rights>2010 Blackwell Munksgaard</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1755-148X.2010.00696.x$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1755-148X.2010.00696.x$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20236442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gledhill, Karl</creatorcontrib><creatorcontrib>Rhodes, Lesley E.</creatorcontrib><creatorcontrib>Brownrigg, Margaret</creatorcontrib><creatorcontrib>Haylett, Ann K.</creatorcontrib><creatorcontrib>Masoodi, Mojgan</creatorcontrib><creatorcontrib>Thody, Anthony J.</creatorcontrib><creatorcontrib>Nicolaou, Anna</creatorcontrib><creatorcontrib>Tobin, Desmond J.</creatorcontrib><title>Prostaglandin-E2 is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner</title><title>Pigment cell and melanoma research</title><addtitle>Pigment Cell Melanoma Res</addtitle><description>Summary
Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin‐E2 (PGE2). While keratinocytes are a major PGE2 source, epidermal melanocytes (EM) also express PGE2‐production machinery. It is unclear whether EM‐produced PGE2 contributes to UVR‐induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype‐1 and ‐4 donors, followed by assessment of PGE2 production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase‐A2, cyclooxygenase‐1, cytoplasmic prostaglandin‐E synthase and microsomal prostaglandin‐E synthase‐1, ‐2. Epidermal melanocytes produced PGE2 under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase‐2 (COX‐2) mRNA and a selective COX‐2 inhibitor (NS‐398) reduced PGE2 production. Ultraviolet B‐induced PGE2 production was positively correlated with skin phototype‐1, despite variability between individual EM donors. By contrast, there was no correlation between PGE2 production by EM and their melanogenic status. Thus, EM may contribute to UVR‐induced erythema, with role of donor skin phototype more important than their melanogenic status.
View the pubcast on this paper at http://www.scivee.tv/node/18147</description><subject>Adult</subject><subject>Arachidonic Acid - pharmacology</subject><subject>cyclooxygenase</subject><subject>Dinoprostone - biosynthesis</subject><subject>Epidermal Cells</subject><subject>epidermal melanocyte</subject><subject>Female</subject><subject>Humans</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Indomethacin - pharmacology</subject><subject>Intramolecular Oxidoreductases - metabolism</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - radiation effects</subject><subject>Male</subject><subject>Melanins - biosynthesis</subject><subject>Melanocytes - drug effects</subject><subject>Melanocytes - enzymology</subject><subject>Melanocytes - metabolism</subject><subject>Melanocytes - radiation effects</subject><subject>melanogenesis</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Monophenol Monooxygenase - metabolism</subject><subject>Nitrobenzenes</subject><subject>Original</subject><subject>Oxidoreductases - metabolism</subject><subject>prostaglandin E2</subject><subject>skin phototype</subject><subject>Sulfonamides</subject><subject>ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><subject>Young Adult</subject><issn>1755-1471</issn><issn>1755-148X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNpVUdtu1DAQtRCIlsIvIP9Atr6sE-cBJFi1BdHCirt4GTnxZOslcSI7C7t8PQ5bIpiHmdGciyUfQihnC57qfLvghVIZX-qvC8HSlbG8zBf7e-R0Bu7Pe8FPyKMYt4nEVCkfkhPBhMyXS3FKfq1DH0ezaY23zmcXgrpIh9DbXY2WVgdq7K4d6e2uM57i4CyGzrS0wyTo68OIkTpPA8ah9xHp2NNPn19OJ3PH2aDH6GLmvMUBU_MjTV4ew2PyoDFtxCd384x8uLz4uHqVXb-7er16cZ05qXWelTlqW1eNNgKbklWVQiZzUbI0GeNcqlIVVjWyKbFUzbK2StuqsBK1FCjPyPOj67CrOrR1ej-YFobgOhMO0BsH_yPe3cKm_wFCay5Zngye_mswK__-YSI8OxJ-uhYPM84ZTFnBFqYYYIoEpqzgT1awh_Xq5n3akj476l0ccT_rTfgOeSELBV_eXsEbdcP4pV7DN_kbos2axA</recordid><startdate>201006</startdate><enddate>201006</enddate><creator>Gledhill, Karl</creator><creator>Rhodes, Lesley E.</creator><creator>Brownrigg, Margaret</creator><creator>Haylett, Ann K.</creator><creator>Masoodi, Mojgan</creator><creator>Thody, Anthony J.</creator><creator>Nicolaou, Anna</creator><creator>Tobin, Desmond J.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>201006</creationdate><title>Prostaglandin-E2 is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner</title><author>Gledhill, Karl ; Rhodes, Lesley E. ; Brownrigg, Margaret ; Haylett, Ann K. ; Masoodi, Mojgan ; Thody, Anthony J. ; Nicolaou, Anna ; Tobin, Desmond J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3886-96e8dcbf8a2ef90bb5e0362905e0001135957d5f3f9e95f4cd58db7d3e832e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Arachidonic Acid - pharmacology</topic><topic>cyclooxygenase</topic><topic>Dinoprostone - biosynthesis</topic><topic>Epidermal Cells</topic><topic>epidermal melanocyte</topic><topic>Female</topic><topic>Humans</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Indomethacin - pharmacology</topic><topic>Intramolecular Oxidoreductases - metabolism</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Keratinocytes - radiation effects</topic><topic>Male</topic><topic>Melanins - biosynthesis</topic><topic>Melanocytes - drug effects</topic><topic>Melanocytes - enzymology</topic><topic>Melanocytes - metabolism</topic><topic>Melanocytes - radiation effects</topic><topic>melanogenesis</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Monophenol Monooxygenase - metabolism</topic><topic>Nitrobenzenes</topic><topic>Original</topic><topic>Oxidoreductases - metabolism</topic><topic>prostaglandin E2</topic><topic>skin phototype</topic><topic>Sulfonamides</topic><topic>ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gledhill, Karl</creatorcontrib><creatorcontrib>Rhodes, Lesley E.</creatorcontrib><creatorcontrib>Brownrigg, Margaret</creatorcontrib><creatorcontrib>Haylett, Ann K.</creatorcontrib><creatorcontrib>Masoodi, Mojgan</creatorcontrib><creatorcontrib>Thody, Anthony J.</creatorcontrib><creatorcontrib>Nicolaou, Anna</creatorcontrib><creatorcontrib>Tobin, Desmond J.</creatorcontrib><collection>Istex</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Pigment cell and melanoma research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gledhill, Karl</au><au>Rhodes, Lesley E.</au><au>Brownrigg, Margaret</au><au>Haylett, Ann K.</au><au>Masoodi, Mojgan</au><au>Thody, Anthony J.</au><au>Nicolaou, Anna</au><au>Tobin, Desmond J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prostaglandin-E2 is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner</atitle><jtitle>Pigment cell and melanoma research</jtitle><addtitle>Pigment Cell Melanoma Res</addtitle><date>2010-06</date><risdate>2010</risdate><volume>23</volume><issue>3</issue><spage>394</spage><epage>403</epage><pages>394-403</pages><issn>1755-1471</issn><eissn>1755-148X</eissn><abstract>Summary
Excessive ultraviolet radiation (UVR) exposure induces erythema, mediated in part by prostaglandin‐E2 (PGE2). While keratinocytes are a major PGE2 source, epidermal melanocytes (EM) also express PGE2‐production machinery. It is unclear whether EM‐produced PGE2 contributes to UVR‐induced skin inflammation, and whether this is correlated with melanogenesis. Epidermal melanocytes were cultured from skin phototype‐1 and ‐4 donors, followed by assessment of PGE2 production and melanogenesis. Epidermal melanocytes expressed cytoplasmic phospholipase‐A2, cyclooxygenase‐1, cytoplasmic prostaglandin‐E synthase and microsomal prostaglandin‐E synthase‐1, ‐2. Epidermal melanocytes produced PGE2 under basal conditions, which increased further after arachidonic acid stimulation. Epidermal melanocytes expressed cyclooxygenase‐2 (COX‐2) mRNA and a selective COX‐2 inhibitor (NS‐398) reduced PGE2 production. Ultraviolet B‐induced PGE2 production was positively correlated with skin phototype‐1, despite variability between individual EM donors. By contrast, there was no correlation between PGE2 production by EM and their melanogenic status. Thus, EM may contribute to UVR‐induced erythema, with role of donor skin phototype more important than their melanogenic status.
View the pubcast on this paper at http://www.scivee.tv/node/18147</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20236442</pmid><doi>10.1111/j.1755-148X.2010.00696.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Arachidonic Acid - pharmacology cyclooxygenase Dinoprostone - biosynthesis Epidermal Cells epidermal melanocyte Female Humans Hydrogen Peroxide - pharmacology Indomethacin - pharmacology Intramolecular Oxidoreductases - metabolism Keratinocytes - drug effects Keratinocytes - metabolism Keratinocytes - radiation effects Male Melanins - biosynthesis Melanocytes - drug effects Melanocytes - enzymology Melanocytes - metabolism Melanocytes - radiation effects melanogenesis Membrane Glycoproteins - metabolism Monophenol Monooxygenase - metabolism Nitrobenzenes Original Oxidoreductases - metabolism prostaglandin E2 skin phototype Sulfonamides ultraviolet radiation Ultraviolet Rays Young Adult |
| title | Prostaglandin-E2 is produced by adult human epidermal melanocytes in response to UVB in a melanogenesis-independent manner |
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